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All Journal Journal of Bio-molecule Research And Engineering
Rahmawati, Hanif Nur
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Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology

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Screening and Identification of Nitrogen-Fixing Bacteria from Rhizosphere of Mangrove Jenu Tuban Salsabila, Syarifah; Rahmawati, Hanif Nur; Fatimah, Fatimah
Journal of Bio-Molecule Research and Engineering Vol 1 No 2 (2022)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v1i2.45225

Abstract

this study aims to determine nitrogen-fixing bacteria isolates from Jenu mangrove soil, their macroscopic and microscopic characteristics, and species based on the 16S rRNA gene. nitrogen-fixing bacteria were filtered qualitatively using a selective nitrogen-free bromothymol (NFB) medium. screening results showed that six bacterial isolates from Jenu Tuban mangrove soil had potential as nitrogen -fixing bacteria, namely 1,8,10,15, and 16. the six nitrogen-fixing bacteria had varied microscopic characteristics and were gram-positive. based on the identification of the 16S rRNA, it was found that the six isolates were detected as a Bacillus cereus group with 99% query cover and 99.58% identitiy; Bacillus toyonensis with 99% query cover and 99.79% identity; Bacillus altitudinis with 99% query cover and 99.72% identity; Bacillus megaterium with 99% query cover and 99.79% identity; and Bacillus aerius with 98% query cover and 99.86% identitiy.
Identification of Saprophytic Mold Isolated from Mangrove Soil: A Review Rahmawati, Hanif Nur; salsabila, Syarifah salsabila; Rahayuningtras, Nazil Dwi; Nurhariyati, Tri; Fatimah
Journal of Bio-Molecule Research and Engineering Vol 2 No 1 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i1.50546

Abstract

Saprophytic mold is one type of mold that has the ability to survive in extreme conditions, such as in mangrove soil. Saprophytic fungi act as the main organism in the process of decomposition of organic matter and energy flow in the mangrove area. Several researchers successfully identified 102 species and 33 genera of saprophytic fungi. Aspergillus is known to be the dominant mold on mangrove soils because of its characteristics, such as high sporulation and spore spread and its ability to withstand extreme environmental conditions. The use of saprophytic molds in biofertilizer manufacturing due to saprophytic molds can produce cellulase, xylinase, and ligninase enzymes to decompose organic matter and produce toxin compounds for pathogens in plants. The presence of mold species in mangrove soil is influenced by several factors, including the temperature level, season, the salinity of mangrove waters, soil pH, and oxygen.
Studies of Yeasts Isolated from Soil as Cellulose Decomposers and Phosphate Solvents Putra, Gading Gunawan; salsabila, Syarifah salsabila; Rahmawati, Hanif Nur; fatimah
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.65587

Abstract

The identification of CMCase (Carboxymethylcellulase) and PMEase (Phosphomonoesterase) enzyme activities from yeast genera that produce cellulase and phosphatase is crucial for identifying potential genera that could aid in the development of biofertilizers, serving as an environmentally friendly alternative to chemical fertilizers. This study is based on a review of articles and journals for data collection. The review revealed that the yeast genus Rhodosporidium (specifically Rhodosporidium paludigenum Y08RA29) is a promising cellulolytic yeast, with CMCase activity approaching 0.500 units. Meanwhile, the potential phosphate-solubilizing yeast genus is Candida (Candida sp. 3), with PMEase activity ranging from 0.05 to 0.06 units.
Co-Authors Fatimah fatimah Fatimah Putra, Gading Gunawan Rahayuningtras, Nazil Dwi Salsabila, Syarifah salsabila, Syarifah salsabila Tri Nurhariyati