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ISOLATION AND CHARACTERISTIC OF NITROGEN-FIXING BACTERIA AND PHOSPHATE-SOLUBILIZING BACTERIA FROM SOIL HIGH IN MERCURY IN TAILINGS AND COMPOST AREAS OF ARTISANAL GOLD MINE Nuraini, Yulia; Arfarita, Novi; Siswanto, Bambang
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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The research was conducted at Brawijaya University and West Nusa Tenggara, from March 2013 to October 2013. The tailings areas of the gold mine contains high mercury (Hg) as much as 1,090 ppm, and living microbes (resistance) exist in a small number in such a condition. Microbial P solvents encountered came from the genus Bacillus with a population of 23 x 103 cfu g-1 and N-fixing bacteria encountered are of the genus Bacillus, with a population of 4 x 103 cfu g-1. Identification of species using Becton Dickinson Phoenix test, both species belong to Bacillus megaterium and Bacillus pumilus. While the waste corn and peanuts that has been composted for 4 weeks acquired P-solubilizing bacteria, such as Enterobacter, Bacillus and Pseudomonas, and N-fixing bacteria found was Pseudomonas and Azotobacter. While testing the activity and antagonism of N-fixing and P-solubilizing bacteria, the result on agar media did not show antagonism in its growth. Bacillus pumilus and Bacillus megaterium effective at 5 % molasses medium with the number of 0.15 x 1012 on seven days of incubation.Â Keywords: N-fixing bacteria, P-solubilizing bacteria, mercury, phytoremediation

Merah dan Pengaruhnya terhadap Biokimia Tanah Pada Percobaan Pot Menggunakan Tanah Ultisol Antonius, Sarjiya; Sahputra, Rozy Dwi; Nuraini, Yulia; Dewi, Tirta Kumala
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTUltisol dominated about 25% of the total land area in Indonesia. It has the potential to be used as agricultural land and shallot plants have a considerable prospect to be cultivated in ultisol soil. Ultisol generally has not been handled properly. It needs some specific treatments due to the low nutrient contents, caused by the intensive leaching process. One of the effort to overcome the soil quality problems that occur in ultisol soils (high soil acidity, average pH <4.50, high Al saturation, and low macronutrient content such as P, K, Ca, Mg, and organic material content) is treated by the using the soil conditioner such as the supplying of organic materials in the form of compost and biochar. In this work, the biological organic fertilizer was also applied to increase microorganism activity in the soil. The experimental design used in this work was completely random design which has 8 combinations of soil treatment, compost, biochar and bio-organic fertilizer. Soil parameters measured were pH, C-Organic, P-Available, total bacterial population, soil respiration, and phosphomonoesterase enzyme activity. The agronomy parameters of shallot were also measured. The results showed that treatment of compost, biological organic fertilizer, and biochar have significant effect to increase soil microorganism activity in the form of total population of bacteria, soil respiration, P-Available and pH. The treatment also had a significant effect on supporting plant height at 2 and 6 MST, number of leaves at 2 MST, wet weight and dry weight of tubers. Keywords: biochar, bio-organic fertilizer, soil microorganism activity, shallot, ultisol

MERAH DAN PENGARUHNYA TERHADAP BIOKIMIA TANAH PADA PERCOBAAN POT MENGGUNAKAN TANAH ULTISOL Antonius, Sarjiya; Sahputra, Rozy Dwi; Nuraini, Yulia; Dewi, Tirta Kumala
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3744

ABSTRACTUltisol dominated about 25% of the total land area in Indonesia. It has the potential to be used as agricultural land and shallot plants have a considerable prospect to be cultivated in ultisol soil. Ultisol generally has not been handled properly. It needs some specific treatments due to the low nutrient contents, caused by the intensive leaching process. One of the effort to overcome the soil quality problems that occur in ultisol soils (high soil acidity, average pH <4.50, high Al saturation, and low macronutrient content such as P, K, Ca, Mg, and organic material content) is treated by the using the soil conditioner such as the supplying of organic materials in the form of compost and biochar. In this work, the biological organic fertilizer was also applied to increase microorganism activity in the soil. The experimental design used in this work was completely random design which has 8 combinations of soil treatment, compost, biochar and bio-organic fertilizer. Soil parameters measured were pH, C-Organic, P-Available, total bacterial population, soil respiration, and phosphomonoesterase enzyme activity. The agronomy parameters of shallot were also measured. The results showed that treatment of compost, biological organic fertilizer, and biochar have significant effect to increase soil microorganism activity in the form of total population of bacteria, soil respiration, P-Available and pH. The treatment also had a significant effect on supporting plant height at 2 and 6 MST, number of leaves at 2 MST, wet weight and dry weight of tubers. Keywords: biochar, bio-organic fertilizer, soil microorganism activity, shallot, ultisol

