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All Journal Biology, Medicine, & Natural Product Chemistry Biopedagogia
Amrullah, Kammal Nizam
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Medicine & Pharmacology Public Health Other

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Antioxidant Activity Test On Ethanol Extract Of Cassava Leaves (Manihot Esculenta C.) Using DPPH Method Husain, Muhammad Hafizh; Febrianti, Dewi Retno; Salsabillah, Salma; Rolita, Ninda Norma; Amrullah, Kammal Nizam; Bawani, Elen Ayu; Ramadhani, Lailatul; Pratiwi, Lidia Wahyu; Yaqin, Ngainul; Jayanti, Angelina; Rafsanjani, Ramizard; Januarista, Terra; Faisal, Faisal; Ramadhan, Majida
Biopedagogia Vol 6, No 2 (2024): November
Publisher : Program Studi Pendidikan Biologi Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/biopedagogia.v6i2.5533

Abstract

Cassava leaves (Manihot esculenta Crantz) are herbs used in traditional medicine. Cassava leaves contain secondary metabolite chemicals such as vitamin C, flavonoids, tannins, saponins, and triterpenes. Cassava leaves are pharmacologically active as anti-inflammatory, antibacterial, and antioxidants. To identify the sample's active antioxidant components using the DPPH technique. The DPPH method is a method of determining the antioxidant activity of a sample by examining its capacity to neutralize free radicals of the 2,2-diphenyl-1-picrylhydrazyl molecule. The antioxidant activity of quercetin from cassava leaf extract (Manihot esculenta Crantz) yielded an IC50 value of 210.33. The IC50 value shows that cassava leaves (Manihot esculenta Crantz) have modest antioxidant lower. Cassava leaves (Manihot esculenta Crantz) have antioxidant activity of 210.33 g/mL so they are included in the weak category in this study.
Antioxidant Activity Analysis Using DPPH and Total Phenol Methods of Soursop Leaf Extract (Annona muricata L.) Wati, Dwi Kartika; Amrullah, Kammal Nizam; Mukarommah, Salsabilatul; Sjakoer, Nour Athiroh Abdoes; Faisal, Faisal; Ramadhan, Majida; Nafisa, Nafisa
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.325-330

Abstract

Soursop leaves (Annona muricata L.) are known to contain various bioactive compounds with potential antioxidant properties. This study aimed to evaluate the antioxidant activity of soursop leaf extract using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method and to determine its total phenolic content using the Folin–Ciocalteu method. Antioxidant activity was assessed at extract concentrations of 500 and 1000 ppm, with quercetin used as a positive control. The results showed that the soursop leaf extract exhibited very strong antioxidant activity, with an IC50 value of 9,486 ppm. The total phenolic content of the extract was relatively high, ranging from 102,929 to 195,702 mg GAE/g extract. The strong antioxidant activity observed is likely associated with the high content of phenolic compounds, which play a crucial role in scavenging free radicals. These findings suggest that soursop leaf extract has significant potential as a natural antioxidant source for applications in the functional food and pharmaceutical industries.
Co-Authors Bawani, Elen Ayu Faisal Faisal Febrianti, Dewi Retno Husain, Muhammad Hafizh Januarista, Terra Jayanti, Angelina Mukarommah, Salsabilatul Nafisa, Nafisa Nour Athiroh Abdoes Sjakoer Pratiwi, Lidia Wahyu Rafsanjani, Ramizard Ramadhan, Majida Ramadhani, Lailatul Rolita, Ninda Norma Salsabillah, Salma Wati, Dwi Kartika Yaqin, Ngainul