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All Journal HAYATI Journal of Biosciences IPTEK Journal of Proceedings Series Jurnal Kimia Riset Darmabakti Cendekia: Journal of Community Service and Engagements Jurnal Layanan Masyarakat (Journal of Public Service) Jurnal Riset Biologi dan Aplikasinya Jurnal Pengabdian kepada Masyarakat Nusantara Berkala Penelitian Hayati Prosiding Konferensi Nasional PKM-CSR Journal of Bio-molecule Research And Engineering

Tri Nurhariyati

Department Of Biology, Faculty Of Science And Technology, Airlangga University, Surabaya

Author-ID : 334728

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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The Alternative Use Of Water Hyacinth and Reed As Attached Growth Of Microbial In Waste Water Treatment Hery Purnobasuki; Nur Indradewi Oktavitri; Tri Nurhariyati; B. Saadah; Santini Ika Rafsanjani
IPTEK Journal of Proceedings Series Vol 1, No 1 (2014): International Seminar on Applied Technology, Science, and Arts (APTECS) 2013
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j23546026.y2014i1.227

Aquatic plants have ability to decrease of waste water contaminant. The Water Hyacinth (Eichornia crassipes) and Reed (Imperata cylindrical) are aquatic plants which have ability to reduce concentration of BOD, TSS, COD, Total Nitrate and Total Phosphate in wetland. The other side, it can be used for media support in anaerobic treatment. Because of their abilities, there is some possibilities that Water Hyacinth and Reed can be used as attached growth of microorganisms. The aims of this study was to investigate the potential of water hyacinth and reed as attached media of microbe growth. Part of the plants are used is the trunk part. A slices of trunk is 1 cm. The analysis were conducted for 14 days with ratio 60% waste water and 40% air of reactor. Results of this study for water hyacinth that TSS decrease up to 71%. COD and Total Phosphate decrease 60%, Total Nitrate decrease 85%. While, for reed, TSS decrease up to 41%. COD decrease 56%, Total Phosphate decrease 41%. Total Nitrate decrease 10%. In conclusion, both of them had a potential as attached media of microbe growth. But, Water Hyacinth was better as media support than reed.

ISOLATION OF BIOACTIVE COMPOUNDS FROM DICRANACEAE MOSSES Junairiah Junairiah; Tri Nurhariyati; Ni'matuzahroh Ni'matuzahroh; Lilis Sulistyorini
Jurnal Kimia Riset Vol. 1 No. 2 (2016): Desember
Publisher : Universitas Airlangga

ABSTRACT Dicranoloma reflexum and Dicranella coarctata are mosses from Dicranaceae family. This study was purposed to identify bioactive compounds contained from both species. Dicranoloma reflexum and Dicranella coarctata collected form Cangar forest, Batu, East Java. Mosses was rinsed, dried and crushed into powder. Extraction was performed using maceration method with n-hexane, acetic acid, and methanol solvent. Compounds obtained then identified using Gass Chromatography Mass Spectra. Result showed that n-hexane, acetic ethyl, and methanol extract of Dicranoloma reflexum contained 61, 16, and 58 compounds respectively. Main component of each extract was 1-octadecene, phenol, and 9-octadecanoic acid. N-hexane, acetic ethyl, and methanol extract of Dicranella coarctata contained 5, 38, and 23 compounds respectively. Main component of each extract was thiosulphuric acid, E-15 heptedecenal, and n-hexadecanoic acid.Key words : Dicranaceae, bioactive compounds

INNOVATION OF MEDICINE PLANTS PROPAGATION WITH VERTICULTURE TECHNIQUES IN EFFORTS TO OVERCOME LAND DYSFUNCTIONAL IN EAST SURABAYA AREA Junairiah Junairiah; Tri Nurhariyati; Listijani Suhargo
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 2 No. 2 (2020): DECEMBER 2020
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Background: Keputih Village is located in eastern Surabaya. The boundary of this village is the river. Areas on the river banks are overgrown with wild plants and poorly maintained. One way to overcome the dysfunctional land around the river is by cultivating medicinal plants with verticulture techniques. Purpose: The implementation of this program is to overcome the dysfunctional land around the Keputih Village’s river by cultivating medicinal plants in verticulture techniques and provide training in the manufacture of herbal products from the cultivation of medicinal plants and provide financial management training to the local residents. Methods: This activity was carried out in 4 stages. Phase I was training in making pots and verticulture shelves as well as planting medicinal plants, Phase II was mentoring in the field, Phase III was training on making herbal products from plants and training on simple bookkeeping, Phase IV was product display. Results: Based on the results of this training showed that the knowledge of the participants related to the training material has increased which shown from the results of the posttest with average of 79,31 and product display. Conclusion: In this activity, beside gaining knowledge, participants can apply this knowledge to increase family income.