Phytotoxicity of coal fly ash on plant growth and heavy metal uptake by plant in an acid soil Nisma Ula Shoumi Rahmawati; Novi Rahmawati Sutopo; Yulia Nuraini; Eko Handayanto
Journal of Degraded and Mining Lands Management Vol 7, No 3 (2020)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2020.073.2233

Considering the content of various elements in coal fly ash (CFA), it has the potential to be used as an additional source of nutrients in the soil. However, the use of CFA for agriculture is still debated because CFA contains several metal elements. The purpose of this study was to study the CFA phytotoxicity to seed germination and metal uptake by plants on acid soil. The first experiment was the study of the effect of extracts of various CFA and soil mixtures (5% - 45% CFA) on the germination of mustard seeds. The second study was a CFA phytotoxicity test for plant growth and metal uptake by the mustard plant. Results of the first experiment showed that the application of CFA had no significant effect on mustard seed germination, but had a significant effect on radicle length. CFA application increased the uptake of Pb and Cu by plant along with the increase in the proportion of CFA in the CFA + soil mixture. The contents of Cu and Pb in the mustard plant due to CFA application up to 30% were still below the threshold levels of Cu (36 ppm) and Pb (2 ppm).

Utilization of indigenous phosphate-solubilizing bacteria to optimize the use of coal fly ash for increasing available-P in an Ultisol Budi Purnomo; Novi Rahmawati Sutopo; Yulia Nuraini
Journal of Degraded and Mining Lands Management Vol 8, No 4 (2021)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2021.084.2937

Coal fly ash (CFA) is a coal-burning by-product containing macro and micronutrients, and it is the potential material for improving available P in Ultisols. Phosphate-solubilizing bacteria (PSB) play a role in phosphorus solubilization. This study aimed at elucidating the potential use of phosphate-solubilizing bacteria to optimize the use of coal fly ash for increasing soil available P. This study was conducted in two stages, namely isolation of indigenous PSB from an Ultisol and application of the PSB and CFA to improve soil available P. Five indigenous PSB isolated from the soil had the ability to dissolve phosphate. Isolate B5 could dissolve 9.89 ppm P and had a 99.57% closeness to Pseudomonas stutzeri. The application of 20 and 40 t CFA ha-1 increased the soil pH by 4.2% and 7.2%, respectively. Increasing the dose of CFA decreased the content of available P by 50.6%. However, the combination of PSB and 20 t CFA ha-1 increased soil available P, plant growth, plant dry biomass, and P-uptake by plant.

Effect of application compost and vermicompost from market waste on soil chemical properties and plant growth Syarifinnur Syarifinnur; Yulia Nuraini; Budi Prasetya
Journal of Degraded and Mining Lands Management Vol 9, No 2 (2022)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2022.092.3379

This study was conducted to determine the effect of compost and vermicompost from market organic waste on the soil chemical properties and the growth of maize. The treatments tested were three doses of compost (2.5, 5, and 10 t/ha), three doses of vermicompost (2.5, 5, and 10 t/ha), and one control (without compost or vermicompost). At the time of harvest (10 weeks after planting), maize shoot dry weight, root dry weight, cob length, cob diameter, cob with husk, and cob dry weight, as well as the soil chemical properties organic carbon (C), total nitrogen (N), total phosphorus (P), available P, total potassium (K), and pH were observed. Maize plant height, leaf number, and stem diameter were observed at 2, 4, 6, and 8 weeks after planting. The results showed that the application of compost and vermicompost significantly affected soil chemical properties and the yield of maize. The application of 10 t vermicompost/ha resulted in the highest yield of maize and highest increase of soil organic carbon, total phosphorus available phosphorus, total potassium, and pH by 7.21%, 112.41%, 287.44%, 85.44% and 17.58%, respectively. The application of 10 t compost/ha resulted in the highest increase of soil total N by 44%.

