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All Journal El-Hayah : Jurnal Biologi Jurnal Berkala Epidemiologi Indonesian Journal of Tropical and Infectious Disease TEKNOLOGI PANGAN : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Nusantara Science and Technology Proceedings Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Multidisciplinary Applied Natural Science AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Jurnal Kedokteran Hewan Jurnal Agro Veteriner Folia Medica Indonesiana
Dadik Raharjo
Department Of Veterinary Public Health, Faculty Of Veterinary Medicine, Universitas Airlangga
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THE CLINICAL EPIDEMIOLOGY OF NOROVIRUS INFECTION IN CHILDREN WITH DIARRHEA AT REGIONAL PUBLIC HOSPITAL DR.SOETOMO Alpha Fardah Athiyyah; Sofia Wardhani; Andy Darma; Reza Gunadi Ranuh; Dadik Raharjo; Toshiro Shirakawa; Subijanto Marto Sudarmo
Jurnal Berkala Epidemiologi Vol. 8 No. 3 (2020): Jurnal Berkala Epidemiologi (Periodic Epidemiology Journal)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbe.V8I32020.200-207

Abstract

Background: Norovirus has become a major cause of severe outbreaks of gastroenteritis since the discovery of the rotavirus vaccine, with the main symptom being diarrhea. Until now, research on the epidemiological analysis of norovirus has not been carried out at Regional Public Hospital (RSUD) Dr. Soetomo, Surabaya. Purpose: This study aims to provide clinical epidemiology data and an analysis of norovirus infections in children with diarrhea at Regional Public Hospital (RSUD) Dr. Soetomo, Surabaya, Indonesia, including the prevalence of norovirus infection in each age group and sex, its clinical appearance, and its seasonal variation. Methods: A cross-sectional study was conducted in children aged 1–60 months hospitalized for diarrhea in RSUD Dr. Soetomo between April 2013 and March 2014. Identification of the virus in the stool was done by norovirus enzyme immunoassay Quick NaviTM Noro2. The proportion, age, sex, clinical symptoms, and patterns of the norovirus seasonal data were calculated. Results: Norovirus was detected in 64 samples (19%) of the 340 stool samples, with a mean patient age of 11.75 months; it was mostly found in patients less than 24 months of age (95%), and 64% were male. The monthly pattern of norovirus infection was mostly found in November, followed by May and April. The clinical symptoms were fever (72%), vomiting (66%), bloating (59%), abdominal cramps (34%), perianal inflammation (27%), abdominal distension (16%), and seizures (8%). Conclusion: The prevalence of norovirus was found to be high in patients with diarrhea aged 1–60 months hospitalized in RSUD Dr. Soetomo, with a proportion of 19%. Further research is needed to determine the severity of norovirus infection.
Occurrences Salmonella sp. and Escherichia Coli in Bulk and Packaged Chicken Sausages in Surabaya, Indonesia Deana Fyra Adi Nur; Ratna Yulistiani; Dedin F. Rosida; Dadik Raharjo
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 6 No. 2 (2022)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.656 KB) | DOI: 10.29165/ajarcde.v6i2.99

Abstract

Salmonella sp. and Escherichia coli are pathogenic bacteria that cause foodborne diseases that often contaminate food and are harmful to human health. Sales of unpackaged (bulk) sausages accompanied by poor hygiene and environmental sanitation conditions have a high potential for Salmonella and Escherichia coli contamination. The purpose of this study was to compare the contamination level of bacterial, Salmonella sp. and Escherichia coli in bulk and packaged chicken sausages as well as to determine the relationship between the hygiene and sanitation of traders and the level of bacterial contamination, Salmonella sp. and Escherichia coli contamination in chicken sausages sold at traditional markets in Surabaya, Indonesia. This research is a cross-sectional study and purposive sampling method. The results showed that the level of bacterial contamination in the bulk chicken sausage was higher (5.98 Log CFU/g) than in packaged chicken sausage (4.83 Log CFU/g). Salmonella sp. contamination in the bulk chicken sausage was higher (44.44%) than in packaged chicken sausage (10.00%) and Escherichia coli contamination in the bulk chicken sausage was higher (22.22%) than in packaged chicken sausage (20.00%). There is a significant relationship between the hygiene and sanitation of traders with the contamination level of bacteria, Salmonella sp., and Escherichia coli in bulk and packaged chicken sausages.
Bacterial Contamination on Meatballs Sold in Traditional Markets P.D. Surya Market in East Surabaya Region Causes a Decline in People's Income Alfiyatus Sholichah; Ratna Yulistiani; Ulya Sarofa; Dadik Raharjo
Budapest International Research and Critics Institute-Journal (BIRCI-Journal) Vol 5, No 4 (2022): Budapest International Research and Critics Institute November
Publisher : Budapest International Research and Critics University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33258/birci.v5i4.7290

