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All Journal 3BIO: Journal of Biological Science, Technology and Management Journal of Tropical Chemistry Research and Education
Sriyati , Siti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Neuroscience

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Evaluation of Introductory Environmental Chemistry Course for Prospective Chemistry Teachers Using Context, Input and Process Models Widyaningsih, Mia; Nahadi, N; Mudzakir, Ahmad; Sriyati , Siti; Khoerunnisa, fitri
Journal of Tropical Chemistry Research and Education Vol. 6 No. 2 (2024)
Publisher : Chemistry Education Department, Faculty of Tarbiyah and Education, UIN Sunan Kalijaga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/jtcre.2024.62-05

Abstract

This study aims to evaluate the learning programme of the Introduction to Environmental Chemistry course of the Chemistry Education study programme in one of the public universities in Bandung City. This study needs to be evaluated and improved because there are still aspects that are not in accordance with 21st century learning as evidenced by document analysis and learning observations. The method used is a qualitative approach and the CIPP (Context, input, process, Product) evaluation model as a tool to evaluate and improve the lecture programme which focuses on the Context aspect in the form of document analysis of the Semester Learning Plan (SLP), the Input aspect in the form of lecturer interviews and the Process aspect in the form of learning observations. The results obtained from the analysis show that there are several findings that need to be evaluated due to the incompatibility of aspects in the SLP document update such as administrative completeness, the General Skills Aspect (GSA) 1 listed is not in accordance with the Context of Learning Outcomes (CLOs), based on interviews with teaching lecturers it is stated that problem-based learning (PBL) with the context of validated environmental issues in relevant theoretical content and in the aspect of the learning observation process it was found that there were aspects of learning practices that were not in accordance with the SLP document reference. The evaluation also highlighted the importance of updating and adjusting learning media to be more interactive and relevant to the context of environmental issues. The assessment of the last three years shows the potential to further develop programmes can to improve students' higher order thinking skills
In Silico Phylogenetic Analysis of Lamiaceae Based on ITS, matK, and rbcL DNA Barcodes Endlessa, Chayra; Hidayat , Topik; Sriyati , Siti
3BIO: Journal of Biological Science, Technology and Management Vol. 8 No. 1 (2026): Vol 8 No 1 (2026)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2026.8.1.4

Abstract

Lamiaceae, widely used as herbal medicine, is increasingly vulnerable to adulteration driven by market demand, compromising product safety and efficacy. Prevention is challenging due to morphological similarities; thus, DNA-based phylogenetics offer an alternative for accurate species authentication. However, Lamiaceae phylogenetics remain complicated by inconsistencies between morphological and DNA data. This study reconstructed Lamiaceae phylogeny using partial ITS, matK, and rbcL barcodes to evaluate their potential application in species authentication and adulteration prevention. Sequences for 52 species across 11 genera (Spathodea campanulata: outgroup) were obtained from NCBI GenBank, aligned, and trimmed. Four maximum parsimony (MP) trees were constructed in MEGA 11 (three single-barcode, one concatenated). The concatenated dataset was also analyzed by maximum likelihood (ML). Tree robustness was evaluated with bootstrapping, consistency index (CI), and retention index (RI). matK had the longest mean sequence (785.6 bp), rbcL the highest homology (83.5%), and ITS the most parsimony-informative sites (40.3%). MP trees exhibited moderate homoplasy (mean CI = 0.63) but strong synapomorphic signal (mean RI = 0.83). Individual barcodes produced similar genus groupings, yet misplaced several species. Concatenation corrected these positions across MP and ML trees, resolving six robust monophyletic clades (bootstrap >70%), broadly consistent with earlier phylogenies: Callicarpa; Scutellaria; Clerodendrum, Lamium, and Stachys; Salvia; Thymus, Origanum, and Mentha; Orthosiphon and Ocimum. Topological discrepancies with prior studies likely reflect differences in barcode choice and taxon sampling. Concatenated barcodes improved phylogenetic resolution in Lamiaceae, producing clades that identify potential adulterants and guide DNA marker development for species authentication and adulterant detection.
Co-Authors Ahmad Aminudin Endlessa, Chayra Fitri Khoerunnisa Nahadi Topik Hidayat Widyaningsih, Mia