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All Journal Universitas Muhammadiyah Yogyakarta Undergraduate Conference Proceeding Prosiding Seminar Nasional Program Pengabdian Masyarakat
Rivaldi, Fajar
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Optimization of pH to Bacteriocin Production by Lactobacillus Acidophillus Against Methicillin-Resistant Staphylococcus Aureus Rivaldi, Fajar; Suryani, Lilis
Proceedings of Universitas Muhammadiyah Yogyakarta Graduate Conference Vol. 4 No. 2 (2025): Renewable Energy, Environment, and Technology for Sustainable Transformation
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/umygrace.v4i2.667

Abstract

Introduction: Methicillin-Resistant Staphylococcus aureus (MRSA) is a strain of Staphylococcus aureus that has developed resistance to the antibiotic’s methicillin and beta lactams. MRSA has infected approximately 11 million people, and causes skin and soft tissue infections, bacteremia, pneumonia, osteomyelitis, endocarditis, meningitis, and sepsis. MRSA can be transmitted through hands, contact between patients and other people, nurses or doctors who do not wash their hands, and even health workers who have MRSA colonies on their hands and front of the nose without symptoms. Purpose: This study aims to determine the effect of pH on the activity of bacteriocins produced by Lactobacillus Acidophilus against MRSA. Methods: This research uses quasi-experimental laboratory methods. Lactobacillus acidophilus was grown in liquid MRS media for 48 hours. Bacteriocin was obtained by centrifuging liquid MRS containing bacteria at a speed of 6000 rpm for 40 minutes. The liquid formed was tested for its antibacterial effect against MRSA bacteria using the Kirby Bauer method. Bacteriocins were treated with various different pHs, that is; 2, 4, 6, 8, 10. The diameter of the inhibition zone formed during the testing process is measured in millimeters. Data were analyzed using the ANOVA method. Finding: The bacteriocin activity produced by Lactobacillus acidophilus against MRSA showed an average diameter of the inhibition zone of 2.90 mm. The broadest spectrum of antibacterial activity was demonstrated in bacteriocins treated with pH 2. Conclusion: This study shows that pH does not affect the antibacterial activity of bacteriocins produced by Lactobacillus acidophilus against MRSA. Originality/Implication: The results of this study prove that various pH treatments do not affect the ability of bacteriocins produced by L. Acidophilus to inhibit/kill MRSA.
Penyuluhan Kesehatan dalam Menguatkan Kesehatan Masyarakat Dusun Rebobong Lor Syaifurrahman, Arif; Zhafirah, Nabila Novta; Andina, Nova; Rivaldi, Fajar; Septiyanto, Aldi; Diana, Koni; Srinarari, Ardiningrum; Zulfikar, Fikri; Afiq, Faiz Fahmi; Anggreini, Eka Restu; Hilman, Oryzati
Prosiding Seminar Nasional Program Pengabdian Masyarakat Vol. 8 No. 1 (2025): Rekonstruksi Pendidikan di Indonesia
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/ppm.81.1286

Abstract

Rebobong Lor Hamlet has a relatively high prevalence of Non-Communicable Diseases (NCDs), such as hypertension, diabetes, and gout. Environmental factors, including a high smoking rate and a sedentary lifestyle, are the primary contributors to these health problems. Economic limitations often become barriers to raising awareness about the importance of health. Rebobong Lor faces serious challenges in health education, as there is currently no well-organized system in place. The lack of public awareness regarding proper health practices further worsens the situation. Therefore, health education has become an urgent need to support overall community well-being. This community service program serves as a practical implementation of the theoretical knowledge gained by students during their academic studies. In this program, health was chosen as the central theme by the entire community service team. The health education initiative aimed to improve public awareness and knowledge of various health-related issues. The methods used in this outreach included the delivery of educational materials supported by various learning media, such as laptops, sound systems, digital presentations (PowerPoint), and posters. Evaluation results showed an increase in health knowledge among the residents of Rebobong Lor Hamlet following the education sessions on NCDs, particularly hypertension and diabetes mellitus.
Co-Authors Afiq, Faiz Fahmi Andina, Nova Anggreini, Eka Restu Diana, Koni Hilman, Oryzati Lilis Suryani Septiyanto, Aldi Srinarari, Ardiningrum Syaifurrahman, Arif Zhafirah, Nabila Novta Zulfikar, Fikri