PELATIHAN PEMBUATAN PUPUK ORGANIK CAIR PADA KELOMPOK TANI DI KABUPATEN PROBOLINGGO Tini Surtiningsih; Fatimah Fatimah; Ni’matuzahroh Ni’matuzahroh; Agus Supriyanto; Tri Nurhariyati
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 1 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

This community service aims to apply the method of making and liquid organic fertilizer and improving the skills of farmers in making liquid organic fertilizer. In addition to reducing the dependence of farmer groups on inorganic fertilizers and utilizing the results of sugarcane waste, namely molasses and microbes as a formula in the manufacture of liquid organic fertilizer. The method that was carried out was to increase insight into liquid organic fertilizer, and how to make it. The results of the socialization of liquid organic fertilizers showed that the insights of farmer groups increased by an average of 77% through the pretest and post test values during socialization. Based on the evaluation shows that the farmer group has been able to make liquid organic fertilizer independently. The socialization of making liquid organic fertilizer provides additional insight for farmer groups on the use of liquid organic fertilizer for agriculture.AbstrakPengabdian kepada masyarakat ini bertujuan untuk menerapkan cara pembuatan dan pupuk organik cair dan meningkatkan keterampilan petani dalam membuat pupuk organik cair. Selain itu untuk mengurangi ketergantungan kelompok tani terhadap pupuk anorganik dan memanfaatkan hasil limbah tebu yaitu molase dan mikroba sebagai formula dalam pembuatan pupuk organik cair. Metode yang dilakukan adalah peningkatan wawasan mengenai pupuk organik cair, dan cara pembuatannya. Hasil sosialisasi pupuk organik cairmenunjukkan bahwa wawasan kelompok tani meningkat rata-rata sebesar 77% melalui nilai pretest dan post test saat sosialisasi. Berdasarkan evaluasi menunjukkan bahwa kelompok tani telah mampu membuat pupuk organik cair secara mandiri. Sosialisasi pembuatan pupuk organik cair memberikan tambahan wawasan bagi kelompok tani terhadap pemanfaatan pupuk organik cair bagi pertanian.

PELATIHAN PENGOLAHAN LIMBAH TOILET MENGGUNAKAN FORMULA MIKROBA DI PERUMAHAN TENGGILIS SURABAYA Fatimah Fatimah; Tini Surtiningsih; Agus Supriyanto; Nima’tuzahroh Nima’tuzahroh; Salamun Salamun; Tri Nurhariyati
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 1 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Blockage of septic tanks is one of the problems in the community related to the discharge of toilet waste produced. The use of synthetic toilet cleaners that are not environmentally friendly is one of the factors that can inhibit the process of decomposing organic waste. The existence of microbial killer ingredients in toilet cleaning materials that come into the septic tank will actually inhibit the growth of essential microbes that play a role in the perfection of the decomposition process. Lack of understanding of the community related to microbial processes in the processing of toilet waste is one of the factors inability of the community to overcome these problems. This community service aims to disseminate information related to the processing of organic waste, the role of microbes in the processing of toilet waste, how to make microbial formulas that play a role in the processing of toilet waste, as well as efforts to increase the creativity of the community to process their own waste to improve environmental sanitation and improve community welfare. The results of the training showed that the average pretest and post test scores of the cadres of Tenggilis Surabaya village experienced a significant increase in value with a mean value of 37.78 (pretest) to 62.22 (post-test). Toilet waste processing cadres have been able to make biotoilet products that can be used to process limbat toilets in their homes. The informationand insights they receive can be passed on to the community from each cadre.AbstrakPenyumbatan septic tank merupakan salah satu permasalahan di masyarakat terkait dengan buangan limbah toilet yang dihasilkan. Penggunaan pembersih toilet yang berbahan sintetik yang tidak ramah lingkungan merupakan salah satu faktor yang dapat menghambat proses penguraian limbah organik. Keberadaan bahan pembunuh mikroba dalam bahan-bahan pembersih toilet yang ikut masuk ke dalam septik tank justru akan menghambat pertumbuhan mikroba esensial yang berperan dalam kesempurnaan proses dekomposisi. Kurangnya pemahaman masyarakat terkait dengan proses mikroba dalam pengolahan limbah toilet merupakan salah satu faktor ketidakmampuan masyarakat dalam mengatasi permasalahan tersebut. Pengabdian masyarakat ini bertujuanuntuk mensosialisasikan informasi terkait proses pengolahan limbah organik, peran mikroba dalam pengolahan limbah toilet, cara membuat formula mikroba yang berperan dalam pengolahan limbah toilet, serta upaya meningkatkan kreativitas masyarakat untuk mengolah limbahnya sendiri guna meningkatkan sanitasi lingkungan dan meningkatkan kesejahteraan masyarakat. Hasil pelatihan menunjukkan bahwa rerata nilai pretest dan post test kader kelurahan Tenggilis Surabaya mengalami peningkatan nilai yang cukup signifi kan dengan rerata nilai dari 37,78 (pretest) menjadi 62,22 (post test). Kader pengolahan limbah toilet telah mampu membuat produk biotoilet yang dapat dimanfaatkan untuk mengolah limbat toilet di rumah masing-masing. Informasi dan wawasan yang mereka terima dapat di teruskan kepada komunitas asal masing-masing kader.

