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All Journal Sains Natural: Journal of Biology and Chemistry IJCR (Indonesian Journal of Chemical Research) JURNAL PENGABDIAN PENDIDIKAN MASYARAKAT (JPPM)
Landi Pote, Lodowik
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Public Health Social Sciences Other

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Pelatihan PELATIHAN PELAJAR KELAS XII IPA SMA MENELITI MELALUI SAYEMBARA RISET OBAT TRADISIONAL Tukan, Gerardus Diri; Markus Taek, Maximus; Latumakulita, Gertreda; Nadut, Anggelinus; Landi Pote, Lodowik; Dewi Q. M. Bulin, Christiani; Risan Funan Lalong, Paulus
Jurnal Pengabdian Pendidikan Masyarakat (JPPM) Vol 6 No 1 (2025): Jurnal Pengabdian Pendidikan Masyarakat (JPPM), Vol 6 No 1 (Maret 2025)
Publisher : LPPM UNIVERSITAS MUHAMMADIYAH MUARA BUNGO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52060/jppm.v6i1.2392

Abstract

High school students in Indonesia, in today's development, have a final assignment before completing their senior secondary education, namely conducting scientific research, writing up research results and presenting them. General conditions show that female students still have difficulty conducting research which includes research ideas or topics, steps for conducting research and writing research results. In response to this condition, research training was created for class The method used is competition. Female students are invited to conduct research, collect data and analyze data in their respective places of residence during the holiday period. As a result of the activities obtained, as many as 35 female students took part in the competition. It appears that the participants dug up information, conducted interviews with sources, namely parents in the villages, then wrote down the results of their studies and pictures of traditional medicinal ingredients.  From the results of the competition, a scientific seminar was held with the theme; Traditional Medicine of the NTT Community; Chemical Content and Activity. The top four competition participants were selected as presenters, and discussed by Natural Materials Chemistry experts in the scientific seminar forum. It was concluded that through research competitions and seminars, it had become a means to train female students to carry out scientific research in their living environment, related to traditional medicine and present their research work.
Isolasi dan Karakterisasi Senyawa Metabolit Sekunder Dari Kulit Batang Tumbuhan Niko (Grewia koordersiana Burret) Klotilde Manek, Marliana; Landi Pote, Lodowik; Nadut, Anggelinus
INDONESIAN JOURNAL OF CHEMICAL RESEARCH Vol. 9 No. 2 (2024): Volume 9, ISSUE 2,2024
Publisher : Chemistry Department, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijcr.vol9.iss2.art7

Abstract

Isolation and characterization of secondary metabolite compounds from the bark of the Niko plant (G. koordersiana Burret) aims to determine the type of secondary metabolite compounds and the characteristics of secondary metabolite compounds. The methods used are extraction, chromatography and spectrophotometry. A total of 500 g of Niko bark powder, macerated with methanol solvent, obtained a thick extract with a yield of 52.09%. The results of phytochemical tests showed that the methanol extract contained alkaloids, flavonoids, terpenoids and saponins. The results of fractionation by column chromatography using methanol eluent: ethyl acetate (1:3) obtained 20 fractions. The results of TLC for each fraction obtained 4 fractions with a single spot, namely fractions 11, 12, 15 and 16, where fractions 11 and 16 had Rf 0.3 and fractions 12 and 15 had Rf 0.7. Fraction A is fraction 11 and 16 with single spot concentrated and obtained extract of 2.09 grams (8.93%) and fraction B is fraction 12 and 15 obtained extract of 2.74 grams (11.53%). The results of characterization by UV-Vis spectrophotometry for fraction A obtained two absorption bands at wavelengths of 280 and 235 nm. Fraction B obtained two absorption bands at wavelengths of 280 and 245 nm. The results of the characterization using Infrared spectroscopy of methanol extract of Niko bark (G. koordersiana Burret) showed the presence of -OH groups at wave numbers 3383.14 cm-1 and aliphatic C-H groups (stretching) at wave numbers 2983.88 cm-1, carbonyl C=O groups at wave numbers 1741.72 cm-1, aliphatic C-H groups (bending) and alcohol C-O groups at wavelengths 1244.09, 1045.42 and 1033.85 cm-1. Based on the results of the UV-Vis and IR spectra, both isolates contain the same compounds.
Skrining Fitokimia dan Karakterisasi Senyawa Metabolit Sekunder Ekstrak Daun Tumbuhan Niko (Grewia koordersiana Burret) Landi Pote, Lodowik; Elisabeth Ipih, Hapat; Maximus M., Taek
INDONESIAN JOURNAL OF CHEMICAL RESEARCH Vol. 10 No. 1 (2025): Volume 10, ISSUE 1, 2025
Publisher : Chemistry Department, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijcr.vol10.iss1.art1

