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All Journal Prisma Sains: Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram BIO-SAINS | Jurnal Ilmiah Biologi
Anissa, Ratih Khairul
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Mathematics Physics Other

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KEANEKARAGAMAN DAN KELIMPAHAN CAPUNG (ODONATA: ANISOPTERA) DI LAPANGAN WATU GAJAH TUBAN Sonia, Shela; Agistiana Azzahra, Athiyya Nur; Anissa, Ratih Khairul; Jamilah, Yaquta Maziyatin; Rahayu, Dwi Anggorowati
BIO-SAINS : Jurnal Ilmiah Biologi Vol 2 No 1 (2022)
Publisher : Fakultas Sains dan Teknologi, Universitas Islam As-Syafi'iyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34005/bio-sains.v1i2.1755

Abstract

Odonata merupakan ordo dengan tingkat keanekaragaman dan kelimpahan terbesar di antara filum Arthropoda lain tetapi banyak yang belum teridentifikasi. Capung menyukai habitat daerah terbuka hijau, utamanya rerumputan. Penelitian ini bertujuan menganalisis keanekaragaman dan kelimpahan insekta capung (Odonata: Anisoptera) di Lapangan Watu Gajah Kecamatan Semanding Kabupaten Tuban. Lapangan Watu Gajah termasuk ruang terbuka hijau yang mendukung kelangsungan hidup insekta, salah satunya capung. Kondisi iklim Lapangan Watu Gajah dengan rata-rata suhu 290C, ketinggian 40 mdpl, kecepatan angin 13 km/h, curah hujan 1,9 mm, kelembaban 70,33%, dan intensitas cahaya 4259,33 lux. Penelitian dengan metode jelajah (Cruising method) menggunakan jala insekta dan teknik hand sampling pada 3 titik area. Berdasarkan hasil penelitian diperoleh 6 spesies capung. Analisis data dilakukan dengan menggunakan rumus kelimpahan relatif, indeks keanekaragaman Shannon-Winner, dan indeks dominansi Shimpson. Hasil perhitungan indeks keanekaragaman capung tergolong rendah, yaitu 1,57 dan indeks dominansi 0,226 termasuk dalam kategori sedang. Lapangan Watu Gajah menjadi habitat ideal yang sesuai untuk kelangsungan hidup capung (Odonata: Anisoptera).
Identification of Promising Glyphosate-Degrading Bacteria Isolated from the Rhizosphere of Local Chili Pepper Plantation Lisdiana, Lisa; Maulida, Khilma Ziyadatur Rizka; Anissa, Ratih Khairul; Arista, Anggi Maulia
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Vol 13, No 1: January 2025
Publisher : IKIP Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/j-ps.v13i1.14449

Abstract

The glyphosate’s continuous application in agricultural fields has caused adverse environmental effects. Utilizing indigenous microorganisms as glyphosate-degrading agents can be an effective and eco-friendly solution. Isolate Cf2, obtained from a local chili pepper plantation in Indonesia, grew as a co-dominant isolate in media with 50 ppm glyphosate, indicating its potential as glyphosate-degrading bacteria. However, this isolate cannot be applied yet because it has not been identified. Identification can provide initial insights into the safety of indigenous isolates for ecological application. Thus, this study aims to identify isolate Cf2 by utilizing the 16s rRNA gene sequence as the genetic marker. The data was compared to the database using the web-based BLAST2 program (version 2.13.0). Then, the evolutionary relationship of this isolate with its closest relatives was assessed through phylogenetic tree reconstruction. The results demonstrate that the isolate Cf2 has a sequence similarity of 98.88% with Bacillus subtilis. The phylogenetic tree reconstruction further indicates that isolate Cf2 forms a monophyletic clade with this species. Hence, it can be concluded that isolate Cf2 is indeed B. subtilis. This study is the first report of glyphosate-degrading B. subtilis isolated from chili pepper plantations in Indonesia, offering a new insight into bioremediation strategies.
Co-Authors Agistiana Azzahra, Athiyya Nur Arista, Anggi Maulia Dwi Anggorowati Rahayu Jamilah, Yaquta Maziyatin Lisa Lisdiana Maulida, Khilma Ziyadatur Rizka Sonia, Shela