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All Journal Indonesian Journal of Biotechnology Jurnal Penelitian Kelapa Sawit
Nugroho, Syarul
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

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A robust in planta Agrobacterium‐mediated transformation in red chili (Capsicum annuum L.) Hamdani, Anti Damayanti; Nugroho, Syarul; Esyanti, Rizkita Rachmi; Faizal, Ahmad; Suhandono, Sony
Indonesian Journal of Biotechnology Vol 29, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.94653

Abstract

Plant improvement through in vitro culture and genetic engineering is a significant aspect of breeding programs aimed at producing disease‐resistant cultivars of disease‐prone red chili (Capsicum annuum L.). However, the Capsicum genus is recalcitrant to genetic transformation and in vitro regeneration. Moreover, developing a universal transformation protocol is difficult due to its highly genotype‐dependent nature. Therefore, this study aimed to develop an Agrobacterium‐mediated in planta transformation method applicable to various red chili cultivars. Two open‐pollinated varieties, Tanjung 2 and Ciko, were subjected to transformation. The young seedlings were immersed in transformation medium containing Agrobacterium tumefaciens strain GV3101 harboring the binary vector pCAMBIA1301, which carries the β‐glucuronidase (GUS) gene. GUS histochemical analysis revealed that all the primary transformants of Tanjung 2 and Ciko were identified as chimeric. The average staining in the body of the seedlings was 88.63 + 26.33% in Tanjung 2, and 90.65 + 16.77% in the Ciko variety. More than 50% of the seedlings continued to express GUS in their shoot areas 10 days after Agrobacterium infection, indicating the possibility of transgene inheritance in the following generation. The in planta transformation approach is notably genotype independent, making it a promising standard transformation protocol for different red chili varieties.
Analisis Single Nucleotide Polymorphism (SNP) Gen Laccase-24 (EgLCC24) dalam Ketahanan Kelapa Sawit (Elaeis guineensis Jacq.) terhadap Ganoderma boninense Nugroho, Syarul; Yuli Rahmadi, Hernawan; Syukur, Muhamad; Widodo, Widodo; Bayuardi Suwarno, Willy; Sri Wening, Sri Wening; Faizah, Rokhana; Diah Setiowati, Retno; Adriwan Siregar, Heri
Jurnal Penelitian Kelapa Sawit Vol 33 No 1 (2025): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v33i1.285

Abstract

Ganoderma boninense attack and its rapid spread in oil palm plantations have caused significant economic losses. The use of Ganoderma resistant plant materials can be a solution, but the allelic segregation that occurs causes not all DxP progeny obtained have the nature of resistance to Ganoderma. The role of molecular markers is needed to assist initial selection of Ganoderma resistant DxP progenies in nurseries. This study aims to identify targeted SNP markers from the Laccase-24 gene (EgLCC24), which is thought to play a role in oil palm resistance to Ganoderma. Three oil palm DxP populations were used in this study, namely populations A, B, and C. Ganoderma screening in nurseries was carried out on these three populations to obtain progeny samples with resistant and susceptible phenotypes, which were then further analyzed. The methods used include SNP analysis and the use of SNAP primers for marker development. The SNP differences obtained from this study led to amino acid changes, but did not cause stop codons. The validation results using SNAP primers on the Laccase-24 gene showed that DxP progeny from populations A, B, and C relatively have moderate allele characteristics of Ganoderma resistance.
Co-Authors Adriwan Siregar, Heri Ahmad Faizal Diah Setiowati, Retno Hamdani, Anti Damayanti Muhamad Syukur RIZKITA RACHMI ESYANTI Rokhana Faizah SONY SUHANDONO Sri Wening Sri Wening, Sri Wening Widodo Widodo Willy Bayuardi Suwarno Yuli Rahmadi, Hernawan