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All Journal Biotropika Biology, Medicine, & Natural Product Chemistry JSMARTech : Journal of Smart Bioprospecting and Technology Borneo Journal of Biology Education (BJBE) Biopedagogia
Januarista, Terra
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Other

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Investigation of Total Phenol Content in Red Pomegranate Leaves Extract (Punica granatum L.) Januarista, Terra; Mubarakati, Nurul Jadid; Ramadhan, Majida
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.09

Abstract

Red pomegranate leaves (Punica granatum L.) is one of the plants that has many benefits and potential as herbal medicine. Red pomegranate leaves have bioactivities such as alkaloids, flavonoids, phenols, saponins, tannins and terpenoids so that they can be used as an alternative antioxidant. This study aims to determine the comparison of the amount of total phenol content in red pomegranate leaves extract using maceration and digestion extraction methods. Total phenol content was determined using the Folin Ciocalteau method with a spectrophotometer UV-Vis. The research method carried out was descriptive experimental quantitative and data analysis was carried out using one way ANOVA. The results of the determination of total phenol content in gallic acid obtained a regression equation y = 0.012x + 0.545 with R2 = 0.9981. The results showed that the total phenol content in the ethanol extract of red pomegranate leaves was greater with the maceration method of 91.83 (µg GAE/g sample) compared to the digestion method of 83.27 (µg GAE/g sample). It can be concluded that the two methods used on red pomegranate leaf samples have no significant difference in total phenol content.
Exploration of Secondary Metabolites in Black Pomegranate Peel Extract (Punica granatum L.) as Antioxidants Using DPPH Method Rofiidah, Anisatul; Innaya, Allinha Yusfin; Putri, Nela Vede Rhofa; Maulida, Hilda Jihan; Dewi, Lina Purnama; Al Shofura, Nadya Raisa; Badria, Shoimatul Umi; Faiza, Hilda Wahyu; Hidayah, Ulfa; Ramadhani, Mieta Widya; Rafsanjani, Ramizard; Januarista, Terra; Faisal, Faisal; Ramadhan, Majida
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 2 (2024): JSMARTech Volume 5, No. 2, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.02.46

Abstract

Pomegranate (Punica granatum) is a plant that contains secondary metabolite compounds that can be used as medicinal content. Black pomegranate contains the highest secondary metabolites compared to red pomegranate and white pomegranate, the skin of the pomegranate contains flavonoids, phenols, tannins, and saponins. This study aims to determine the antioxidant activity of black pomegranate peel extract (Punica granatum L.) with the DPPH method. DPPH method is a method to determine the antioxidant activity in the sample by looking at its ability to counteract the free radical compound 2,2-diphenyl-1-picrylhydrazyl. The results of testing the antioxidant activity of black pomegranate skin (Punica granatum L.) produced an IC50 value of 47.51 ppm which can be concluded that black pomegranate skin is classified as very strong. This research is important because there has not been much research on black pomegranate, especially its skin, so there is a need for additional expansion and renewal of the species. In addition, it can be used as the main basis for the advancement of natural materials, especially in the field of health.
Analisis Aktivitas Antioksidan pada Ekstrak Jagung Muda (Zea mays L.) dengan menggunakan Metode DPPH Ma’rifah, Shofia Jannatul; Wahidah, Shinta Nuriyah; Azizah, Nur; Jamilah, Zahrotul; Wati, Dwi Kartika; Arianti, Leny Ardini; Maulidya, Sabrina Dita; Mudzakaroh, Luluk; Safitri, Dewi Astriana; Rafsanjani, Ramizard; Januarista, Terra; Faisal, Faisal; Ramadhan, Majida
Borneo Journal of Biology Education (BJBE) Vol 6, No 2 (2024): Oktober
Publisher : Faculty of Teacher Training and Education Biology Education Departement

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/bjbe.v6i2.5585

Abstract

Pada penelitian ini kami menggunakan jagung muda sebagai pembaharu dari penelitian sebelumnya. Jagung muda merupakan jagung yang masih tergolong mudah (belum berbiji) biasa disebut dengan jagung semi, janggel. Jagung memiliki kandungan fenolik, flavonoid dan tannin yang berguna sebagai antioksidan, yang mampu menghambat dan melindungi radikal bebas. Penelitian ini dilakukan untuk menguji aktivitas ektrak jagung muda menggunakan metode DPPH dengan pelarut etanol. Untuk mengetahui aktivitas antioksidan suatu tanaman, salah satu pengukuran yang paling umum dengan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil). Hasil antioksidan pada ekstrak jagung muda (Zea mays L.) menggunakan metode DPPH dengan spektrofotometri UV-Vis menunjukkan bahwa nilai IC50 pada jagung muda (Zea mays L.) sebesar 257,92. Sehingga dapat disimpulkan bahwa aktivitas antioksidan ekstrak jagung muda (Zea mays L.) termasuk kategori sangat lemah.
Antioxidant Activity Test On Ethanol Extract Of Cassava Leaves (Manihot Esculenta C.) Using DPPH Method Husain, Muhammad Hafizh; Febrianti, Dewi Retno; Salsabillah, Salma; Rolita, Ninda Norma; Amrullah, Kammal Nizam; Bawani, Elen Ayu; Ramadhani, Lailatul; Pratiwi, Lidia Wahyu; Yaqin, Ngainul; Jayanti, Angelina; Rafsanjani, Ramizard; Januarista, Terra; Faisal, Faisal; Ramadhan, Majida
Biopedagogia Vol 6, No 2 (2024): November
Publisher : Program Studi Pendidikan Biologi Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/biopedagogia.v6i2.5533