BIOFERTILISASI BAKTERI Rhizobium PADA TANAMAN KEDELAI (Glycine Max (L) Merr.) Tini Surtiningsih; Farida; Tri Nurhariyati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009
Publisher : The East Java Biological Society

The aim of this research want to know the influence of the addition Rhizobium bacteria species, dose and combination both of them, on growth and production of soybean plant (Glycine max (L) Merr.). The experimental design of this research was factorial design 4×2, 4 species of Rhizobium are R1 = Rhizobium japonicum, R2 = R. phaseoli, R3 = R. leguminosarum, R4 = mixture of R1, R2 and R3, and 2 dose of inoculan Rhizobium (D1 = 5 m/plant, and D2 = 10 ml/plant) with 1010 sel bacteria/ml and 5 replications. Independent variable is species of Rhizobium, dose of inoculan Rhizobium and combination both of them. Dependent variable is dry matter, weight of nodules and dry weight of seeds. The harvest data was analyzed by Kruskal-Wallis Test using 5% level (a = 0.05) followed by Mann- Whitney Test. The result of this research show that species of Rhizobium, dose of inoculan Rhizobium and combination both of them present insignificant result (a > 0.05) on soybean growth and production, but the mixture of Rhizobium species with high level dose of bacteria, present better result than single species with low dose of bacteria.

STUDI KINETIKA PRODUKSI BIOSURFAKTAN Bacillus subtilis 3KP PADA SUBSTRAT MOLASE Ni’matuzahroh; Nur Hidayatul Alami; A. Faiz Khudlari T.; Fatimah; Tri Nurhariyati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/281

Bacillus subtilis 3KP was a bacteria that can produce biosurfactant, when it was grown on a molasses substrate. This research was aimed to know the kinetic type of biosurfactant production, so it can be known the efficiency of molasses and the optimal culture condition to produce biosurfactant. B. subtilis 3KP was grown on mineral salt medium that had been already added with various molasses concentrations (1, 2, 3, and 4 g/l). The culture was incubated until 7 days on a shaker at 27° C and 120 rpm. The measurement of bacterial growth (CFU/ml), pH culture changing, and biosurfactant production which was indicated from emulsification activity and crude biosurfactant weight was determined at various incubation periods (0, 1, 2, 3, 4, 5, 6 and 7 days). Emulsifier activity (E1h) was measured by adding 1 ml of kerosene to 1 ml of biosurfactant supernatant. The measurement was made after 1 hour. The E1h index was given as percentage of height of emulsified layer (cm) divided by total height of the liquid column (cm). Crude biosurfactant was got from ammonium sulfate precipitation, then was dried and was determined in (g/l culture). The data of growth and biosurfactant production were analysed descriptively to know differentiation of biosurfactant production type of B. subtilis 3 KP in that different treatment. The examination of kinetics parameters such as product yield (Yp/x) and specific rate of culture (μ) were taken into account to evaluate a high efficient production process. The results of this research indicated that biosurfactant of B. subtilis 3KP was begun produced at exponential phase and was optimal at the end of exponential phase (production under growth-limiting conditions). Molasses concentration of 3% and 4 days incubation gave an optimal biosurfactant production, so it can be became the exact combination alternative to scale up biosurfactant of B. subtilis 3KP.