Abstract

Coliform, Escherichia coli and Salmonella sp. are bacterial pathogen that causes foodborne disease which often contaminates food that is harmful to human health. The sale of meatballs without packaging accompanied by poor hygiene and environmental sanitation conditions has a high potential for contamination by Coliform, Escherichia coli and Salmonella sp. The purpose of this study was to identify the contamination of Coliform, Escherichia coli and Salmonella sp in bulk and packaged meatballs sold at PD Pasar Surya in the East Surabaya Region and to determine the relationship between the hygiene and sanitation of traders on the level of bacterial contamination, Coliform contamination, Escherichia coli and Salmonella sp in bulk and packaged meatballs. This research is a cross sectional study and the sample was taken by purposive sampling method. The relationship between hygiene and sanitation of traders with microbiological quality of bulk meatballs and packaged meatballs using Chi Square. The results showed that the level of total bacterial contamination was higher in bulk meatballs (66.6%) compared to packaged meatballs (40%), Total Coliform bulk meatballs were 60% higher than packaged meatballs (40%), Escherichia coli meatballs Bulk meat (40%) is higher than packaged meatballs (20%), Salmonella sp in bulk meatballs (33.3%) is higher than packaged meatballs (40%). There is a significant relationship between the hygiene and sanitation of traders and the level of bacterial contamination, Coliform, Escherichia coli and Salmonella sp.
Tingkat cemaran bakteri Coliform dan Escherichia coli pada makanan dan minuman sebagai dampak kondisi higiene sanitasi di Sentra Kuliner Penjaringansari, Surabaya Ratna Yulistiani; Jariyah; Dadik Raharjo; Ulya Sarofa; Dinda Alvianita Sabrina

Publisher : Program Studi Ilmu dan Teknologi Pangan, Universitas Yudharta, Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/tp.v14i1.3565