Abstract

Cassava leaves (Manihot esculenta Crantz) are herbs used in traditional medicine. Cassava leaves contain secondary metabolite chemicals such as vitamin C, flavonoids, tannins, saponins, and triterpenes. Cassava leaves are pharmacologically active as anti-inflammatory, antibacterial, and antioxidants. To identify the sample's active antioxidant components using the DPPH technique. The DPPH method is a method of determining the antioxidant activity of a sample by examining its capacity to neutralize free radicals of the 2,2-diphenyl-1-picrylhydrazyl molecule. The antioxidant activity of quercetin from cassava leaf extract (Manihot esculenta Crantz) yielded an IC50 value of 210.33. The IC50 value shows that cassava leaves (Manihot esculenta Crantz) have modest antioxidant lower. Cassava leaves (Manihot esculenta Crantz) have antioxidant activity of 210.33 g/mL so they are included in the weak category in this study.
Optimization of Analysis Method on Total Phenol Content and Antioxidant Activity in Peel and Seeds of White Pomegranate (Punica Granatum L.) Faisal; Ramadhan, Majida; Rafsanjani, Ramizard; Januarista, Terra
Biotropika: Journal of Tropical Biology Vol. 13 No. 1 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.01.05

Abstract

Pomegranate (Punica granatum L.) peel and seeds are rich in bioactive compounds, particularly phenolics, which contribute significantly to their antioxidant properties. This study aimed to determine the total phenolic content (TPC) and antioxidant activity of white pomegranate peel and seed extracts. TPC was measured using theFolin–Ciocalteu method, while antioxidant activity was assessed via the DPPH radical scavenging assay. Maceration extraction was employed using a 1:1 ethanol–distilled water solvent system. The TPC values of the peel and seed extracts were 99.909 µg/g and 3.424 µg/g samples, respectively, indicating a substantially higher phenolic concentration in the peel. Antioxidant activity, expressed as IC50, was 17.361 ppm for the peel and 26.378 ppm for the seeds, categorizing both as possessing very strong antioxidant potential. The correlation between phenolic content and antioxidant capacity reinforces the role of phenolics as key contributors to antioxidant activity. These results underscore the importance of selecting optimal extraction methods to enhance bioactive compound recovery. The strong antioxidant activity of both peel and seed extracts highlights their potential application as natural antioxidants in the food and pharmaceutical industries.
Antioxidant Activity Test of Bride's Tears (Antigonon leptopus) Leaves Using the DPPH Method Azizah, Nailil Wafiq; Ramadan, Majidah; Faisal, Faisal; Aldina, Devana Rahma; Putri, Ramadina Novia; Anggraini, Nur Laila; Mufarrohah, Sabilatul; Gemilang, Satria; Fitriansyah, Firadika; Hosiyah, Hosiyah; Suci, Nuvia Harini Wulan; Sofiya, Nihlatus; Januarista, Terra; Rafsanjani, Ramizard
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.777-781

Abstract

Bridal tear leaves can be used to treat various health and beauty problems because they contain various active compounds, such as flavonoids, saponins, and tannins. These compounds have antioxidant, anti-inflammatory, and antibacterial properties. This flower contains antioxidants that can help protect the skin from damage caused by free radicals. This research aims to see the effectiveness of antioxidants from bridal tear leaves. The research method involves leaf extraction using 96% ethanol and testing antioxidant activity using the DPPH method. The research results showed that the extract sample from bride's tear leaves was (IC50 – 69.7146 ug/mL). This shows that the bride's tear leaf extract is quite strong in inhibiting free radical reactions.
Co-Authors Al Shofura, Nadya Raisa Aldina, Devana Rahma Amrullah, Kammal Nizam Anggraini, Nur Laila Arianti, Leny Ardini Azizah, Nailil Wafiq Badria, Shoimatul Umi Bawani, Elen Ayu Dewi, Lina Purnama Faisal Faisal Faisal Faiza, Hilda Wahyu Febrianti, Dewi Retno Fitriansyah, Firadika Gemilang, Satria Hidayah, Ulfa Hosiyah, Hosiyah Husain, Muhammad Hafizh Innaya, Allinha Yusfin Jamilah, Zahrotul Jayanti, Angelina Maulida, Hilda Jihan Maulidya, Sabrina Dita Ma’rifah, Shofia Jannatul Mudzakaroh, Luluk Mufarrohah, Sabilatul Nur Azizah Nurul Jadid Mubarokati Pratiwi, Lidia Wahyu Putri, Nela Vede Rhofa Putri, Ramadina Novia Rafsanjani, Ramizard Ramadan, Majidah Ramadhan, Majida Ramadhani, Lailatul Ramadhani, Mieta Widya Rofiidah, Anisatul Rolita, Ninda Norma Safitri, Dewi Astriana Salsabillah, Salma Sofiya, Nihlatus Suci, Nuvia Harini Wulan Wahidah, Shinta Nuriyah Wati, Dwi Kartika Yaqin, Ngainul