BIODEGRADASI MINYAK OLEH Rhodotorula dan Candida HASIL ISOLASI DARI PELABUHAN TANJUNG PERAK SURABAYA Tri Nurhariyati; Ni’matuzahroh Ni’matuzahroh; Tini Surtiningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 1 (2006): December 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/394

A research about isolation and capability of isolat yeast from Tanjung Perak Harbor, Surabaya in degrading kerosen, gas oil ,and lubricant were conducted. This research were done to know the influence of Rhodotorula, Candida and Mix of Rhodotorula and Candida; the influence of oil type and interaction of both (between yeast and oil) in decreasing oil weight. Research design was laboratory experimental and using the factorial 4 × 3 with five replication. The data were analyzed by two-way ANOVA (p: 0.05) and followed by t test. The result of the biodegradation test showed that the kind of yeast, the kind of oil weight and interaction of both influence in decreasing of oil weight. The most decreasing of oil weight obtained by mix of Rhodotorula and Candida (82.55%), kerosen (66.59%) and combination mix of yeast (Rhodotorula and Candida) and kerosen (89.32%).

KEANEKARAGAMAN KHAMIR PENDEGRADASI MINYAK HASIL ISOLASI DARI PELABUHAN TANJUNG PERAK SURABAYA Tri Nurhariyati; Ni’matuzahroh Ni’matuzahroh; Tini Surtiningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 2 (2004): June 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/427

The aims of this research was to obtain diversity of hydrocarbonoclastic yeast isolated from Tanjung perak harbor Surabaya. Exploration of yeast was conducted by isolation and identification of isolated yeast. Identification of yeast based on characteristics of colonies, cell shape, and biochemical tests. From this research, it was obtained 9 hydrocarbonoclastic yeasts. They were 8 generas: Rhodotorula, Candida, Geotrichum, Torulopsis, Trichosporon, Cryptococcus, Debaryomyces, and Saccharomyces.

PERSISTENSI TOKSISITAS BIOINSEKTISIDA SPHEREFIXTM PADA BEBERAPA TIPE TEMPAT PERINDUKAN NYAMUK Aedesaegypti L. Salamun Salamun; S A Husein; Rosmanida Rosmanida; Dwi Winarni; Tri Nurhariyati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 6 No 1 (2000): December 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/480

SpherefixTM bioinsecticide is microbial agent of Bacillus sphaericus H-5a5b (VCRC B24) showing high potency for vector control. The bioinsecticide specific to target insect, and do not produce any adverse environmental impact, so the bioinsecticide would be very promising agent for vector control, especially vector of dengue haemorrhagic fever in Indonesia. The present studies aimed at observing the toxicity persistence of SpherefixTM on the larvae Aedesaegypti L. in some types of water container, and recycling potencies in the breeding places of A.aegypti. Two steps of the studies were carried out under laboratory conditions. Firststeps were reared mosquitos in the laboratory to supply larvae of Aedes aegypti. Second step testing of SpherefixTM toxicity persistence in the cemented, clay, and plastic containiers. The bioinsecticides were preparated by Vector Control Research Centre (VCRC), India. The toxicity persistence of SpherefixTM on the larvae of A. aegypti L. in some types of water container were decided by probit analysis. The toxicity persistence tests were carried out by time series observation on the day 1, 6, 12,,,, and 120. The result showed that difference toxicity persistence of the SpherefixTM in the breeding places of A. aegypti. The cemented container was found to have longest of toxicity persistence of the SpherefixTM followed by the plastic container, and the clay container ha the shortest duration toxicity persistence. The higher concentration of the boinsecticides, will result in higher toxicity persistence. The SpherefixTM showed recycling potency in the breeding places of A. aegypti. Percent motility of the larvae dropped sharply after day 50, and larvae mortality under 5% after day 78.

Co-Authors A. Faiz Khudlari T. Affandi, Moch Agus Supriyanto Agus Supriyanto Asrifah Ramly , Zakia Asrifah Ramly, Zakia B. Saadah Bambang Irawan /E21207042 Dwi Winarni Farida Fatimah Fatimah Fatimah fatimah Fatimah Hery Purnobasuki Izzatul Millah, Annida Junairiah Junairiah Lailatul Ayu Fadilah, Risky Lilis Sulistyorini Listijani Suhargo, Listijani Nafidiastri, Farah Aisyah Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh, Ni'matuzahroh Nima’tuzahroh Nima’tuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Nur Hidayatul Alami Nur Indradewi Oktavitri Rahayuningtras, Nazil Dwi Rahmawati, Hanif Nur Rini Nur Izza Zuhri, Afifa Rosmanida S A Husein Saikhu Ahmad Husen Salamun Salamun Salamun Salamun Salsabila, Syarifah salsabila, Syarifah salsabila Santini Ika Rafsanjani Sugiarti , Tipuk Tini Surtiningsih Tsana, Izdihar Watamtin Widhiya, Eva Y Sri Wulan Manuhara

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