Abstract

Plants contain secondary metabolite compounds of flavonoid, alkaloid, tannin, saponin, triterpenoid and steroid groups. Therefore, this study needs to be conducted to determine the secondary metabolite compounds contained in the Niko plant (Grewia koordersiana Burret) and the characterization of secondary metabolite compounds of Niko plant leaf extract (G. koordersiana Burret). Sample preparation, maceration, chromatography fractionation and characterization with UV-vis spectrophotometer and infrared spectroscopy. The dried leaves were ground by blending and weighed 200 g of G. koordersiana Burret leaf powder sample and macerated with 1 L of methanol solvent for 48 hours, then filtered. Furthermore, the extract was evaporated by vacuum rotary evaporator at 45oC and waterbath. Furthermore, phytochemical tests were carried out on the extract and fractionation using column chromatography and characterization. The results of the phytochemical test showed that the extract contained flavonoids, steroids and saponins. The results of the fractionation of methanol extract of niko leaves with column chromatography with methanol-ethyl acetate-chloroform eluent (1:2:3) obtained 25 fractions. The fractions obtained were subjected to KTL with a ratio of methanol-ethyl acetate-chloroform eluent (0.5:5:3) and 20 fractions were obtained. Furthermore, 20 fractions were subjected to TLC with a ratio of methanol-chloroform eluent (0.5:3) and the fractions with Rf 0.3 were fractions 2, 7, 9, 16, 17, and 18. Fractions with the same Rf were combined and concentrated to obtain a thick extract. The results of the UV-Vis spectrum showed that there was a π→π* transition which was conjugated C=C, and the n-π* electron transition was the chromophore group C=O. The results of the FTIR spectrum show that the wave numbers 3360 cm-1 and 3334.92 cm-1 are the (-OH) group and the wave number 2945.3 cm-1 is the C-H group and the wave number 2833.43 cm-1 is the aliphatic C-H group and the wave number 1600 cm-1 is the aromatic C=C group and the wave number 1026.13 cm-1 is the alcohol C-O group.
The Effect of Solvent Types on The Antibacterial and Cytotoxic Activity of Lino Bark Extract Landi Pote, Lodowik; Taek, Maximus M.; Nadut, Anggelinus; Latumakulita, Gertreda
Sains Natural: Journal of Biology and Chemistry Vol. 15 No. 3 (2025): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v15i3.825

Abstract

The G. koordersiana Burret plant, especially its bark, is used by the Sumba community to treat wounds, ulcers, and liver. This study aims to determine the effect of solvent types on the antibacterial and cytotoxic activity of G. koordersiana Burret extract. The research methods used were maceration method, antibacterial test, and cytotoxic test of G. koordersiana Burret bark extract. The results showed that the yield of ethanol, methanol, and n-hexane extracts were 42.15%; 41.65%; and 0.62%, respectively. Ethanol and methanol extracts had antibacterial activity against E. coli and S. aureus bacteria, while n-hexane extract did not show antibacterial activity against both bacteria. Cytotoxic tests showed that the LC50 values ​​of ethanol, methanol, and n-hexane extracts were 61.35 ppm; 758.58 ppm; and 2,494,181.19 ppm, respectively. Ethanol and methanol extracts had antibacterial activity and were toxic, while n-hexane extract did not show antibacterial activity and was not toxic. The antibacterial and cytotoxic activities of ethanol and methanol extracts showed the presence of secondary metabolite compounds of the alkaloid, phenolic, flavonoid, tannin, triterpenoid, and saponin groups, while n-hexane extract contained triterpenoid compounds that had no inhibitory power against bacteria or cytotoxic activity.
Elimination Of Manganese Interference With EDTA Masking In Determination of Nickel and Lead In Pyrolusite Using Atomic Absorption Spectrophotometry Landi Pote, Lodowik; Anggelinus Nadut
INDONESIAN JOURNAL OF CHEMICAL RESEARCH Vol. 8 No. 2 (2023): Volume 8, ISSUE 2,2023
Publisher : Chemistry Department, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijcr.vol8.iss2.art4

Abstract

ABSTRACT Research has been carried out on the elimination of manganese interference with EDTA masking in the determination of nickel and lead in pyrolusite using Atomic Absorption Spectrophotometry (AAS) with an air-acetylene flame. Dissolution of pyrolusite samples was carried out by wet digestion using aqueous regia and HF solutions with a ratio of 3:4 (v/v). Interference studies were carried out on the absorbance of nickel and lead with the addition of manganese with varying concentrations of 100-5000 μg/mL measured at a wavelength of 232.0 nm and a gap width of 0.2 nm for nickel and a wavelength of 283.3 nm and a gap width of 0.7 nm for lead. The analysis results show that manganese at a concentration of 100-5000 μg/mL can interfere with the absorbance of nickel and lead, where the absorbance of nickel and lead increases. Recovery results (Recovery) of nickel, lead and manganese with masking 0.10 M EDTA and extracting nickel, lead and manganese into chloroform with sodium diethyl dithiocarbamate complexing at pH 2 obtained nickel (85.39%), lead (92.63%) and manganese (0.00%). nickel and lead pyrolusite samples before extraction with an average nickel and lead content of 1817.82 ± 9.42 μg/g and 122.81 ± 7.60 μg/g. The nickel and lead content after manganese interference was removed by EDTA 0 masking .1 M at pH 2 and complexed with DDC in chloroform obtained an average content of 1571.32 ± 7.54 μg/g for nickel and 87.65 ± 7.53 μg for lead. Key words: Atomic Absorption Spectrophotometry, Interference, masking EDTA, Extraction, Nickel and Lead.
Co-Authors Anggelinus Nadut Anggelinus Nadut Dewi Q. M. Bulin, Christiani Elisabeth Ipih, Hapat Gerardus Diri Tukan Gertreda Latumakulita Klotilde Manek, Marliana Lalong, Paulus Risan F. Markus Taek, Maximus Maximus M., Taek Taek, Maximus M.