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All Journal SCIENCE TECH: Jurnal Ilmiah Ilmu Pengetahuan dan Teknologi Menara Ilmu Jurnal Biogenerasi
Wahyudi, Christosie Immanuel
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Agriculture, Biological Sciences & Forestry Chemistry Computer Science & IT Education Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Other

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Molecular Characterization on Vitellogenins (Vtg) of Grass Carp (Ctenopharyngodon idella) Nisa, Silmy Aulia Rufiatin; Nafisah, Nur Apriatun; Wahyudi, Christosie Immanuel
Menara Ilmu : Jurnal Penelitian dan Kajian Ilmiah Vol 19, No 2 (2025): Vol 19 No. 02 JANUARI 2025
Publisher : LPPM Universitas Muhammadiyah Sumatera Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31869/mi.v19i2.6175

Abstract

Ikan koan memiliki berbagai manfaat untuk kepentingan akuakultur karena mampu berperan sebagai agen biokontrol di perairan sekaligus sebagai sumber protein berkualitas tinggi sehingga berpotensi sebagai target pengembangan budidaya ikan. Upaya yang dapat dilakukan dalam mengembangkan budidaya perikanan adalah menghasilkan bibit unggul melalui manipulasi reproduksi. Penelitian ini dilakukan untuk memahami karakteristik molekuler serta biokimia dari protein Vtg sebagai salah satu protein yang berperan penting dalam oogenesis pada ikan koan, khusunya selama proses Vitelogenesis. Studi dilakukan secara in silico, antara lain analisis filogenetik pada ketiga jenis protein Vtg, analisis karakter fisikokimia dan prediksi domain serta situs fosforilasi. Hasil karakterisasi menunjukkan bahwa Ci-VtgAa dan Ci-VtgAb memiliki hubungan evolusioner yang lebih dekat dibanding dengan Ci-VtgC. Hal tersebut didukung dengan analisis prediksi domain protein yolk, dimana Ci-VtgC tidak memiliki domain Phosvitin (Pv). Hasil analisis karakter fisikokimia mengindikasikan bahwa protein Ci-VtgC cenderung bersifat asam namun lebih stabil dibandingkan Ci-VtgAa dan Ci-VtgAb. Ketiga protein memiliki ketahanan terhadap rentangan temperatur yang luas, memiliki interaksi yang baik dengan molekul air, serta komposisi asam amino didominasi oleh Leucine (Leu). Adanya situs fosforilasi dan O-glikosilasi pada ketiga protein mengindikasikan peran Ci-VtgAa, Ci-VtgAb, dan Ci-VtgC dalam transpor ion logam dan karbohidrat.Kata Kunci: ikan koan, betina, analisis protein, vitelogenesis
Artikel Review : Agen Pengendali Hayati Serangga Hama Penggerek Buah Kopi (Hypothenemus hampei Ferr.) pada Tanaman Kopi Wahyudi, Christosie Immanuel
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5328

Abstract

Coffee is an important commodity for the Indonesian economy, which is the third largest coffee producer in the world after Brazil and Vietnam. Coffee cultivation in Indonesia began in 1699. Coffee has become a widely known agricultural product and is expected to increase state revenues from exports. The types of coffee cultivated include Robusta, Arabica, and Liberica, with Robusta dominating production. Indonesia. Coffee farmers face problems in the form of decreased quality and quantity of production caused by pest attacks. One of the main pests is the coffee berry borer (CBO), known by the scientific name Hypothenemus hampei. This pest is difficult to control and can damage coffee fruit and beans, lowering its market price. The distribution of CBO is almost evenly distributed throughout Indonesia, with varying levels of damage in various regions, such as 64% in Lampung and 80% in Papua. This pest has a complete life cycle with four stages: eggs, larvae, pupae, and adults. Females can produce 50 eggs, which hatch in 5-9 days. The CBO life cycle takes about 25 days, with varying lifespans for females and males. PBKo attacks occur on coffee berries aged 8 weeks until harvest, which can damage coffee beans and reduce yields. The age of the coffee plant also affects the quantity of fruit produced and the distribution of pests. Coffee plants that are not exposed to direct sunlight have higher temperatures and humidity, supporting the growth of PBKo. PBKo pest control can be done biologically/biological control. Some PBKo pest control agents include predators, parasitoids, entomopagenic fungi, entomopagenic bacteria, and entomopagenic nematodes.
Artikel Review : Agen Pengendali Hayati Serangga Hama Penggerek Buah Kopi (Hypothenemus hampei Ferr.) pada Tanaman Kopi Wahyudi, Christosie Immanuel
Jurnal Biogenerasi Vol. 10 No. 2 (2025): Volume 10 no 2 periode februari - september 2025 ( continues)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i2.5328

Abstract

Coffee is an important commodity for the Indonesian economy, which is the third largest coffee producer in the world after Brazil and Vietnam. Coffee cultivation in Indonesia began in 1699. Coffee has become a widely known agricultural product and is expected to increase state revenues from exports. The types of coffee cultivated include Robusta, Arabica, and Liberica, with Robusta dominating production. Indonesia. Coffee farmers face problems in the form of decreased quality and quantity of production caused by pest attacks. One of the main pests is the coffee berry borer (CBO), known by the scientific name Hypothenemus hampei. This pest is difficult to control and can damage coffee fruit and beans, lowering its market price. The distribution of CBO is almost evenly distributed throughout Indonesia, with varying levels of damage in various regions, such as 64% in Lampung and 80% in Papua. This pest has a complete life cycle with four stages: eggs, larvae, pupae, and adults. Females can produce 50 eggs, which hatch in 5-9 days. The CBO life cycle takes about 25 days, with varying lifespans for females and males. PBKo attacks occur on coffee berries aged 8 weeks until harvest, which can damage coffee beans and reduce yields. The age of the coffee plant also affects the quantity of fruit produced and the distribution of pests. Coffee plants that are not exposed to direct sunlight have higher temperatures and humidity, supporting the growth of PBKo. PBKo pest control can be done biologically/biological control. Some PBKo pest control agents include predators, parasitoids, entomopagenic fungi, entomopagenic bacteria, and entomopagenic nematodes.
Keanekaragaman dan Potensial Musuh Alami Koloni Tetragonula leaviceps (Hymenoptera: Apidae: Meliponinae) Hashifah, Fathimah Nurfithri; Indraswari Suhri, Andi Gita Maulidyah; Nafisah, Nur Apriatun; Wahyudi, Christosie Immanuel
Science Tech: Jurnal Ilmu Pengetahuan dan Teknologi Vol 11 No 1 (2025): Februari
Publisher : Universitas Sarjanawiyata Tamansiswa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30738/st.vol11.no1.a17830

Abstract

This study discusses the diversity of natural enemies of Tetragonula leaviceps (stingless) bees. The natural enemies obtained act as predators, honey thieves, and nest destroyers (intruders). This research was conducted from September to November 2020 at Caping Merapi Plantation Sleman, Yogyakarta. This research uses the random sampling method in collecting species. All natural enemies that have been identified were obtained as many as 11 species of natural enemies of Tetragonula leaviceps bees. These species were identified from 628 individuals consisting of Insecta, Arachnida, and Reptilia classes. All classes are potential predators, but those that act as honey thieves and nest destroyers are only from the Formicidae family. The results of this study are expected to increase knowledge in stingless bee farming so that it can further maximise nest boxes, nest areas or habitats, and supporting plants as a source of bee food.
Co-Authors Hashifah, Fathimah Nurfithri Indraswari Suhri, Andi Gita Maulidyah Nafisah, Nur Apriatun Nisa, Silmy Aulia Rufiatin