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Batis, Afifah
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Optimization of Complete Blood Count Results with Variations in Specimen Handling and Whole Blood Secondary Homogenization Techniques Aliviameita, Andika; Puspitasari, Puspitasari; Primastuti, Yuridistya Putri; Batis, Afifah
Jurnal Kesehatan Vol 15 No 2 (2024): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v15i2.4578

Abstract

Specimen homogenization and the addition of anticoagulants are pre-analytical steps that influence the accuracy of the test results. Complete blood count (CBC) is a screening test that supports disease diagnosis and aids in determining the appropriate therapy for patients. Inadequate specimens are inappropriate for subsequent testing, as they can lead to inaccurate results in the CBC examination. This study aims to determine the optimization of complete blood count results with variations in specimen handling and secondary homogenization techniques for whole blood. The type of research used is quantitative analysis using laboratory experimental methods. The study was conducted in May 2024 in the Clinical Pathology Laboratory of the Diploma-4 Medical Laboratory Technology Study Program, Faculty of Health Sciences, Muhammadiyah University of Sidoarjo, using 48 blood samples. The results of the MANOVA test indicate that the type of anticoagulant has a significant effect on the erythrocyte count (p=0.041) but does not have a significant impact on the leukocyte count (p=0.844) and platelet count (p=0.920). Meanwhile, the homogenization technique does not significantly affect the erythrocyte count (p=0.959), leukocyte count (p=0.991), or platelet count (p=0.867). This study concludes that the secondary homogenization technique has no significant effect. In contrast, the type of anticoagulant significantly impacts the results of the Complete Blood Count (CBC) test. This research suggests collecting whole blood specimens using Ethylene Diamine Tetra Acetate (EDTA) vacutainers as the anticoagulant and applying a secondary homogenization technique before performing the CBC analysis.
Variations in Erythrocyte Count and Hemoglobin Levels in Blood Samples Collected Using Conventional Anticoagulants and Vacutainers with Different Secondary Homogenization Techniques Batis, Afifah; Aliviameita, Andika
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 9 No 2 (2025): Medical Technology and Public Health Journal September 2025
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v9i2.7006

Abstract

The choice of anticoagulants and the methods of homogenization are critical pre-analytical steps that can significantly influence the accuracy of laboratory results. This study aims to assess the differences in erythrocyte count and hemoglobin levels among blood samples processed with conventional anticoagulants and Vacutainer tubes, taking into account variations in secondary homogenization techniques. A laboratory experimental design was employed, utilizing 32 blood samples acquired from eight participants. The analysis was conducted using an automated hematology analyzer. Data were subjected to a Paired T-Test, indicating no significant differences in erythrocyte counts (p=0.617) and hemoglobin levels (p=0.510) between conventional EDTA samples that were homogenized four times and those homogenized eight times. However, a significant difference was noted in erythrocyte counts (p=0.018), while hemoglobin levels remained unaffected (p=0.393) in EDTA vacutainer samples subjected to four versus eight rounds of secondary homogenization. Additionally, a significant difference was found in both erythrocyte counts (p = 0.008) and hemoglobin levels (p < 0.0001) between conventional EDTA samples and those subjected to four rounds of secondary homogenization using vacutainers. Similarly, there was a significant difference in erythrocyte counts (p = 0.006) and hemoglobin levels (p = 0.014) between conventional EDTA samples and Vacutainer samples that underwent eight rounds of homogenization.  Keywords: Blood Specimen Collection; EDTA; Erythrocyte; Hemoglobin; Secondary Homogenization
Co-Authors Andika Aliviameita Primastuti, Yuridistya Putri Puspitasari Puspitasari