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All Journal Jurnal Biologi Tropis Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Bioscientist : Jurnal Ilmiah Biologi
Hany, Iga Permata
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Effect of Kinetin Concentration on Callus Induction of Cryptocarya massoy (Oken) Kosterm Under in Vitro Conditions Noli, Zozy Aneloi; Hanafi, Muhammad; idris, M.; Hany, Iga Permata
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8162

Abstract

The Papuan indigenous plant Cryptocarya massoy (Oken) Kosterm has substantial commercial significance as an essential oil source. Plant growth regulators (PGRs) are essential for regulating growth responses including callus development, and tissue culture offers a viable means of reproducing this species. Kinetin, a cytokinin-type PGR, is particularly important in promoting cell division and callus development. This study aimed to evaluate the growth response of C. massoy explants under varying kinetin concentrations and determine the optimal concentration for callus induction. The experiment utilized a Completely Randomized Design (CRD) with three kinetin treatments: 0.5 mg/L (A), 1.0 mg/L (B), and 1.5 mg/L (C). Results showed that 1.0 mg/L kinetin achieved the highest explant survival rate (71%) and the lowest browning rate (8%), compared to 13% browning at 0.5 mg/L and 1.5 mg/L. However, the highest callus formation (0.25 average callus) with a compact, brownish texture was observed at 1.5 mg/L. The 0.5 mg/L concentration consistently exhibited the lowest response across all parameters. These findings indicate that 1.0 mg/L kinetin is optimal for enhancing explant survival and minimizing browning, while 1.5 mg/L is more effective for inducing callus formation, underscoring the importance of kinetin concentration in optimizing C. massoy tissue culture protocols.
Induksi Tunas Anggrek Dendrobium discolor dengan Penambahan Konsentrasi 2,4-D secara In vitro Hany, Iga Permata; Noli, Zozy Aneloi; Idris, M.
AGRITROP Vol 21, No 2 (2023): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v21i2.21095

Abstract

Dendrobium discolor merupakan salah satu anggrek yang memiliki keunikan pada bentuk dan warna bunganya. Bunga yang melengkung ke arah dalam dengan warna kuning keemasan membuat anggrek ini semakin diminati. Kemampuan regenerasi anggrek secara umum sangat lambat jika dibiarkan pada kondisi alamiahnya. Propagasi melalui induksi tunas secara in vitro merupakan usaha perbanyakan yang paling tepat untuk memperbanyak anggrek ini. Penggunaan ZPT 2,4-D mampu menginduksi tunas dengan berperan pada pembelahan sel eksplan D. discolor. Penelitian ini bertujuan untuk melihat pengaruh beberapa konsentrasi ZPT 2,4-D dan menemukan konsentrasi terbaik dalam menginduksi tunas anggrek D. discolor secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan beberapa konsentrasi 2,4-D: A (1 mg/L), B (2 mg/L), C (3 mg/L), dan D (4 mg/L). Hasil penelitian ini menunjukkan bahwa semua perlakuan penambahan 2,4-D dapat memberikan persentase eksplan hidup sebesar 100%. Waktu muncul tunas tercepat ditunjukkan oleh perlakuan 4 mg/L. Propagasi melalui induksi tunas secara in vitro dengan penambahan ZPT 2,4-D sebanyak 4 mg/L merupakan protokol perbanyakan yang optimum untuk anggrek D. discolor.
Micropopagation of Cryptocarya massoy (Oken) Kosterm. Trough Optimizing Callus Induction with Benzylaminopurine (BAP) Supplementation Hanafi, Muhammad; Noli, Zozy Aneloi; Idris, M.; Hany, Iga Permata
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.13852

Abstract

This study aimed to evaluate the effect of BAP concentrations on explant response and callus formation in Massoia. The experiment was arranged in a Completely Randomized Design (CRD) with three BAP concentrations: 0.5 mg/L, 1.0 mg/L, and 1.5 mg/L, each with 24 replications. The culture medium used was Murashige and Skoog (MS), and observations were carried out for 7 days after planting. Parameters observed included the percentage of viable explants, browning, contamination, average callus formation, callus color, and texture. Data were analyzed using analysis of variance (ANOVA) at a 5% significance level. The results showed that 0.5 mg/L BAP yielded the highest explant survival rate (54%), the lowest browning rate (13%), and 35% contamination. Meanwhile, 1.5 mg/L BAP produced the highest average callus formation (0.21) with compact texture and brown coloration. Although not statistically significant, BAP concentrations affected explant viability and callus formation. A concentration of 0.5 mg/L tended to be optimal for maintaining explant viability, while 1.5 mg/L was more effective in inducing callus. These findings provide a preliminary basis for optimizing Massoia tissue culture techniques.
Co-Authors M. Idris Muhammad Hanafi Zozy Aneloi Noli