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All Journal Seminar Nasional Lahan Suboptimal Jurnal Ilmiah Ilmu Terapan Universitas Jambi
Inaya, Rihani
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Agriculture, Biological Sciences & Forestry Education Engineering Industrial & Manufacturing Engineering Other

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Efektivitas Beberapa Metode Sterilisasi terhadap Tingkat Kontaminasi Eksplan Tulang Daun Duku pada Kultur In-Vitro Inaya, Rihani; Irmawati, Irmawati; Susilawati, Susilawati; Helen, Helen; Herlin, Weri; Negara, Zaidan P.
Seminar Nasional Lahan Suboptimal Vol 12, No 1 (2024): Vol 12, No 1 (2024): Prosiding Seminar Nasional Lahan Suboptimal ke-12 “Revital
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Inaya, R., Irmawati, I., Susilawati, S., Helen, H., Herlin, W., & Negara, Z. P. (2024). The effect of different sterilization techniques on the contamination levels of duku leaf midrib explant in In-Vitro Culture. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-12 Tahun 2024, Palembang 21 Oktober 2024. (pp. 667–678). Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).The Duku plant (Lansium domesticum) is one of the popular tropical fruit in indonesia. Using the idea of plant cells' totipotency, tissue culture is an alternate method for a quick and massive propagation of high-quality duku plant seedlings. This research was aimed to evaluate different sterilization materials and procedures on duku leaf midrib explants through tissue culture. The research was conducted from June to September 2024 at Tissue Culture Laboratory, Department  of  Agronomy, Universitas Sriwijaya (2°59’23.4”S 104°43’53.4”E). A combination of  sterilizing substances, including 70% alcohol, fungicide, bactericide, liquid detergent, and NaOCl, were utilized in this sterilization procedures. The percentage of live explants, browning explants, and explants infected with bacteria or fungi then were calculated using parametric descriptive analysis. The results showed that the P3 treatment's sterilant combination liquid detergent for 3 minutes, 0.2% of fungicide and bactericide for 10 minutes, 1% NaOCl for 15 minutes, and 70% alcohol for 5 minute produced the highest percentage of live explants (46%) with a relatively low percentage of contaminated explants (30%) and the lowest percentage of browning explants (23%). Thus P3 treatment was concluded as the best treatment to reduce the contamination level and browning of duku leaf midrib explant.
OPTIMIZING SURFACE STERILIZATION OF Lansium domesticum LEAF LAMINA EXPLANTS USING SODIUM HYPOCHLORITE Irmawati, Irmawati; Susilawati, Susilawati; Helen, Helen; Inaya, Rihani; Herlin, Weri
Jurnal Ilmiah Ilmu Terapan Universitas Jambi Vol. 10 No. 2 (2026): Volume 10, Nomor 2, April 2026
Publisher : LPPM Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiituj.v10i2.54331

Abstract

Lansium domesticum Corr. is a fruit crop with high consumer demand; however, its conventional propagation is relatively slow. In vitro culture offers a potential alternative to accelerate plant propagation. This study aimed to evaluate sodium hypochlorite (NaOCl)-based sterilization protocols and identify the most effective treatment for in vitro establishment of leaf lamina explants. The experiment was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Sriwijaya, from July to September 2024 using five sterilization treatments with different NaOCl concentrations and exposure times. Data were analyzed descriptively to determine the percentages of bacterial contamination, fungal contamination, surviving explants, and browning incidence. The results showed that the treatment consisting of liquid detergent (3 min), 0.2% streptomycin sulfate (10 min), 0.2% benomyl (10 min), 1% NaOCl (15 min), and 70% ethanol (5 min) was the most effective protocol. This treatment resulted in 63% explant survival, 0% browning, 33% bacterial contamination, and 3% fungal contamination. This study provides a practical sterilization protocol that balances antimicrobial effectiveness and tissue viability, representing a novel contribution for in vitro propagation of Lansium domesticum. The findings have important implications for improving large-scale propagation efficiency of this economically important tropical fruit species. These results indicate that moderate NaOCl exposure is essential to achieve an optimal balance between contamination control and explant viability in in vitro culture systems.
Co-Authors Helen, Helen Irmawati Irmawati Irmawati Irmawati Negara, Zaidan P. Susilawati, Susilawati Weri Herlin