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All Journal HAYATI Journal of Biosciences JTSL (Jurnal Tanah dan Sumberdaya Lahan) Jurnal Agrotek Tropika
Christy Nur Cahyani
Fakultas Pertanian, Universitas Lampung
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science

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Crude Oil Biodegradation Potential using Acinetobacter baumannii CYA20 and Bacillus subtilis CYA27 from the Bekasi Coast, Indonesia Christy Nur Cahyani; Andes Ismayana; Mohamad Yani
HAYATI Journal of Biosciences Vol. 29 No. 5 (2022): September 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.5.701-711

Abstract

The pollution of coastal areas caused by oil spills is an environmental issue that needs further attention. Crude oil contains persistent organic pollutants (POPs) that are difficult to degrade. This study aimed to isolate bacterial strains capable of degrading crude oil from the Bekasi coast through bacterial isolate characterization and crude oil biodegradation tests using a crude oil-enriched microcosm model. Two strains with higher TPH degradation values were selected among the isolated bacteria, namely CYA20 and CYA27, which specifically showed values of 46% and 66%, respectively. These strains were tolerant to NaCl concentrations of up to 4% and 12%, respectively, and they were also tolerant to pH values ranging from 5 to 9 and temperatures between 10°C and 50°C. Both strains were shown to utilize POPs, such as phenanthrene and fluorene, produce biosurfactants, and exhibited an emulsification activity in paraffin ranging from 0.373 to 0.533. Phylogenetic analyses identified these bacterial strains as Acinetobacter baumannii CYA20 and Bacillus subtilis CYA27, respectively. The results of this study indicate that these isolates could be developed as biodegradation agents for the bioremediation of crude oil-contaminated environments.
POTENSI PEMANFAATAN PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) DAN BERBAGAI MEDIA TANAM TERHADAP POPULASI MIKROBA TANAH SERTA PERTUMBUHAN DAN PRODUKSI KENTANG Christy Nur Cahyani; Yulia Nuraini; Al Gamal Pratomo
Jurnal Tanah dan Sumberdaya Lahan Vol. 5 No. 2 (2018)
Publisher : Departemen Tanah, Fakultas Pertanian, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.508 KB)

Abstract

The availability of high quality potato seeds in Indonesia is only 7.4% including imports. Improvement of soil fertility is done with PGPR and various growing media, so that soil quality increases and can increase potato productivity. This research used a split plot design with twelve treatments with three replicates. Treatments tested were P0M1 (without PGPR + Husk Charcoal : Cocopeat (1:1)), P1M1 (10 ml/l PGPR + Husk Charcoal : Cocopeat (1:1)), P2M1 (20 mL L-1 PGPR + Husk Charcoal : Coco peat (1:1)), P0M2 (without PGPR + Soil : Husk Charcoal (1:1)), P1M2 (10 mL L-1 PGPR + Soil : Husk Charcoal (1:1)), P2M2 (20 mL L-1 PGPR + Soil : Husk Charcoal (1:1)), P0M3 (without PGPR + Soil : Husk Charcoal : Cocopeat (1:1:1)), P1M3 (10 mL L-1 PGPR + Soil : Husk Charcoal : Cocopeat (1:1:1)), P2M3 (20 mL L-1 PGPR + Soil : Husk Charcoal : Cocopeat (1:1:1)), P0M4 (without PGPR + Tanah), P1M4 (10 mL L-1 PGPR + Soil), P2M4 (20 mL L-1 PGPR + Soil). PGPR and growing media yielded an interaction with total N-l in P2M2 treatment, with the highest value of 1,02%,. The highest total P was in the P2M2 treatment (0,35%). Nitrogen fixing bacteria were observed in P2M2 treatment with the highest value of 77,25 x 105 cfu mL-1. Phosphate solubilizing bacteria were observed in P2M2 treatment with the highest value of 45 x 105 cfu mL-1. Plant height and dry weight of plants in P2M2 with the highest values of 12,99 cm and 3,61 gram, respectively. The highest yield of potato (20 tubers plant-1) was in the P2M2 treatment.
Phytophthora palmivora Relationship Analysis on ITS rDNA Primers Using MEGA11 Software (NCBI DNA Sequencing Library Study) Rani Yosilia; Auliana Afandi; Ade Lenty Hoya; Christy Nur Cahyani; Ayaka Hieno
Jurnal Agrotek Tropika Vol 12, No 4 (2024): JURNAL AGROTEK TROPIKA VOL 12, NOVEMBER 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i4.9532

Abstract

Phylogenetic study of Phytophthora palmivora species utilizing nucleotide sequences of rDNA ITS and genes was conducted to evaluate the cladistics and evolution of kinship among P. palmivora species in order to support genetic diversity and linkages and also compare with sequences of mitochondrial COX1. The analysis included 61 nucleotide sequences of P. palmivora rDNA ITS and one outgroup as well as 56 nucleotides sequences of COX1 retrieved from the NCBI GeneBank, which were aligned and trimmed using MEGA 11 software. The Kimura-2 parameter model and Maximum-Likelihood approach were used to create a monophyletic tree with four major clades. The percentage of GC content in DNA sequences was less than 50%, influencing genetic diversity and adaptation throughout evolutionary time periods. Temperature, humidity, rainfall, soil moisture, and seasonal fluctuations all work together to generate optimal climatic and weather conditions for the establishment and development of Phytophthora palmivora in tropical climates.
Co-Authors Ade Lenty Hoya Al Gamal Pratomo Auliana Afandi Ayaka Hieno Moh. Yani Muhammad Romli dan Suprihatin Andes Ismayana Rani Yosilia Yulia Nuraini