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All Journal Menara Perkebunan Bioscientist : Jurnal Ilmiah Biologi
Mumpuni, Siwi Putri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Induksi Kalus Krisan (Chrysanthemum morifolium var. reagen pink) dengan Penambahan Naphtalen Acetic Acid (NAA) dan Kinetin Secara In-Vitro Sholehah, Washeilatus; Resmisari, Ruri Siti; Oktafia, Safina; Mumpuni, Siwi Putri
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13217

Abstract

Chrysanthemum morifolium var. reagent pink is an ornamental flower that is widely cultivated in Indonesia. The increasing demand makes it difficult for farmers to fulfill the market. In-vitro tissue culture is an effective solution to multiply chrysanthemum seedlings in bulk and quickly. This study aims to identify the most effective concentration in inducing callus in chrysanthemum plants by combining Naphtalen Acetic Acid (NAA) and kinetin. This study was an experimental research with a completely randomized design (CRD), involving 12 treatments and each treatment was repeated 4 times. There were two treatment factors; NAA concentration (0 mg/l, 0.5 mg/l, 1 mg/l) and kinetin concentration (0 mg/l, 1 mg/l, 2 mg/l, 3 mg/l). Parameters in this study included days to callus appearance, percentage of callus explants, callus wet weight, callus color, and callus texture. Data were analyzed using analysis of variance (ANOVA). The results showed that (1) the fastest callus was formed at 11 HST by giving the treatment of 0 mg/l NAA + 3 mg/l kinetin; (2) the optimal treatment for the percentage of callus explants was obtained by giving 1 mg/l NAA + 1 mg/l kinetin); (3) the optimal treatment of callus wet weight was obtained by giving 1 mg/l NAA + 2 mg/l kinetin; (4) the best callus quality was obtained by giving a combination of NAA 1 mg/l and kinetin 1 mg/l, with a crumbly texture and brownish yellow callus.
In silico test of brotowali (Tinospora crispa) as potential anticancer agent targeting mTOR on colorectal cancer Isnaini, Hanik; Agustin RJ, Adinda Amalia; Sholehah, Washeilatus; Sudarlina, Nida; Oktafia, Safina; Khodariyah, Luluk Ayu; Mumpuni, Siwi Putri
Menara Perkebunan Vol. 93 No. 1 (2025): 93(1), 2025
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v93i1.595

Abstract

Colorectal cancer is one of the deadliest diseases in the world. Treatment to overcome colorectal cancer has been pursued in such a way. Still, theresults are unsatisfactory, so treatment turns to herbal plants such as brotowali (Tinospora crispa) as an alternative colorectal anticancer. T.crispa  is one of the herbal plants that contains a typical compound in the form of N-acetylnornuciferine and alkaloid-derived active compounds in the form ofN-formylanonaine, N- trans-coumaroyltyramine, and Tyramine which are potential as colorectal anticancer agents. The study used the mTOR(Mammalian Target of Rapamycin) receptor with PDB code (4DRJ) with its native ligand, RAP (receptor-associated protein). This study aims to analyze the potential of colorectal anticancer targeted by active compounds of alkaloid derivatives in T.crispa against mTOR. The method used is to explore active compounds through the KnapSack web and 3D protein structures on NCBI, docking validation using PyMol, tethering compounds N-acetylnornuciferine, N-formylanonaine, N-trans-coumaroyltyramine, and Tyramine to the target ligand using Pyrx and then visualized using BIOVIA Discovery Studio Visualizer. Analysis of the in silico test results showed that the compounds of N-acetylnornuciferine, N-trans-coumaroyltyramine, and Tyramine in T.crispa have good potential as colorectal anticancer agents in low doses but are not recommended for people with heart disease.
Induksi Kalus Krisan (Chrysanthemum morifolium var. reagen pink) dengan Penambahan Naphtalen Acetic Acid (NAA) dan Kinetin Secara In-Vitro Sholehah, Washeilatus; Resmisari, Ruri Siti; Oktafia, Safina; Mumpuni, Siwi Putri
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13217

Abstract

Chrysanthemum morifolium var. reagent pink is an ornamental flower that is widely cultivated in Indonesia. The increasing demand makes it difficult for farmers to fulfill the market. In-vitro tissue culture is an effective solution to multiply chrysanthemum seedlings in bulk and quickly. This study aims to identify the most effective concentration in inducing callus in chrysanthemum plants by combining Naphtalen Acetic Acid (NAA) and kinetin. This study was an experimental research with a completely randomized design (CRD), involving 12 treatments and each treatment was repeated 4 times. There were two treatment factors; NAA concentration (0 mg/l, 0.5 mg/l, 1 mg/l) and kinetin concentration (0 mg/l, 1 mg/l, 2 mg/l, 3 mg/l). Parameters in this study included days to callus appearance, percentage of callus explants, callus wet weight, callus color, and callus texture. Data were analyzed using analysis of variance (ANOVA). The results showed that (1) the fastest callus was formed at 11 HST by giving the treatment of 0 mg/l NAA + 3 mg/l kinetin; (2) the optimal treatment for the percentage of callus explants was obtained by giving 1 mg/l NAA + 1 mg/l kinetin); (3) the optimal treatment of callus wet weight was obtained by giving 1 mg/l NAA + 2 mg/l kinetin; (4) the best callus quality was obtained by giving a combination of NAA 1 mg/l and kinetin 1 mg/l, with a crumbly texture and brownish yellow callus.
Co-Authors Agustin RJ, Adinda Amalia Isnaini, Hanik Khodariyah, Luluk Ayu Oktafia, Safina Ruri Siti Resmisari Sholehah, Washeilatus Sudarlina, Nida