Abstract

???????????? ???????????????????????????????? ???????? ???????????????????????????????? ???????????????????????????????? ???????????? ???????????????????????????????????????????? ???????????????? ???????? ???????????????? ???????? ???????????????????????????????????? ???????? ???????? ???????????????????????????????????? ???????? ???????????????? ???????????????????????????? ???????????? ???????????????????????????????????????? ???????????? ???????????????? ???????????????????????????? ????????????????????????????????????. ???????????????? ???????????????????????????????? ???????? ???????? ???????????????????????????????????????????????????? ????????????????????, ???????????????????????????? ???????????????? ???????????????? ???????????????? ???????????? ???????????????????????????????????? ???????????????? ???????? ???????????????????????????? ???????? ???????????? ???????????????????????????????????????????? ???????????????? ???????????????????????????????? ???????????????????????? ???????? ????????????????????????????????. ???????????? ???????????????? ???????? ???????????????? ???????????????????? ???????????? 1). ???????????????????????????????? ???????????? ???????????????????????????? ???????????? ???????????????????????????????????????? ???????????????????????????????????????? ???????? ???????????????? ???????????????????????????? ????????????????????????????????????; 2). ???????????????????????????????????? ???????????? ???????????????? ???????????????????????? ???????? ???????????????? ???????????? ???????????????????????????????? ???????????????????????????????? ???????? ???????????????????? ???????? ???????????? ???????????????????????????????????? ???????????????????????????????????????????????????? ????????????????????, ???????????????????????????????? ???????????? ???????????????????????????????????????????? ???????????????? ????????????????????????????????????????????????????; 3). ???????????????????????????? ???????????? ???????????????????????????????????????????????? ???????????????????????????? ???????????? ???????????????????????????? ???????????? ???????????????????????????????????????? ???????? ???????????????????????????? ???????? ???????????????????????????????? ???????????? ???????????????????????????????????????????? ???????????????? ????????????????????????????????????????????????????; 4). ???????????????????????????????? ???????????????????????????? ???????? ???????????????????????????????????? ????????????????????????????????????????????????????. ???????????? ???????????????????????????? ???????????? 41.67% ???????? ???????????????? ???????????????????????????? ???????????????????????????????????????? ???????????????????? ???????????????????????????????? (???????????????????? 2.7 ???? 104 -3.8 ???? 105 ????????????????????????????????/????????????????) ???????????????????????????????????? ???????????? ???????????????????????????? ???????????????????? ???????? ????????????????. ???????????????????????????????? ???????????????????????????? ???????????????????????? ???????????????? 100% ???????? ???????????????? ???????????????????????????? ???????????? 66.6% ???????? ???????????????????????? ???????????? ???????????????????????? ???????????????????????????? ???????????? ???????????????????????????????? ???????????? ???????????????????????????? ???????????????????? ???????? ???????????????? ???????????????????????????????????????????????? (???????????????? ???????????? ???????????????????? ???????????????????????????? >3 ????????????/????; ???????????????????????? > 0 ????????????/????) ???????????? 50% ???????? ???????????? ???????????????????????????? ???????????????????????????????????? ???????????????????????????????????????????? ???????????????? ???????????????????????? ???????????????? ????????????????????????????????????. ???????????????????? ???????? ???? ???????????????????????????????????????????? ???????????????????????????????????????????????? (????<0.05) ???????????????????????????? ???????????? ???????????????????????????????????????? ???????????????????????????? ???????? ???????????????????????????? ???????????????? ???????????????????? ???????????????????????????????? ???????????? ???????????????????????????????????????????? ???????????????? ???????????????????????????????????????????????????? ???????? ????????????????, ???????????????????????????????????? ???????????? ????????????????????????. ???????????????????????????? ????????????????????????????????, ????????????????????????, ???????????? ???????????????????? ???????????? ???????????? ???????????????????? ???????? ???????????????????????????????? ???????????? ???????????????????????????? ???????? ???????????????????????????????? ????????????????????????????????????????????????????.
Logo, Label, and Packaging Improvement on Jamu Bu Uri Products for Digital Marketing Kartini; Ratna Yulistiani; Dadik Raharjo; Rizky Fatkhur Rohman; Sri Alam Syah Lihan Candra Asi
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3342

Abstract

It is quite common knowledge that some traditional herbal medicine sellers used plastic bottles of mineral water as a storage medium for their traditional herbal medicine. Even though the allotment of mineral water bottles is dispossible, it cannot be used again after the contents run out. This problem about the packaging must be addressed. When viewed from the visual aspect, the presentation of traditional herbal medicine still tends to be monotonous in terms of packaging. Whereas packaging can influence consumers to give a positive response, good packaging will optimize profits. Therefore, this service activity targets several improvements in handling packaging problems for Jamu bu Uri products, with the aim of increasing the marketing of traditional herbal products.
Prevalence and Infestation of Ectoparasite in Dabung Ducks in Some Districts in Bangkalan Madura Prawasa, Ramadhiniyanti Putri Alif; Nunuk Dyah Retno Lastuti; Dadik Raharjo; Poedji Hastutiek; Endang Suprihati; Dian Ayu Permatasari
Journal of Parasite Science Vol. 7 No. 2 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i2.40134

