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All Journal Jurnal Biologi Tropis Journal of Marine and Coastal Science
Luh Putu Candra Apriliani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Genetic Diversity of Unhatched Sea Turtle Eggs in Buleleng, Bali Indonesia Hatchery Based on The Mitochondrial Control region Locus Immanuel Pratama; Ni Putu Dian Pertiwi; I Made Oka Riawan; Ni Luh Astria Yusmalinda; Andrianus Sembiring; Luh Putu Candra Apriliani; Ni Komang Rossa Sri Savitri; Srie Marhaeni Julyasih, Ketut; Jafron Syah, Moh; Rita Rachmawati
Journal of Marine and Coastal Science Vol. 14 No. 3 (2025): SEPTEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v14i3.77095

Abstract

Sea turtles are protected animals that are threatened with extinction due to natural and anthropogenic factors such as coastal erosion and illegal trade. This study aims to analyze the genetic diversity of sea turtles in Buleleng using mitochondrial DNA from unhatched egg samples. Samples were collected from two nests at several breeding sites to determine genetic variation within a single conservation area. Molecular analysis was conducted on the control region locus with a sequence length of 869 bp. The results of the identification showed that the samples originated from three species: green sea turtles (Chelonia mydas), olive ridley sea turtles (Lepidochelys olivacea), and hawksbill sea turtles (Eretmochelys imbricata), with sequence similarities of 99.44%–99.77%. The phylogenetic tree formed four clades with bootstrap values of 79–100%, supporting the classification and kinship relationships between species. Low intraspecific genetic distance (0–0.2%) and interspecific genetic distance (16.4–21.4%) indicate high similarity within a species and significant differences between species. The results of this study can be used to strengthen effective genetics-based sea turtle conservation programs in the Buleleng region of North Bali.
Genetic Diversity and Species Identification of Unhatched Sea Turtle Eggs from Southern Bali Hatcheries Dewana, I Gede Jose; Pertiwi, Ni Putu Dian; Yuliastuti, Yuliastuti; Yusmalinda, Ni Luh Astria; Luh Putu Candra Apriliani; Savitri, Ni Komang Rossa Sri; Rachmawati, Rita; Sembiring, Andrianus
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.9897

Abstract

Genetic data of sea turtles in Southern Bali remains limited as most previous studies rely on adult or live samples, while unhatched eggs are rarely utilized as a genetic resource. This study aims to identify the species and genetic diversity of unhatched sea turtle eggs from conservation sites in Southern Bali using mitochondrial control region. A total of six non-viable eggs were analyzed through DNA extraction, PCR, sequencing, and data analysis using MEGA 11, NCBI BLAST, and DnaSP v6. The identification results revealed that all samples belonged to the species of Lepidochelys olivacea (olive ridley turtle), with sequence similarity ranging from 99.54% to 100% and query coverage of 89% to 99% compared to reference data from GenBank. Phylogenetic analysis showed that all samples clustered within the same clade, with intraspecific genetic distances ranging from 0 to 0.008. Genetic diversity analysis revealed a haplotype diversity (Hd) of 0.86667 and nucleotide diversity (π) of 0.00306, with a total of four distinct haplotypes and six polymorphic sites. These findings indicate that unhatched sea turtle eggs still contain viable genetic material for analysis and suggest that Lepidochelys olivacea is the dominant nesting species found in Southern Bali. This study carries important implications for advancing conservation policy, enhancing habitat protection, minimizing inbreeding risks, and strengthening the use of species identification through forensic genetics.
Co-Authors Andrianus Sembiring Andrianus Sembiring, Andrianus Dewana, I Gede Jose I Made Oka Riawan Immanuel Pratama Ketut Srie Marhaeni Julyasih Ni Komang Rossa Sri Savitri Ni Luh Astria Yusmalinda Ni Putu Dian Pertiwi Ni Putu Dian Pertiwi, Ni Putu Dian Rita Rachmawati Rita Rachmawati, Rita Savitri, Ni Komang Rossa Sri Yuliastuti Yuliastuti Yusmalinda, Ni Luh Astria