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All Journal Jurnal Teknologi Dan Industri Pangan Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Mahara, Fenny Amilia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering

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The Manipulation of Folate Biosynthesis in Lactic Acid Bacteria with a Folate Analog and Enhancers Mahara, Fenny Amilia; Nuraida, Lilis; Nurjanah, Siti
Jurnal Teknologi dan Industri Pangan Vol. 35 No. 2 (2024): Jurnal Teknologi dan Industri Pangan
Publisher : Perhimpunan Ahli Teknologi Pangan Indonesia bekerjasama dengan Departemen Ilmu dan Teknologi Pangan, IPB University Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2024.35.2.162

Abstract

Folate biosynthesis in Lacticaseibacillus rhamnosus R23 isolate of breast milk and Limosilactobacillus fermentum JK13 isolate of kefir granules was manipulated by stressing them with a folate analog (methotrexate) to increase extracellular folate production. Extracellular folate production was further enhanced in isolate R23 (as a folate-efficient probiotic) by adding various enhancer compounds (PABA, glutamate, combination PABA-glutamate, CaCl2, and ascorbic acid) in a folate-free medium. Extracellular folate analysis was done using a microbiological assay that quantified all forms of folate in the samples. Both isolates could grow in folate-free medium containing methotrexate (2.5 mg/L); however, random mutant colonies of both had no increase in extracellular folate production. The resistance mechanism against methotrexate did not trigger excessive extracellular folate production but caused bacterial filamentation. Adding various enhancer compounds also did not significantly increase the extracellular folate production of isolate R23, probably due to the inadequate concentration of the compounds. This study's results indicate that stress exposure to methotrexate seems to be ineffective to increase the extracellular folate production of isolates R23 and JK13. The formation of bacterial filaments in response to stress exposure to methotrexate is possibly a new mechanism that has not been previously reported regarding the mechanism of methotrexate resistance by lactic acid bacteria. This study requires further investigation primarily evaluating intracellular folate concentrations and finding optimum concentrations of different folate biosynthesis enhancer compounds.
Viability of Encapsulated Bacteria using Gel Particle and Foam-Mat Drying with k-carrageenan/Chitosan and i-carrageenan/Chitosan Setijawati, Dwi; Jaziri, Abdul Aziz; Awang, Mohd Azrie Bin; Firdaus, Muhamad; Budiutami, Aryanti Diah Ayu; Alifiani, Alifiani; Mahara, Fenny Amilia; Islamy, R Adharyan
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 20, No 2 (2025): August 2025
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.860

Abstract

The study aimed to determine the effect of using a mixture of kappa-carrageenan/Chitosan and Iota carrageenan/Chitosan as encapsulating materials for Lactobacillus acidophilus and Bifidobacterium bifidum on viability, encapsulation yield, moisture content and aw of microcapsules. The research method is an experimental laboratory design, ANOVA, LSD using the Excel program. The results showed that using chitosan with a DD of 83.5% in the i-carrageenan/Chitosan mixture gave the highest viability of Bifidobacterium bifidum. The combination of i-carrageenan/chitosan coating ingredients with a concentration ratio of 0.5%:1.5% produced probiotic viability (L aci + B bifi) of 6.56 log CFU/g, while probiotic viability (B bifi) was the highest with a viability 6.70 log CFU/g. The highest probiotic encapsulation yield (L aci+B bifi) was 72.85%. The water content of the microcapsules (L aci+B bifi) was 8.96%, and the aw value was 0.63. It is recommended to carry out further testing using a processing factor for its viability.  
Co-Authors Abdul Aziz Jaziri, Abdul Aziz Alifiani, Alifiani Awang, Mohd Azrie Bin Budiutami, Aryanti Diah Ayu Dwi Setijawati Islamy, R Adharyan Lilis Nuraida Muhamad Firdaus Siti Nurjanah