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All Journal The Journal of Experimental Life Sciences (JELS) Jurnal SainHealth PHARMACOLOGY, MEDICAL REPORTS, ORTHOPEDIC, AND ILLNESS DETAILS (COMORBID) Svāsthya: Trends in General Medicine and Public Health
Harmoko, Deni
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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PERBANDINGAN UJI Treponema pallidum Haemagglutination Assay (TPHA) DAN IMUNOKROMATOGRAFI SEBAGAI DETEKSI SIFILIS di PUSKESMAS MEDAENG: COMPARISON OF Treponema pallidum Haemagglutination Assay (TPHA) TEST AND IMMUNOCHROMATOGRAPHY AS SYPHILIS DETECTION AT MEDAENG PUBLIC HEALTH CENTER Romadhon, Desi Fitriatur; Wijayanti, Christina Destri Wiwis; Sundari, Titik; Harmoko, Deni
Jurnal SainHealth Vol. 8 No. 2 (2024): September 2024
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v8i2.16861.65-69

Abstract

Syphilis is a sexually transmitted infection caused by the bacteria Treponema pallidum. Syphilis detection can be done using the Treponema pallidum Haemagglutination Assay (TPHA) method and immunochromatography. Treponema pallidum Haemaglutination Assay (TPHA) is a specific antibody test to detect Treponema pallidum but the process is long and expensive, compared to fast and relatively cheap immunochromatography. This study aimed to compare the Treponema pallidum Haemagglutination Assay (TPHA) method and immunochromatography as syphilis detection by looking at their positive and negative reaction percentage. The type of research used was descriptive-analytical observation. The results of the Treponema pallidum Haemagglutination Assay (TPHA) examination were positive for 10 samples (50%) and negative for 10 samples (50%). Meanwhile, positive immunochromatography was in 8 samples (40%) and negative for 12 samples (60%). It can be concluded that TPHA and immunochromatography have a different result. Keywords: syphilis, immunochromatography, TPHA, sensitivity, specificity
AKTIVITAS PROBIOTIK BAKTERI Enterococcus lactis SA8 ASAL SUSU KERBAU SUMBAWA (Bubalus bubalis): PROBIOTIC ACTIVITY OF Enterococcus lactis SA8 BACTERIA FROM SUMBAWA BUFFALO MILK (Bubalus bubalis) Harmoko, Deni; Ningrum, Rizka Kusuma; Pati, Andra Alvianto Umbu
Jurnal SainHealth Vol. 8 No. 2 (2024): September 2024
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v8i2.16905.70-75

Abstract

The most important quality of lactic acid bacteria as probiotics used for additives is tolerance to bile salts and low pH, which allows bacteria to survive, grow, and provide therapeutic effects in the digestive tract. Probiotics are living microorganisms that can provide optimal health effects when consumed in certain amounts, even exceeding general nutrition. Lactic acid bacteria are probiotic bacteria that can provide good health benefits when consumed regularly. The growth and production of lactic acid in LAB are influenced by pH and bile salts, this ability distinguishes between species of lactic acid bacteria. LAB must have The main physiological requirements of probiotic bacteria are their resistance and tolerance to low pH and bile salts. This study aims to test E. lactis SA8 bacteria from Sumbawa Buffalo milk to determine their probiotic activity through tests on pH resistance and their ability to live in bile salts. E. lactis SA8 bacteria have probiotic activity, which is indicated by their ability to withstand low pH, and the results of bile salt testing show the highest viability at a concentration of 0.3% with a density of 9.8x107 CFU/mL, which is a sign of probiotic bacteria.  Keywords: Probiotics, lactic acid bacteria, Enterococcus lactis SA8, pH and bile salt tests
Isolation and Screening of Lactic Acid Bacteria From Sumbawa Buffalo Milk (Bubalus bubalis) as Potential Starter Cultures Harmoko, Deni; Ardyati, Tri; Jatmiko, Yoga Dwi
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.03

