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Gandanegara, S
Research Center for Biology-Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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PENGARUH INOKULASI SEJUMLAH ISOLAT AZOSPIRILLUM TERHADAP PERTUMBUHAN DAN SERAPAN PUPUK N PADA TANAMAN JAGUNG Gandanegara, S; Slamet, S; Idawati, Idawati; Lina, M
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.229 KB) | DOI: 10.14203/beritabiologi.v6i3.1222

Abstract

Two greenhouse experiments had been conducted to study the effect of inoculation of some Azospirillum isolates on maize growth in sand culture and sterilized soil. In the first experiment some growth parameters were used, i.e., root length and root surface area, dry weight of root, shoot, and shoot length. Inoculation enhanced plant growth, as shown by increased of all plant growth parameters. Root length and root surface area were sensitive parameter and could be used for evaluating the effect of inoculation. The best plant perfomance achieved by inoculation with Azospirillum isolates Btl No. 8 and No. 11 and with the increase of plant biomass 24 and 53% over control, respectively. In the second experiment, six selected Azospirillum isolates were evaluated on growth, N uptake, and N contribution15 in maize plants. N isotope dilution method was used to measure the N contribution from fertilizer. Uninoculated control plants showed low dry weight and contribution from N fertilizer, 3.09 gram and 10.84%. Inoculation increased mineral nutrition expressed in higher N plant yield and N contribution from fertilizer. N plant yield ranging from 147 to 189 mg N as compared to 134 mg N in control plants. The high contribution of N fertilizer (13.21-14.35 %) achieved by inoculation with Azospirillum isolates No. 6, 7, 8, and RDCB Az 1
EFEKTIVITAS INOKULAN BRADYRHIZOBIUM DAN GALUR MUTAN KEDELAI TERHADAP KANDUNGAN N DAN HASIL DI LAHAN MASAM Gandanegara, S; Wemay, J; Idawati, Idawati; Ardjasa, Wayan Sabe
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.666 KB) | DOI: 10.14203/beritabiologi.v5i1.1101

Abstract

One field experiment had been conducted at Institute for Research and Agricultural Technology Evaluation at Taman Bogo FieldSta,to screen some Bradyhyzobium inoculants on soybean mutant lines developed for acid soil.Inoculants evaluated were single strain B-22,and its mixed inoculants (B-22+B37), (B-22 + G49), and (B-22+TAL 102) on two soybean mutant lines No.07 and 58,and cv.Wilis as check variety. Plant growth and N yield were determined at pod development (R4) and grain maturity (R8) stages.The effect of inoculation significantly influenced nodul-ation and N yield and tended to increase plant dry weight 25%.Mixed inoculant (B-22+B-37) showed good symbiotic compatibility with the three soybean genotypes tested,whereas inoculant(B- 22+G49) only showed good compatibility with mutant line No.58.Cultivar Wilis had broader symbiotic spectrum as compared to mutant line No. 07 and 58.The effect of plant genotypes,and its interaction with inoculants were significant on grain and N yield.It was observed that at pod development stage,the plant growth of mutant line No. 07 were slower than the other genotypes but it had better nutrient translocation rate to grain which resulted grain yield of cv. Wilis. The results showed that this mutant line could be developed for this area.
PERBANYAKAN TANAMAN KRISAN (Chrysanthemum morifolium) MELALUI KULTUR JARINGAN Dwimahyani, Ita; Gandanegara, S
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.124 KB) | DOI: 10.14203/beritabiologi.v5i4.1127

Abstract

Vegetatively propagated Chrysanthemum morifolium has been carried out by tissue culture.Explants derived from shoot-tip of three chrysant genotypes were used for the experiment. Shoot-tips taken from terminal branch and sterilized with 0.05% HgCb for 30 minutes and rinsed with distilled water three times.Shoot-tips were cultured on MS regeneration medium containing 1 mg/1 BAP and 0.02 mg/1 NAA.Shoot regeneration occurred 20 days after cultured.A number of tiny shoots were then transferred to fresh regeneration medium for 3 weeks and regenerated shoots were calculated.Results showed that plant genotypes had different response into plant regeneration. Genotype Ku-Ch had better response compared to other two genotypes which shown from number of plantlets produced by this genotype on the third sub-culture.
Co-Authors Ardjasa, Wayan Sabe Idawati Idawati Ita Dwimahyani M Lina S Slamet, S Wemay, J