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All Journal HAYATI Journal of Biosciences Vegetalika Jurnal Pijar MIPA Journal of Bio-molecule Research And Engineering
Mahfudhah, Dzikra Nasyaya
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Immunology & microbiology Mathematics Physics

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Phylogenetic analysis of DENV-1 isolated in Surabaya, Indonesia Rahmafitria, Fistara Lesti; Mahfudhah, Dzikra Nasyaya; Sucipto, Teguh Hari; Herdyastuti, Nuniek; Anggarani, Mirwa Adiprahara; Fauziyah, Shifa; Damayanti, Mamik
Journal of Bio-Molecule Research and Engineering Vol 1 No 2 (2022)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v1i2.41463

Abstract

Dengue virus (DENV1-4) belongs to the Flaviviridae family, which is transmitted by the Aedes mosquito vector and is the main cause of dengue fever and dengue hemorrhagic fever. Since one of the DENV serotypes, DENV1, has become an endemic known to be circulating worldwide, including in Indonesia, it becomes necessary to carry out molecular epidemiological research using phylogenetic analysis with two methods, neighbor-joining (NJ) and UPGMA. This study aims to analyze the DENV-1 relationship and obtain information regarding the differences between those methods, including the level of accuracy. This study used one DENV-1 sequence isolated in Surabaya, aligned with similar sequences on the GenBank. The results showed two comparisons. First, in the NJ method, the DENV-1 sequence samples in Surabaya with branch length 0,000 were similar to the DENV-1 in Malaysia, and Singapore, with branch lengths 0,000; 0,002;. which belong to Genotype 1. The UPGMA method resulted in the DENV-1 sequence in Surabaya with branch length 0,000 were similar to the DENV-1 in Malaysia with with branch length 0,000, which belong to Genotype 1. Second, their level of accuracy, which is in the NJ method, the construction of phylogenetic trees is based on periodic evolutionary times. In contrast, UPGMA assumes that each sequence is found at the same evolutionary time, which makes this method less accurate than the NJ method. We can conclude that the construction and analysis of the phylogenetic tree of the DENV1 sequence isolated in Surabaya have higher similarity and accuracy using the NJ method.
Pengaruh Konsentrasi Ekstrak Bawang Bombay Merah (Allium cepa L.) terhadap Total Fenolik dan Aktivitas Antioksidan Tanaman Telang (Clitoria ternatea L.) Anggarani, Mirwa Adiprahara; Mahfudhah, Dzikra Nasyaya
Vegetalika Vol 14, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.101541

Abstract

Pemanfaatan Zat Pengatur Tumbuh (ZPT) sebagai langkah optimalisasi budidaya tanaman bunga telang (Clitoria ternatea L.) yang khasiatnya telah dikenal massif di tengah-tengah masyarakat diperlukan untuk semakin meningkatkan kualitas dan kuantitas bunga telang, sehingga nilai ekonomi bunga telang juga akan semakin bertambah, utamanya dalam konteks budidaya tanaman dan pengembangan industri pertanian. Penggunaan ZPT alami yang lebih ramah lingkungan dan harga terjangkau jika dibandingkan dengan ZPT sintetik yang berpotensi mencemari lingkungan dalam jangka panjang menjadi salah satu opsi yang tepat. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi ZPT alami bawang bombay merah (Allium cepa L.) terhadap total fenolik dan aktivitas antioksidan bunga telang. Budidaya bunga telang dilaksanakan di Desa Burneh, Bangkalan Madura pada bulan Juni 2023 sampai Oktober 2023. Metode penelitian ini berbasis kuantitatif, dengan rancangan percobaan menggunakan Rancangan Acak Kelompok (RAK) satu faktor, yakni variasi konsentrasi ZPT terdiri dari 0% sebagai kontrol, 1%, 3%, 5%, 7%, dan 10%, dimana masing-masing konsentrasi direplikasi 4 kali. Hasil penelitian menunjukkan bahwa ZPT alami berpengaruh positif terhadap total fenolik dan aktivitas antioksidan bunga telang, dimana hasil tertinggi diperoleh pada variasi konsentrasi ZPT 10% dengan total fenolik sebesar 21,570 mgGAE/g ekstrak dan nilai IC50 sebesar 51,04 ppm yang termasuk kategori antioksidan kuat.
Detection of Vgsc-L1014F Allele Knockdown-resistance Mutation in Male Culex quinquefasciatus Mahfudhah, Dzikra Nasyaya; Rahmafitria, Fistara Lesti; Hari Sucipto, Teguh; Fauziyah, Shifa; Anggarani, Mirwa Adiprahara; Herdyastuti, Nuniek; Naw, Sin War; Witaningrum, Adiana Mutamsari
HAYATI Journal of Biosciences Vol. 32 No. 6 (2025): November 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.6.1400-1406

