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All Journal Indonesian Journal of Biotechnology SAINS TANAH - Journal of Soil Science and Agroclimatology Jurnal Penelitian Kelapa Sawit
Nugroho, Syarul
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Materials Science & Nanotechnology

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A robust in planta Agrobacterium‐mediated transformation in red chili (Capsicum annuum L.) Hamdani, Anti Damayanti; Nugroho, Syarul; Esyanti, Rizkita Rachmi; Faizal, Ahmad; Suhandono, Sony
Indonesian Journal of Biotechnology Vol 29, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.94653

Abstract

Plant improvement through in vitro culture and genetic engineering is a significant aspect of breeding programs aimed at producing disease‐resistant cultivars of disease‐prone red chili (Capsicum annuum L.). However, the Capsicum genus is recalcitrant to genetic transformation and in vitro regeneration. Moreover, developing a universal transformation protocol is difficult due to its highly genotype‐dependent nature. Therefore, this study aimed to develop an Agrobacterium‐mediated in planta transformation method applicable to various red chili cultivars. Two open‐pollinated varieties, Tanjung 2 and Ciko, were subjected to transformation. The young seedlings were immersed in transformation medium containing Agrobacterium tumefaciens strain GV3101 harboring the binary vector pCAMBIA1301, which carries the β‐glucuronidase (GUS) gene. GUS histochemical analysis revealed that all the primary transformants of Tanjung 2 and Ciko were identified as chimeric. The average staining in the body of the seedlings was 88.63 + 26.33% in Tanjung 2, and 90.65 + 16.77% in the Ciko variety. More than 50% of the seedlings continued to express GUS in their shoot areas 10 days after Agrobacterium infection, indicating the possibility of transgene inheritance in the following generation. The in planta transformation approach is notably genotype independent, making it a promising standard transformation protocol for different red chili varieties.
Analisis Single Nucleotide Polymorphism (SNP) Gen Laccase-24 (EgLCC24) dalam Ketahanan Kelapa Sawit (Elaeis guineensis Jacq.) terhadap Ganoderma boninense Nugroho, Syarul; Yuli Rahmadi, Hernawan; Syukur, Muhamad; Widodo, Widodo; Bayuardi Suwarno, Willy; Sri Wening, Sri Wening; Faizah, Rokhana; Diah Setiowati, Retno; Adriwan Siregar, Heri
Jurnal Penelitian Kelapa Sawit Vol 33 No 1 (2025): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v33i1.285

Abstract

Ganoderma boninense attack and its rapid spread in oil palm plantations have caused significant economic losses. The use of Ganoderma resistant plant materials can be a solution, but the allelic segregation that occurs causes not all DxP progeny obtained have the nature of resistance to Ganoderma. The role of molecular markers is needed to assist initial selection of Ganoderma resistant DxP progenies in nurseries. This study aims to identify targeted SNP markers from the Laccase-24 gene (EgLCC24), which is thought to play a role in oil palm resistance to Ganoderma. Three oil palm DxP populations were used in this study, namely populations A, B, and C. Ganoderma screening in nurseries was carried out on these three populations to obtain progeny samples with resistant and susceptible phenotypes, which were then further analyzed. The methods used include SNP analysis and the use of SNAP primers for marker development. The SNP differences obtained from this study led to amino acid changes, but did not cause stop codons. The validation results using SNAP primers on the Laccase-24 gene showed that DxP progeny from populations A, B, and C relatively have moderate allele characteristics of Ganoderma resistance.
Effects of mounding on soil properties, root development, and physiological responses of Ganoderma boninense-infected and uninfected oil palms Syarovy, Muhdan; Sumaryanto, Sumaryanto; Nugroho, Syarul; Siregar, Valdi Muhamad Rafiansyah; Wijayanti, Eka
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 22, No 2 (2025): December
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v22i2.94443

Abstract

Basal stem rot (BSR), caused by Ganoderma boninense, is a major disease in oil palm plantations, leading to significant losses. Currently, there is no fully effective method to control this disease. This study evaluated the effects of mounding techniques on root development in both Ganoderma-infected and uninfected oil palms over 8 months. The research was conducted across six plantations in North Sumatra, where root biomass was measured by uncovering a quarter of the mounded area and analyzing the soil's physical and chemical properties. The results showed that, although most soil properties were comparable, cation exchange capacity (CEC) and magnesium (Mg) levels were higher in uninfected palms. Root growth increased in both infected and uninfected palms from three to eight months after mounding, but by the seventh and eighth months, the root biomass in infected palms was lower compared to uninfected palms. Infected palms primarily showed the growth of primary-like roots, whereas uninfected palms exhibited more extensive tertiary and quaternary roots. The study concluded that while mounding stimulates root development, Ganoderma infection limits this growth. These findings have important implications for improving disease management strategies in oil palm plantations.
Co-Authors Adriwan Siregar, Heri Ahmad Faizal Diah Setiowati, Retno Eka Wijayanti Hamdani, Anti Damayanti Muhamad Syukur Muhdan Syarovy RIZKITA RACHMI ESYANTI Rokhana Faizah Siregar, Valdi Muhamad Rafiansyah SONY SUHANDONO Sri Wening Sri Wening, Sri Wening Sumaryanto Sumaryanto, Sumaryanto Widodo Widodo Willy Bayuardi Suwarno Yuli Rahmadi, Hernawan