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All Journal Journal of Innovation in Community Empowerment (JICE) Journal of Biotechnology and Natural Science
Junita, Fina
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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The Effect of Ethanol Concentration Variations on The Total Phenolic And Flavonoid Levels of Bauhinia purpurea L. Leaf Extract Nurhasanah, Devika; Ulvia, Rengganis; Junita, Fina
Journal of Biotechnology and Natural Science Vol. 4 No. 2 (2024): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v4i2.12060

Abstract

Bauhinia purpurea L. is an ornamental plant that is not widely known or explored by the public, despite its significant antioxidant properties. Phenolic compounds and flavonoids, known for their antioxidant activity, can be extracted from the leaves through maceration with 70% ethanol and 96% ethanol. The difference in solvent polarity between these two ethanol concentrations affects the solubility of bioactive compounds. This study aimed to evaluate the effect of 70% and 96% ethanol concentrations Bauhinia purpurea L on the total phenolic and flavonoid levels in. An experimental method was employed, using Bauhinia purpurea L powder macerated with 70% and 96% ethanol, followed by evaporation at 40°C-50°C to obtain thick extracts. The extracts were subjected to organoleptic tests, phytochemical screening, Thin Layer Chromatography (TLC), and UV-Vis spectrophotometry. Total phenolic content was determined using gallic acid standards (mg GAE/g extract), while total flavonoid content was measured using quercetin standards (mg QE/g extract). The organoleptic test revealed that both the 70% and 96% ethanol extracts of Bauhinia purpurea L had a blackish-green color, thick consistency, distinctive odor, and bland taste. Phytochemical screening indicated the presence of phenolics, flavonoids, and saponins. The total phenolic content for the 70% ethanol extract was 14.644 ± 0.222 mg GAE/g, and the total flavonoid content was 25.519 ± 0.921 mg QE/g. The total phenolic content for the 96% ethanol extract was 7.176 ± 0.347 mg GAE/g, and the total flavonoid content was 11.208 ± 0.412 mg QE/g. These results indicate a significant difference between the use of 70% and 96% ethanol in extracting total phenolic and flavonoid content from Bauhinia purpurea L, with the 70% ethanol extract showing higher levels of both compounds.
Upaya Peningkatan Pengetahuan Tentang Penyakit Menular Seksual (PMS) Terutama yang Disebabkan Oleh Seks Bebas Remaja di Dukuh Manggung, Sumberagung Junita, Fina; Ardianto, Lisman Septa; Putri, Anggraeni Widya; Azzahra, Cindy; Husna, Lailatul; Sabilah, Nakkyah Sal; Azizah, Nuriyatul; Wulandari, Sephia; Novitasari, Trilia; Khusna, Zella Nufusul; Purnomosari, Kurnia Rahayu; Ulvia, Rengganis
The Journal of Innovation in Community Empowerment Vol 7 No 1 (2025): Journal of Innovation in Community Empowerment (JICE)
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jice.v7i1.1436

Abstract

Seksualitas merupakan aspek yang berkaitan dengan reproduksi serta mencakup dimensi fisik, biologis, psikologis, dan sosial. Remaja perlu memiliki kesadaran positif terhadap seksualitas agar dapat membentuk perilaku yang sehat dan menjadi individu yang matang secara jasmani maupun rohani. Kesalahpahaman tentang seksualitas umumnya disebabkan oleh kurangnya pengetahuan, misalnya anggapan bahwa alat kelamin merupakan bagian tubuh yang kotor dan tidak boleh disentuh. Globalisasi dan kebebasan informasi telah memicu perubahan perilaku seksual yang tidak sehat, yang berdampak pada berbagai masalah kesehatan reproduksi, seperti pernikahan dini, kehamilan di usia remaja, kehamilan tidak diinginkan, aborsi, penyakit menular seksual, HIV/AIDS, infertilitas, dan kanker organ reproduksi. Kegiatan ini bertujuan untuk meningkatkan pengetahuan remaja Dukuh Manggung mengenai Penyakit Menular Seksual (PMS). Materi yang disampaikan meliputi pengenalan PMS, khususnya yang berkaitan dengan perilaku seks bebas, agar remaja memahami cara menjaga kesehatan reproduksi, mengenali gejala, serta melakukan tindakan pencegahan. Pemahaman peserta diukur melalui hasil pretest dan posttest yang masing-masing terdiri dari 10 soal pilihan ganda. Hasil pretest menunjukkan bahwa 16 peserta (55,17%) memiliki pengetahuan yang baik mengenai PMS, terutama yang disebabkan oleh seks bebas. Setelah pemberian materi oleh pemateri, terjadi peningkatan pengetahuan, yang terlihat dari hasil posttest, di mana sebanyak 18 peserta (62,1%) menunjukkan pemahaman yang baik terhadap PMS.
Co-Authors Ardianto, Lisman Septa Azizah, Nuriyatul Azzahra, Cindy Devika Nurhasanah Khusna, Zella Nufusul Lailatul Husna Novitasari, Trilia Putri, Anggraeni Widya Sabilah, Nakkyah Sal Sari, Kurnia Rahayu Purnomo Ulvia, Rengganis Wulandari, Sephia