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All Journal Jurnal Kesehatan Journal of Noncommunicable Diseases (JOND)
Primastuti, Yuridistya Putri
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Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Optimization of Complete Blood Count Results with Variations in Specimen Handling and Whole Blood Secondary Homogenization Techniques Aliviameita, Andika; Puspitasari, Puspitasari; Primastuti, Yuridistya Putri; Batis, Afifah
Jurnal Kesehatan Vol 15 No 2 (2024): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v15i2.4578

Abstract

Specimen homogenization and the addition of anticoagulants are pre-analytical steps that influence the accuracy of the test results. Complete blood count (CBC) is a screening test that supports disease diagnosis and aids in determining the appropriate therapy for patients. Inadequate specimens are inappropriate for subsequent testing, as they can lead to inaccurate results in the CBC examination. This study aims to determine the optimization of complete blood count results with variations in specimen handling and secondary homogenization techniques for whole blood. The type of research used is quantitative analysis using laboratory experimental methods. The study was conducted in May 2024 in the Clinical Pathology Laboratory of the Diploma-4 Medical Laboratory Technology Study Program, Faculty of Health Sciences, Muhammadiyah University of Sidoarjo, using 48 blood samples. The results of the MANOVA test indicate that the type of anticoagulant has a significant effect on the erythrocyte count (p=0.041) but does not have a significant impact on the leukocyte count (p=0.844) and platelet count (p=0.920). Meanwhile, the homogenization technique does not significantly affect the erythrocyte count (p=0.959), leukocyte count (p=0.991), or platelet count (p=0.867). This study concludes that the secondary homogenization technique has no significant effect. In contrast, the type of anticoagulant significantly impacts the results of the Complete Blood Count (CBC) test. This research suggests collecting whole blood specimens using Ethylene Diamine Tetra Acetate (EDTA) vacutainers as the anticoagulant and applying a secondary homogenization technique before performing the CBC analysis.
Perbedaan Variasi Teknik Homogenisasi dengan Antikoagulan EDTA Vacutainer dan Konvensional Terhadap Jumlah Leukosit dan Trombosit Primastuti, Yuridistya Putri; Aliviameita, Andika
JOURNAL OF NONCOMMUNICABLE DISEASES Vol 5, No 1 (2025): April 2025
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jond.v5i1.1128

Abstract

Pemeriksaan hematologi membutuhkan penambahan antikoagulan pada sampel darah dengan teknik homogenisasi tertentu untuk mencegah adanya bekuan darah. Tujuan penelitian ini untuk mengetahui perbedaan variasi teknik homogenisasi pada sampel darah dengan antikoagulan EDTA vacutainer dan konvensional terhadap jumlah leukosit dan trombosit. Penelitian dilaksanakan pada bulan Mei 2024 di laboratorium patologi klinik Prodi D-IV Teknologi Laboratorium Medis Universitas Muhammadiyah Sidoarjo secara kuantitatif dengan jenis penelitian eksperimental laboratorik. Sampel yang digunakan berjumlah 32 dari 8 responden. Pemeriksaan jumlah leukosit dan trombosit menggunakan metode otomatis dengan alat Hematology Analyzer. Data hasil penelitian diuji secara statistik. Hasil pemeriksaan jumlah leukosit menggunakan uji Dependent T Test, sedangkan hasil pemeriksaan jumlah trombosit menggunakan uji Wilcoxon. Kesimpulan penelitian ini adalah tidak terdapat perbedaan hasil pemeriksaan jumlah leukosit dan trombosit yang dilakukan dengan homogenisasi sekunder inversi 4 kali dan 8 kali pada antikoagulan EDTA vacutainer dan konvensional (p > 0,05).Hematological examination requires the addition of anticoagulants to blood samples using specific homogenization techniques to prevent clot formation. The aim of this study was to determine the differences in variations of homogenization techniques on blood samples with EDTA vacutainers and conventional anticoagulants in relation to leukocyte and platelet counts. The research was conducted in May 2024 at the Clinical Pathology Laboratory of the Medical Laboratory Technology D-IV Program, Universitas Muhammadiyah Sidoarjo, using a quantitative approach with a laboratory experimental research design. We used a total of 32 samples from 8 respondents. Leukocyte and platelet counts were examined using an automated method with a hematology analyzer. The research data were statistically tested. The leukocyte count results were analyzed using the dependent t-test, while the platelet count results were analyzed using the Wilcoxon test. The conclusion of the study is that there were no differences in the results of leukocyte and platelet counts using secondary homogenization by inversion 4 times and 8 times with EDTA vacutainers and conventional anticoagulants (p > 0.05).
Co-Authors Andika Aliviameita Batis, Afifah Puspitasari Puspitasari