Abstract

Identify the type of ectoparasite prevalence and infestation pattern of ectoparasite on Dabung duck in Bangkalan Madura regency. This research conducted from February to April 2022 with sample of 55 tails and identified in parasitology laboratory of the Veterinary Parasitology Division, Faculty of Veterinary Medicine, Airlangga University, Surabaya with the permanent mounting method without coloring. This research was a descriptive research, study survey, and cross sectional study analytical. The type of ectoparasite that attacked Dabung duck in Geger subdistrict, Dabung village and Galis subdistrict, Pekaan Dajah village were Menacanthus stramineus, Menopon gallinae, Lipeurus caponis with a prevalence of 100% from a sample 55 tails of Dabung duck. The analysis results of infestation pattern of ectoparasite infestation on Dabung ducks used correspondence text on the body regions of Dabung ducks in Geger subdistrict, Dabung village showed that M. stramineus ticks on chest region, M. gallinae in head neck region and L. caponis in wing region. Burneh subdistrict, Burneh village founded M. stramineus ticks in abdomen region, M. gallinae in head-neck region, and L. caponis in wing region. Galis subdistrict, Pakaan Dajah village showed that M stramineus lice in head-neck region, L. caponis in wing region and M. gallinae in spine region.
Antimicrobial Activities of Laurus nobilis Leaves Ethanol Extract on Staphylococcus aureus, Salmonellae typhi, and Escherichia coli. Sakran, khawla Abdullah; Raharjo, Dadik; Mertaniasih, Ni Made
Indonesian Journal of Tropical and Infectious Disease Vol. 9 No. 2 (2021)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v9i2.22873

Abstract

Laurus nobilis is one of the most well-known, most frequently used plants is from Lauraceae family which contains up 2.500 species that grow in the subtropics and tropics of the Mediterranean region and Indonesia. This study was supposed to investigate the antimicrobial eff ect of L.nobilis leaves ethanol extract on Staphylococcus aureus, Salmonellae typhi, and Escherichia coli. This preliminary study examined the antimicrobial eff ect of L.nobilis leaves ethanol extract. The method used Agar-well diff usion for determination of the zone of inhibition and the minimum bactericidal concentration to investigate the activity of L.nobilis leaves ethanol extract at 100% concentration . The results revealed that extract of L. nobilis leaves had the antibacterial activity against Staphylococcus aureus with a zone of inhibition (16.3 ±1.5 mm), Staphylococcus aureus with (14.5±0.5 mm), and weak antimicrobial activity against Escherichia coli (11.3±1.1mm). Also, through the minimum bactericidal concentration experiment, the L.nobilis leaves ethanol extract had activity on Staphylococcus aureus and Salmonellae typhi, it's killed the bacteria in all concentration start it from 5í—107 to 5í—104. But the activity on Escherichia coli just weaken concentration 5í—107 and 106. This research has concluded that the L.nobilis leaves ethanol extract exhibited a signifi cant antimicrobial eff ect against Staphylococcus aureus and Salmonellae typhi then Escherichia coli that is considered a kind of multidrug-resistant bacteria.
Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya Mutiarasari, Nonie Olivia Adia; Harijani, Nenny; Rantam, Fedik Abdul; Raharjo, Dadik; Estoepangestie, Agnes Theresia Soelih; Handijatno, Didik
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28584

Abstract

This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identification. The identification of S. aureus consists of isolation in Mannitol Salt Agar, Gram staining, catalase test, and coagulase test. Total plate count result showed that four samples were exceeding the National Standard of Indonesia SNI 7388:2009 or 1x106 CFU/g and the rest were below the maximum Total Plate Count in SNI. The highest Total Plate Count result was 1,9x106 CFU/g and the lowest was 7,8x104 CFU/g. The result of identification showed that 100% samples examined were contaminated by S. aureus with the highest result was 2,9x104 CFU/g and the lowest result was 4,3x103 CFU/g or exceeding the SNI 7388:2009.
Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya Amrullah, Amirul Muslim; Rantam, Fedik Abdul; Raharjo, Dadik; Tyasningsih, Wiwiek; Estoepangestie, Agnes Theresia Soelih; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.31134

