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All Journal Wahana Matematika dan Sains Journal of Marine and Coastal Science
Ni Putu Dian Pertiwi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Identifikasi Spesies Ikan Lutjanus yang Didaratkan di PPI Kedonganan Berdasarkan Karakter Morfologi, Morfometri dan DNA Barcoding Natalia, Kadek Desika; Ni Putu Dian Pertiwi
Wahana Matematika dan Sains: Jurnal Matematika, Sains, dan Pembelajarannya Vol. 18 No. 3 (2024): Desember 2024
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/wms.v18i3.87683

Abstract

Identifikasi spesies sangat penting untuk inventarisasi ikan dengan nilai ekonomis tinggi di Indonesia. Penelitian ini bertujuan untuk mengidentifikasi spesies ikan Lutjanus di Kedonganan, Bali, menggunakan metode morfologi, morfometrik, dan DNA barcoding dengan marka control region mitokondria (mtDNA). Sampel dianalisis melalui pengamatan morfologi secara visual, pengukuran 23 karakter morfometrik, serta sekuensing DNA yang diikuti dengan analisis filogenetik. Hasil pengamatan morfologi menunjukkan ciri-ciri spesifik seperti tubuh berwarna kuning terang dengan lima garis biru keabu-abuan, yang mengidentifikasi spesimen sebagai Lutjanus quinquelineatus. Hasil identifikasi dengan DNA barcoding menunjukkan sampel yang dikoleksi sebagai spesies Lutjanus quinquelineatus dengan tingkat kemiripan sebesar 97,67%. Jarak genetik intraspesifik menunjukkan nilai sebesar 2,37%. Kombinasi data morfologi, morfometrik, dan molekuler mengonfirmasi identifikasi spesimen secara akurat. Oleh karena itu, penelitian ini diharapkan dapat menambah informasi genetik tentang keanekaragaman spesies ikan Lutjanus yang terdapat di Indonesia, yang tercatat dalam database genetik secara keseluruhan. Kata kunci: Bali, identifikasi, ikan Lutjanus, keanekaragaman
Genetic Diversity of Unhatched Sea Turtle Eggs in Buleleng, Bali Indonesia Hatchery Based on The Mitochondrial Control region Locus Immanuel Pratama; Ni Putu Dian Pertiwi; I Made Oka Riawan; Ni Luh Astria Yusmalinda; Andrianus Sembiring; Luh Putu Candra Apriliani; Ni Komang Rossa Sri Savitri; Srie Marhaeni Julyasih, Ketut; Jafron Syah, Moh; Rita Rachmawati
Journal of Marine and Coastal Science Vol. 14 No. 3 (2025): SEPTEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v14i3.77095

Abstract

Sea turtles are protected animals that are threatened with extinction due to natural and anthropogenic factors such as coastal erosion and illegal trade. This study aims to analyze the genetic diversity of sea turtles in Buleleng using mitochondrial DNA from unhatched egg samples. Samples were collected from two nests at several breeding sites to determine genetic variation within a single conservation area. Molecular analysis was conducted on the control region locus with a sequence length of 869 bp. The results of the identification showed that the samples originated from three species: green sea turtles (Chelonia mydas), olive ridley sea turtles (Lepidochelys olivacea), and hawksbill sea turtles (Eretmochelys imbricata), with sequence similarities of 99.44%–99.77%. The phylogenetic tree formed four clades with bootstrap values of 79–100%, supporting the classification and kinship relationships between species. Low intraspecific genetic distance (0–0.2%) and interspecific genetic distance (16.4–21.4%) indicate high similarity within a species and significant differences between species. The results of this study can be used to strengthen effective genetics-based sea turtle conservation programs in the Buleleng region of North Bali.
Co-Authors Andrianus Sembiring I Made Oka Riawan Immanuel Pratama Ketut Srie Marhaeni Julyasih Luh Putu Candra Apriliani Natalia, Kadek Desika Ni Komang Rossa Sri Savitri Ni Luh Astria Yusmalinda Rita Rachmawati