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All Journal Jurnal Hortikultura Indonesia (JHI) Seminar Nasional Lahan Suboptimal Jurnal Integritas Serasan Sekundang
Negara, Zaidan P.
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Social Sciences Other

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Efektivitas Beberapa Metode Sterilisasi terhadap Tingkat Kontaminasi Eksplan Tulang Daun Duku pada Kultur In-Vitro Inaya, Rihani; Irmawati, Irmawati; Susilawati, Susilawati; Helen, Helen; Herlin, Weri; Negara, Zaidan P.
Seminar Nasional Lahan Suboptimal Vol 12, No 1 (2024): Vol 12, No 1 (2024): Prosiding Seminar Nasional Lahan Suboptimal ke-12 “Revital
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Inaya, R., Irmawati, I., Susilawati, S., Helen, H., Herlin, W., & Negara, Z. P. (2024). The effect of different sterilization techniques on the contamination levels of duku leaf midrib explant in In-Vitro Culture. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-12 Tahun 2024, Palembang 21 Oktober 2024. (pp. 667–678). Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).The Duku plant (Lansium domesticum) is one of the popular tropical fruit in indonesia. Using the idea of plant cells' totipotency, tissue culture is an alternate method for a quick and massive propagation of high-quality duku plant seedlings. This research was aimed to evaluate different sterilization materials and procedures on duku leaf midrib explants through tissue culture. The research was conducted from June to September 2024 at Tissue Culture Laboratory, Department  of  Agronomy, Universitas Sriwijaya (2°59’23.4”S 104°43’53.4”E). A combination of  sterilizing substances, including 70% alcohol, fungicide, bactericide, liquid detergent, and NaOCl, were utilized in this sterilization procedures. The percentage of live explants, browning explants, and explants infected with bacteria or fungi then were calculated using parametric descriptive analysis. The results showed that the P3 treatment's sterilant combination liquid detergent for 3 minutes, 0.2% of fungicide and bactericide for 10 minutes, 1% NaOCl for 15 minutes, and 70% alcohol for 5 minute produced the highest percentage of live explants (46%) with a relatively low percentage of contaminated explants (30%) and the lowest percentage of browning explants (23%). Thus P3 treatment was concluded as the best treatment to reduce the contamination level and browning of duku leaf midrib explant.
The The Effectiveness of Different Sterilant Combination on Citrullus lanatus L. In Vitro: Uji Efektivitas Kombinasi Bahan Sterilan yang Berbeda pada Citrullus lanatus L. secara In Vitro Indah Lestari, Laras; Irmawati; Negara, Zaidan P.; Hasmeda, Mery; Sulaiman, Firdaus; Sulistyaningsih, Lidwina N.; Marlina
Jurnal Hortikultura Indonesia (JHI) Vol. 16 No. 2 (2025): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.16.2.125-135

Abstract

 Watermelon plants require consistent environmental conditions throughout their growth period. Tissue culture serves as a solution in plant propagation techniques that can control the environmental conditions for watermelon growth. This study aims to determine the appropriate sterilization method and explant type for watermelon plant propagation using tissue culture techniques. The research was conducted from June to August 2022 at the Tissue Culture Laboratory of the Faculty of Agriculture, Sriwijaya University. This study used a Complete Randomized Factorial Design (CRFD) with two factors: six treatments of different sterilization agent sequences for explants, including Benomyl 0.2% for 3 minutes, detergent for 3 minutes, HgCl₂ 0.03% for 10 minutes, and 70% alcohol for 45 seconds, and four types of explants: radicle, hypocotyl, plumule, and cotyledon, with two replicates of five experimental units each. The results showed that sterilization factors and explant types had a significant effect on the percentage of contaminated explants and indicated a decrease in contamination in the treatment of 0.2% benomyl for 3 minutes, detergent for 3 minutes, 0.03% HgCl2 for 10 minutes, and 70% alcohol for 45 seconds on cotyledon explants. Keywords: browning, contamination, tissue culture, watermelon sprout
PENGARUH KERAPATAN POPULASI PAKCOY (Brassica rapa L.) PADA HIDROPONIK SISTEM RAKIT APUNG Sodikin, Erizal; Harun, M. Umar; Negara, Zaidan P.; Afrizal, Jeri
Jurnal Integritas Serasan Sekundang Vol 4 No 1 (2022): Jurnal Integritas Serasan Sekundang
Publisher : Badan Penelitian dan Pengembangan Daerah KabupatenMuara Enim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64987/jiss.v4i1.48

Abstract

The research was on Saung Hydoponic Bedeng Putih Indralaya from April until June, 2022. The purpose of the study was to determine the best spacing for pakcoy cultivation in hydroponic floating systems. The research activited carried out included installing mulch on styrofoam, seeding seeds, transplanting, observing plant, providing AB mix nutrition, checking ppm, pH, and water temperature, controlling pests and diseases plants, cleaning weeds in hydroponic areas, and harvesting. Pakcoy cultivation on styrofoam measuring 1.5 m x 0.5 m in pond the area 9 m x 1.5 m. Based on observations, cultivation of pakcoy in floating systems the spacing of 25 cm x 20 cm or 10 plants in styrofoam resulted in the plant height of 26.60 cm, number of leaves were 18 , canopy area was 28.75 cm², fresh weight of plant was 210.40 g, and the percentage of plants worth selling was 100%.
Co-Authors Afrizal, Jeri Erizal Sodikin Firdaus Sulaiman, Firdaus Helen, Helen Inaya, Rihani Indah Lestari, Laras Irmawati Irmawati Irmawati M. Umar Harun Marlina Mery Hasmeda Sulistyaningsih, Lidwina N. Susilawati, Susilawati Weri Herlin