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All Journal HAYATI Journal of Biosciences Journal of Bio-molecule Research And Engineering
Rahmafitria, Fistara Lesti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Immunology & microbiology

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Phylogenetic analysis of DENV-1 isolated in Surabaya, Indonesia Rahmafitria, Fistara Lesti; Mahfudhah, Dzikra Nasyaya; Sucipto, Teguh Hari; Herdyastuti, Nuniek; Anggarani, Mirwa Adiprahara; Fauziyah, Shifa; Damayanti, Mamik
Journal of Bio-Molecule Research and Engineering Vol 1 No 2 (2022)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v1i2.41463

Abstract

Dengue virus (DENV1-4) belongs to the Flaviviridae family, which is transmitted by the Aedes mosquito vector and is the main cause of dengue fever and dengue hemorrhagic fever. Since one of the DENV serotypes, DENV1, has become an endemic known to be circulating worldwide, including in Indonesia, it becomes necessary to carry out molecular epidemiological research using phylogenetic analysis with two methods, neighbor-joining (NJ) and UPGMA. This study aims to analyze the DENV-1 relationship and obtain information regarding the differences between those methods, including the level of accuracy. This study used one DENV-1 sequence isolated in Surabaya, aligned with similar sequences on the GenBank. The results showed two comparisons. First, in the NJ method, the DENV-1 sequence samples in Surabaya with branch length 0,000 were similar to the DENV-1 in Malaysia, and Singapore, with branch lengths 0,000; 0,002;. which belong to Genotype 1. The UPGMA method resulted in the DENV-1 sequence in Surabaya with branch length 0,000 were similar to the DENV-1 in Malaysia with with branch length 0,000, which belong to Genotype 1. Second, their level of accuracy, which is in the NJ method, the construction of phylogenetic trees is based on periodic evolutionary times. In contrast, UPGMA assumes that each sequence is found at the same evolutionary time, which makes this method less accurate than the NJ method. We can conclude that the construction and analysis of the phylogenetic tree of the DENV1 sequence isolated in Surabaya have higher similarity and accuracy using the NJ method.
Detection of Vgsc-L1014F Allele Knockdown-resistance Mutation in Male Culex quinquefasciatus Mahfudhah, Dzikra Nasyaya; Rahmafitria, Fistara Lesti; Hari Sucipto, Teguh; Fauziyah, Shifa; Anggarani, Mirwa Adiprahara; Herdyastuti, Nuniek; Naw, Sin War; Witaningrum, Adiana Mutamsari
HAYATI Journal of Biosciences Vol. 32 No. 6 (2025): November 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.6.1400-1406

Abstract

The distribution of Culex quinquefasciatus as a vector for several tropical diseases in Indonesia could affect the transmission of various pathogens, including filariasis, West Nile, encephalitis, and Rift Valley fever. The use of insecticides to control mosquito populations is carried out massively. However, this effort is threatened by an increase in mosquito resistance to insecticides, in this case, pyrethroids that target Voltage-gated sodium channel (VGSC) as a complex protein in mosquito nerve cells that plays a role in the movement of sodium ions, where these compounds can have a knockdown effect and lead to death. The existence of a single nucleotide mutation in this specific codon results in knockdown resistance (kdr) in mosquitoes. This study aims to determine whether there is a kdr mutation in the Vgsc-L1014F target using the latest molecular method ETAS-PCR to type tri-allelic variation at Vgsc-1014 in Cx. quinquefasciatus samples collected in three areas in the cities of Surabaya and Sidoarjo, namely Wonocolo, Sukodono, and Wonoayu. The result showed ten male pool samples (C1-C10) of Cx. quinquefasciatus did not carry mutations or present as homozygous wild type (TTT/TTT), with a DNA band size of 181 bp. Hence, this preliminary study could be extended for further research to build vector control monitoring programs.
Co-Authors Adiana Mutamsari Witaningrum DAMAYANTI, MAMIK Hari Sucipto, Teguh Mahfudhah, Dzikra Nasyaya Mirwa Adiprahara Anggarani Naw, Sin War Nuniek Herdyastuti Shifa Fauziyah Teguh Hari Sucipto, Teguh Hari