Abstract

The Sumbawa buffalo (Bubalus bubalis) is one of the ruminant livestock in Indonesia that not only contributes to fulfilling meat requirements but also milk. Besides containing nutrients that are very beneficial for human health, buffalo milk is also a potential source of lactic acid bacteria (LAB) with technological and functional properties. Lactic acid bacteria have been utilized as starter cultures in various fermented products. This study aimed to isolate LAB from Sumbawa buffalo milk and to identify the potential isolate as a starter culture. The screening of LAB as a starter culture was based on some technological properties, including proteolytic activity, lipolytic activity, exopolysaccharide (EPS) production, antibacterial activity, antibiotic sensitivity, hemolytic activity, and acidification activity Data were analyzed statistically using one-way ANOVA and Tukey’s post hoc test at a 5% significance level. A total of 21 isolates were isolated from fresh buffalo milk, with a LAB total was 4.7x105 CFU.mL-1. All the isolates were characterized as Gram-positive with cocci-shaped. The SA8 isolate was selected as the most potential candidate as a starter culture because it has fulfilled the criteria such as the highest proteolytic activity, the lowest lipolytic activity, producing EPS, potential antagonistic activity against Bacillus cereus, Escherichia coli, and Salmonella Typhi, and sensitivity to cefazolin, intermediate to erythromycin and cinoxacin, non-pathogen, as well as the most rapid acidification activity. The SA8 isolate was identified as Enterococcus lactis with a similarity level of 99.99% towards strain BT159. This indigenous LAB was a potential starter culture of Sumbawa fermented buffalo milk to increase the diversification of products derived from buffalo milk. Keywords: Enterococcus lactis, lactic acid bacteria, technological properties, starter culture, Sumbawa buffalo milk.
Differences in Lactate Dehydrogenase (LDH) Levels in Packed Red Cells (PRC) with Various Storage Times Ersanto, Novi; Harmoko, Deni; Lukiyono, Yauwan Tobing
PHARMACOLOGY, MEDICAL REPORTS, ORTHOPEDIC, AND ILLNESS DETAILS Vol. 4 No. 2 (2025): APRIL
Publisher : Transpublika Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55047/comorbid.v4i2.1756

Abstract

This research seeks to analyze the storage duration effect on PRC (Packed Red Cell) quality by assessing changes in LDH (Lactate Dehydrogenase) levels on storage days 1, 10, 20, and 30. This research is an observational analytical study with a time series design. The research was conducted at the Blood Transfusion Installation of Dr. Soetomo General Hospital Surabaya as the sampling site and the Clinical Laboratory of the Faculty of Health Sciences, Maarif Hasyim Latif University, Sidoarjo as the place for measuring research variables. The research sample consisted of fifty PRC bags from voluntary donors. LDH levels were examined with a Semi-Automatic Chemistry Analyzer Sinnowa BS-3000P Photometer (Sinnowa Medical Science & Technology Co., Ltd., China) and measured on days 1, 10, 20, and 30. The research data were analyzed using SPSS 25 statistical software. Data normality was analyzed using the Shapiro-Wilk test. Comparison of PRC quality was analyzed using repeated measure ANOVA analysis (same object analysis) if the data were normally distributed, or the Freedman test if the data distribution was not normal. The findings revealed that there was an increase in mean LDH levels with increasing PRC storage duration over 30 days. Repeated Measure ANOVA test and the Post Hoc Bonferroni test for LDH levels showed that there were statistically significant differences with a significance value of p < 0.001 at all observation times. In conclusion, there are significant differences in LDH levels during Packed Red Cell (PRC) storage from day 1, day 10, day 20, and day 30.
The Isolation of mercury-resistant bacteria in the Brantas River, Malang, Indonesia Harmoko, Deni
Svāsthya: Trends in General Medicine and Public Health Vol. 2 No. 1 (2025): January 2025
Publisher : PT. Mega Science Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70347/svsthya.v2i1.77

Abstract

Mercury (Hg) is a toxic heavy metal that can originate from various sources, such as gold mining, the industrial sector, atmospheric deposition, and natural activities. Mercury contamination can cause liver, kidney, and enzyme system damage, as well as DNA damage. Because microorganisms are more sensitive to heavy metal stress than are animals and plants, the characteristics of microorganisms can be potential indicators of environmental health. This study aimed to isolate and identify mercury-resistant bacteria in the Brantas River, Malang as potential indicators of Hg pollution. Samples were isolated on nutrient agar (NA) media supplemented with 0.01 ppm HgCl₂ as a selective medium. The growing bacterial colonies were purified, and further resistance tests were carried out by adding HgCl₂ at concentrations of 0.03 ppm, 0.05 ppm, and 0.1 ppm. Bacterial isolates that survived at a concentration of 0.1 ppm HgCl₂ were then tested for sensitivity to concentrations of 0.15 ppm and 0.2 ppm HgCl₂. Bacterial isolation via HgCl₂ 0.01 ppm selective media produced 28 bacterial isolates. Further testing revealed that 19 isolates were resistant to 0.03 ppm HgCl₂, 13 isolates (0.05 ppm), and five isolates (0.1 ppm. Sensitivity tests on five bacterial isolates revealed that at a HgCl₂ concentration of 0.15 ppm, isolates RS118, RS1221, and RS1323 showed no growth inhibition, whereas at a concentration of 0.2 ppm, an inhibition zone formed for all five isolates. Although mercury-resistant bacteria were detected, this finding does not directly indicate the presence of mercury pollution in the river because bacterial resistance can be caused by various factors, including horizontal gene transfer between bacterial communities. Comprehensive testing of Hg concentrations in rivers is required to accurately determine the status of Hg pollution. This study highlights the importance of considering multiple biomarkers and environmental parameters alongside bacterial resistance patterns when assessing Hg contamination in riverine ecosystems.
Co-Authors Ersanto, Novi Ningrum, Rizka Kusuma Pati, Andra Alvianto Umbu Romadhon, Desi Fitriatur TITIK SUNDARI Tri Ardyati Wijayanti, Christina Destri Wiwis YOGA DWI JATMIKO