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In Vitro Growth Response of Porang (Amorphophallus muelleri Blume) Bulbil to Kinetin Concentration Normasari, Rut
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.9050

Abstract

The plant known as porang (Amorphophallus muelleri Blume) has a high glucomannan content in its tubers. The availability of porang seeds is one of the elements influencing porang production, hence tissue culture must be developed to improve seed procurement. For porang seed propagation to be successful in vitro, growth regulators must be added. This study aims to determine the effect of adding kinetin concentration on porang shoot induction. Five treatments the addition of kinetin at doses of 0, 1, 2, 3, and 4 mg/L were included in the Non-Factorial Completely Randomized Design (CRD) experiment. All observation variables, including explant growth, shoot emergence time, percentage of shoots created, total number of shoots, and number of shoots per explant, were found to be significantly impacted by the addition of kinetin. With a shoot emergence time of 21.86 days after inoculation, 96% of the shoots produced, a total number of 108.80 shoots, and a number of shoots of 22.75 per explant, kinetin 4 mg/L was the optimal concentration for porang shoot induction.
Influence of Photoperiod on The Induction of in Vitro Porang (Amorphophallus muelleri Blume) Shoots Normasari, Rut
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.7965

Abstract

The tuber plant known as porang (Amorphophallus muelleri Blume) has a lot of industrial potential. Due to the restricted supply of seeds, porang production is still minimal despite its many advantages, which make it an export commodity with plenty of prospects. An alternate method for quickly and efficiently producing high-quality porang seeds in big quantities is in vitro culture. One significant element influencing in vitro shoot induction is photoperiod. The study aims to obtain the best photoperiod for in vitro porang shoot induction. This study applied a completely randomized design with 7 photoperiod treatments (0, 4, 8, 12, 16, 20 and 24 hours) and 5 replications. The percentage of explants that develop shoots, the number of shoots per explant, and the total number of shoots are all examples of observation parameters. An additional 5% Duncan test was performed to determine whether there was a significant impact on the observation variables after the observation data was analyzed using analysis of variance. The results showed that a 16-hour photoperiod produced the highest porang shoot induction in all observation variables in the 4th and 8th weeks.