Abstract

The distribution of Culex quinquefasciatus as a vector for several tropical diseases in Indonesia could affect the transmission of various pathogens, including filariasis, West Nile, encephalitis, and Rift Valley fever. The use of insecticides to control mosquito populations is carried out massively. However, this effort is threatened by an increase in mosquito resistance to insecticides, in this case, pyrethroids that target Voltage-gated sodium channel (VGSC) as a complex protein in mosquito nerve cells that plays a role in the movement of sodium ions, where these compounds can have a knockdown effect and lead to death. The existence of a single nucleotide mutation in this specific codon results in knockdown resistance (kdr) in mosquitoes. This study aims to determine whether there is a kdr mutation in the Vgsc-L1014F target using the latest molecular method ETAS-PCR to type tri-allelic variation at Vgsc-1014 in Cx. quinquefasciatus samples collected in three areas in the cities of Surabaya and Sidoarjo, namely Wonocolo, Sukodono, and Wonoayu. The result showed ten male pool samples (C1-C10) of Cx. quinquefasciatus did not carry mutations or present as homozygous wild type (TTT/TTT), with a DNA band size of 181 bp. Hence, this preliminary study could be extended for further research to build vector control monitoring programs.
The Effect of Natural Plant Growth Regulator Concentration of Indian Red Onions on the Growth of Euphorbia (Euphorbia geroldii) Flowers Mahfudhah, Dzikra Nasyaya; Anggarani, Mirwa Adiprahara
Jurnal Pijar Mipa Vol. 19 No. 2 (2024): March 2024
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v19i2.6363

Abstract

The euphorbia flower (euphorbia geroldii), widely used by the public as an ornamental plant because of its visual beauty, has an aesthetic function and health benefits that still need to be researched. The euphorbia genus has antimicrobial, antioxidant, and antidiabetic activity, which comes from its abundant terpenoid compounds, flavonoids, and other phytochemical compounds. Due to these high benefits, optimization of cultivation is carried out by adding phytohormones, better known as Growth Regulatory Regulator (PGR), further to increase the quality and quantity of euphorbia flowers so that the economic value will also increase in the realm of the agricultural industry. This research aims to analyze the effect of the natural PGR concentration of red onions (Allium cepa L.) on the growth of euphorbia flowers, including the parameters of the number of flowers on one tree, the time the buds appear, and the duration of flower survival. The research method used was quantitative experimental using a one-factor Randomized Block Design (RBD), namely testing the effect of several variations in PGR concentration (0%, 1%, 3%, 5%, 7%, and 10%) and statistically analyzed using SPSS. The results of the research show that all variations in PGR concentration have a positive effect on all parameters, where the PGR concentration of 10% indicates the highest increase, including the average number of flowers on one tree, which is 13 flowers, the average time for buds to appear is 2.5 times in one week. The average duration of flower survival is 139.5 hours. It can be concluded that the natural PGR of red onions has a positive effect on all parameters, with the highest results shown at a concentration variation of 10%.
Co-Authors Adiana Mutamsari Witaningrum DAMAYANTI, MAMIK Mirwa Adiprahara Anggarani Naw, Sin War Nuniek Herdyastuti Rahmafitria, Fistara Lesti Shifa Fauziyah Teguh Hari Sucipto, Teguh Hari