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All Journal Biota Jurnal Biologi Tropis Jurnal SainHealth
Ningrum, Rizka Kusuma
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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AKTIVITAS PROBIOTIK BAKTERI Enterococcus lactis SA8 ASAL SUSU KERBAU SUMBAWA (Bubalus bubalis): PROBIOTIC ACTIVITY OF Enterococcus lactis SA8 BACTERIA FROM SUMBAWA BUFFALO MILK (Bubalus bubalis) Harmoko, Deni; Ningrum, Rizka Kusuma; Pati, Andra Alvianto Umbu
Jurnal SainHealth Vol. 8 No. 2 (2024): September 2024
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v8i2.16905.70-75

Abstract

The most important quality of lactic acid bacteria as probiotics used for additives is tolerance to bile salts and low pH, which allows bacteria to survive, grow, and provide therapeutic effects in the digestive tract. Probiotics are living microorganisms that can provide optimal health effects when consumed in certain amounts, even exceeding general nutrition. Lactic acid bacteria are probiotic bacteria that can provide good health benefits when consumed regularly. The growth and production of lactic acid in LAB are influenced by pH and bile salts, this ability distinguishes between species of lactic acid bacteria. LAB must have The main physiological requirements of probiotic bacteria are their resistance and tolerance to low pH and bile salts. This study aims to test E. lactis SA8 bacteria from Sumbawa Buffalo milk to determine their probiotic activity through tests on pH resistance and their ability to live in bile salts. E. lactis SA8 bacteria have probiotic activity, which is indicated by their ability to withstand low pH, and the results of bile salt testing show the highest viability at a concentration of 0.3% with a density of 9.8x107 CFU/mL, which is a sign of probiotic bacteria.  Keywords: Probiotics, lactic acid bacteria, Enterococcus lactis SA8, pH and bile salt tests
Isolation of Lactic Acid Bacteria from California Papaya and Antibacterial Activity as a Potential Starter Culture Harmoko, Deni; Suryani, Elsa Mega; Ningrum, Rizka Kusuma
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10717

Abstract

Papaya fruit has many benefits for body health. Lactic acid bacteria can be used as a starter culture to produce more diverse foods (food diversification) and increase product shelf life. This study was conducted to obtain LAB isolates from California papaya that have potential as starter cultures. The methods in this study include the LAB isolation stage followed by the gram staining process, endospore staining, catalase test, hemolytic test, and antibacterial activity test using S. aurius and E. coli as indicator bacteria. Data were analyzed statistically using one-way ANOVA and Tukey’s post hoc test at 5% of significance level. The total count (TPC) of LAB in California papaya samples was 3.6x105 CFU/mL. The results of LAB isolation from California papaya obtained 14 LAB isolates, after going through the testing and screening stages obtained 8 LAB isolates that could potentially be used as starter culture candidates, namely isolates DPC1, DPC5, DPC6, DPC7, DPC10, DPC11, DPC12, DPC14. Isolate DPC6 is an isolate with the highest antibacterial activity in inhibiting the growth of S. aureus and E. coli bacteria, so it is a potential bacterial candidate that can be used as a starter culture.
Isolation and Identification of Endophytic Cellulolytic Bacteria in Syzygium aqueum Fruit Prasetya, Indra Adi Wira; Suryani, Elsa Mega; Ningrum, Rizka Kusuma
Biota Vol 11 No 2 (2025): Jurna Biota 2025
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v11i2.26424

Abstract

Some endophytic bacteria are known to have hydrolytic activity by producing hydrolase enzymes. Endophytic bacteria can be found in plant tissue. Exploration of endophytic cellulolytic bacteria in water apple (Syzygium aqueum) fruit has not been widely carried out, so this research is the first to be conducted. This study aimed to isolate, screen, and identify endophytic bacteria from water apple fruit that could produce cellulase enzymes. The research began with bacterial isolation. Then, cellulolytic screening was carried out by inoculating the isolates into a differential medium containing cellulose, Bushnell-Haas agar (BHA). The screening results were characterized and identified through 16S rDNA gene sequence analysis. The isolation results indicated that seven bacterial isolates were screened for cellulolytic activity by cultivating them on BHA medium. The screening revealed that one isolate encode CSZA2 had cellulolytic activity with a cellulolytic index of 0.87. Biochemical characterization and molecular identification of the CSZA2 isolate showed a 99.93% similarity to the Pseudomonas putida RTI2, a bacterium known for producing cellulase enzymes. The discovery of bacterial cellulolytic activity can be developed for biomass degradation, bioethanol production, and agricultural biotechnology.
Co-Authors Harmoko, Deni Pati, Andra Alvianto Umbu Prasetya, Indra Adi Wira Suryani, Elsa Mega