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine's Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).
The Quality of Milk Production in Friesian Holstein (FH) Dairy Cattle Experiencing Repeat Breeding at KUD Tani Wilis Sendang, Tulungagung Regency Sintya Kumalasari Wibowo; Aldin Akbar Rahmatullah; Cindy Ercha Aulia Putri; Pudji Srianto; Dian Ayu Permatasari; Dadik Raharjo; Rimayanti; Erma Safitri; Mohammad Auzaie Afandi; Nuurin Ajrin Karim
Journal of Applied Veterinary Science And Technology Vol. 5 No. 2 (2024): October 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I2.2024.135-139

Abstract

Background: Repeat breeding is a notable reproductive problem where cows do not conceive after several insemination attempts, and it is typically defined as three or more unsuccessful attempts. This results in reduced reproductive efficiency, lower economic sustainability of the dairy farm, and decreased milk quality in dairy cattle.  Purpose: The purpose of this study was to investigate changes in milk quality in Friesian Holstein (FH) dairy cows subjected to repeat breeding at KUD Tani Wilis Sendang, Tulungagung Regency, focusing on the milk’s density, as well as fat, Total Solid (TS), and Solid Non-Fat (SNF) content. Method:  Thirty-two milk samples were collected, including 10 from normal cows for comparison. The samples were analyzed using a Lactoscan to examine the density, fat content, total solid (TS), and solid non-fat (SNF) of milk. The data was subjected to Independent T-Test analysis. Results: The results revealed significant differences (p<0.05) between repeat breeding and normal cow milk samples in terms of specific gravity, fat content, and Total Solids (TS), but not in Solid Non-Fat (SNF). Repeat breeding cow milk exhibited a higher specific gravity (1.0282 vs. 1.0260) but lower fat content (1.08% vs. 4.18%) and Total Solids (9.38% vs. 12.73%) compared to normal cow milk. However, there was no significant difference for Solid Non-Fat (8.36% for repeat breeding vs. 8.55% for normal cows). Conclusion: Repeat breeding cow milk showed increased specific gravity but decreased fat content and Total Solids compared to normal cow milk, while Solid Non-Fat remained relatively consistent.
Co-Authors Adiana Mutamsari Witaningrum Agnes Theresia Soelih Estoepangestie Aldin Akbar Rahmatullah Alfiyatus Sholichah Alpha Fardah Athiyyah Amrullah, Amirul Muslim Amrullah, Muhammad Fajar Andy Darma Budiarto Budiarto Budiarto Bugiono, Bugiono Caska - Cindy Ercha Aulia Putri Deana Fyra Adi Nur Dedin F. Rosida Dian Ayu Permatasari Didik Handijatno Dinda Alvianita Sabrina Endang Suprihati Eriza Rosalina Rochmah ERMA SAFITRI Erma Safitri Erma Safitri Fedik Abdul Rantam Hasibuan, Sandi Aziz Hidanah, Sri I Gusti Made Reza Gunadi Ranuh Ikhsan, Dilla Amalia Bilqis Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Jariyah Kartini Khairullah, Aswin Rafif Lucia Tri Suwanti, Lucia Tri Masfufatun Masfufatun Mohammad Auzaie Afandi Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nenny Harijani Ni Made Mertaniasih Nunuk Dyah Retno Lastuti Nur, Deana Fyra Adi Nusdianto Triakoso Nuurin Ajrin Karim PA, Deanty Ayu Pasang, Gabriel Sampe Poedji Hastutiek Prawasa, Ramadhiniyanti Putri Alif Pudji Srianto Putri, Natalie Lovena Pramono Ratna Yulistiani Rimayanti Rimayanti Rimayanti Rimayanti Rimayanti Rini Purbowati Rizky Fatkhur Rohman Rosida, Dedin F. Sakran, khawla Abdullah Setyawan, Boedi Shirakawa, Toshiro Sintya Kumalasari Wibowo Sintya Kumalasari Wibowo Soeharsono Soeharsono Sofia Wardhani Sri Alam Syah Lihan Candra Asi Sri Hidanah Sri Lestari Utami Sudarmo, Subijanto Marto Supriyadi Supriyadi Tacharina, Martia Rani Tita Damayanti Lestari Toshiro Shirakawa Tyasningsih, Wiwiek Ulya Sarofa Ulya Sarofa Warsito, Sunaryo Hadi Widya Paramita Lokapirnasari