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All Journal Journal of Multidisciplinary Applied Natural Science Electronic Journal of Education, Social Economics and Technology
Rini Purbowati
Biomedical Department And Biomolecular Research, Universitas Wijaya Kusuma Surabaya, Surabaya-60225 (Indonesia)
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Detection of csg and lux Genes in Biofilm-Forming Uropathogenic Escherichia coli Associated with Urinary Tract Infections Rini Purbowati; Sri Lestari Utami; Dadik Raharjo; Masfufatun Masfufatun
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.222

Abstract

Uropathogenic Escherichia coli (UPEC) is responsible for 80–90% of urinary tract infections (UTI) in the global population. The emergence of the increasing resistance to broad-spectrum antimicrobial agents was due to the ability to form biofilms. Cell surface factors that play a role in biofilm formation include Quorum Sensing (QS) which is encoded by the luxS family gene and curli by two operons, namely the csgBA operon. The purpose of the study is to detect the effects of 2 virulence genes (csgD and luxS) on biofilm-forming UPEC associated with UTI. As many as 76 UPEC isolates were collected from the clinical microbiology laboratories and the biofilm development was analyzed using the crystal violet method on microplate 96 wells. Using PCR assay, the two studied genes (csgD and luxS) were determined to be present in the isolates. UPEC isolates the bacteria-produced biofilms (90.80%) and nonproducers (9.20%). Most UPEC bacteria (97.36%) are known to be positive for csgD and luxS gene, while the others (92.10%) are known to be positive for the luxS gene. The highest proportion of the genes expressed in this study is followed by the presence of a relationship between the ability to produce biofilm and the presence of the genes under investigation, which is followed by all UPEC strains that cause UTI in humans.
The Overview of the Effectiveness of Infrared on the Healing Rate of Incision and Burn Wounds in Male White Rats Fuad Ama; Rini Purbowati; Emillia Devi Dwi Rianti
Electronic Journal of Education, Social Economics and Technology Vol 5, No 2 (2024)
Publisher : SAINTIS Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33122/ejeset.v5i2.235

Abstract

Infrared rays have low energy and their use does not cause toxic effects on the environment, so that infrared rays can balance and activate cells in the body, thin the blood, break down water molecules, and inhibit bacteria or fungi. Inhibition of bacterial or fungal growth in skin wounds that are often experienced and cause damage to epithelial tissue. The purpose of the study provides an overview of the effectiveness of infrared on the healing rate of incision wounds and burns in Wistar rats. The method with an experiment with a completely randomized design method, the sample used was 48 male white rats with 24 with incision wounds and 24 with burns. The results of the incision wound on the 12th day showed that there was a reduction in the length of the wound at P1 (0.1 cm), P2 (0.1 cm) and P3 (0.2 cm) so that with a wound length of 0.1 means healing has occurred. Burns on the 12th day there was a decrease in the diameter of the burn wound both at P1 = P2 = P3 = 0.3 cm. Conclusion The use of infrared therapy in the healing process of incision wounds and burns on days 9-12. Infrared wavelength 940 nm as near infrared produces heat, so the healing process is less than 14 days.
Co-Authors Dadik Raharjo Emillia Devi Dwi Rianti, Emillia Devi Dwi Fuad Ama Masfufatun Masfufatun Sri Lestari Utami