The possible use of coal fly ash and phosphate-solubilizing fungi for improving the availability of P and plant growth in acid soil Retno Wilujeng; Gusti Irya Ichriani; F Fahrunsyah; Yulia Nuraini; Eko Handayanto
Journal of Degraded and Mining Lands Management Vol 8, No 1 (2020)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2020.081.2471

The availability of P in acid soils may be raised through the application of phosphate solubilizing fungi (PSF). Coal fly ash (CFA) that has a high pH and contains a relatively high P may also be used to raise the availability of P in acid sois. The purpose of this study was to explore the possible use of CFA and PSF in a biochar-compost carrier to improve the availability of P and plant growth in acid soil. Combined applications of two PSF isolates (Aspergillus oryzae = F1, and Neosartorya fischeri = F2) carried in three levels of biochar-compost (80% biochar + 20% compost = B1, 70% biochar + 30% compost = B2, and 60% biochar + 40% compost =B3), and two doses of CFA (60 t/ha = C1, and 80 t/ha = C2) were tested in this study through two experiments. The results of experiment 1 (laboratory experiment) showed that the application of N. fischeri carried in 70% biochar + 30% compost combined with 80 t CFA/ha (F2B2C2 treatment) significantly increased the available P more than other treatments. In comparison with control, the increase of soil available P content ranged from 13% in the F1B1C1 treatment (A. oryzae in 80% biochar + 20% compost combined with 60 t CFA/ha) to 101% in the F2B2C2 treatment (N.fischeri in 70% biochar + 30% compost combined with 80 t CFA/ha). The results of experiment 2 (glasshouse experiment) showed that the highest dry weight of maize shoot was obtained by the F2B2C2 treatment that increased 123% compared to control. The highest P uptake by maize was obtained by the F2B2C2 treatment (N. fischeri in 70% biochar + 30% compost combined with 80 t CFA/ha).

Aplikasi Rabuk Kandang Sapi Dan Pupuk Nitrogen Untuk Memperbaiki Kualitas Entisol Bagi Jagung Manis Ika Apriwulandari; Yulia Nuraini; Zaenal Kusuma
The Indonesian Green Technology Journal Vol 1, No 2 (2012)
Publisher : Program Pascasarjana Universitas Brawijaya

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Tujuan penelitian untuk mengetahui pengaruh pemberian kotoran sapi terhadap sifat kimia tanah, pencucian nitrat dan pertumbuhan tanaman jagung manis. Aplikasi pupuk kandang sapi diduga dapat memperbaiki sifat kimia tanah, mengurangi pencucian nitrat dan memperbaiki pertumbuhan jagung manis. Penelitian di rumah kaca, menggunakan Rancangan Acak Lengkap (RAL) sederhana. Perlakuan dosis pupuk nitrogen : 0, 100, 200, 300, 400, 500 kgN /ha. Perlakuan pupuk kandang sapi: tanpa pupuk kandang, pupuk kandnag dosis 39.72 ton/ha (setara 76.39 g/pot). Hasil penelitian menunjukkan bahwa pemberian pupuk nitrogen yang dikombinasikan dengan pupuk kandang sapi memperbaiki sifat kimia tanah, menurunkan pencucian nitrat rata-rata sebesar 13.68 % dibandingkan kontrol, dan memperbaiki pertumbuhan tanaman jagung manis. Kombinasi perlakuan K1N2 ( Nitrogen 200 kg/ha + BO 76,39 g/pot (setara 39.72 ton/ha) menghasilkan serapan N tanaman paling tinggi (383.76 mg/tanaman) dan efisiensi penggunaan nitrogen sebesar 69.00 kg/ha dibandingkan perlakuan lainnya. Kata kunci: pupuk kandang sapi, nitrogen, jagung manis

The use of pineapple liquid waste and cow dung compost to improve the availability of soil N, P, and K and growth of pineapple plant in an Ultisol of Central Lampung Winih Sekaringtyas Ramadhani; Yulia Nuraini
Journal of Degraded and Mining Lands Management Vol 6, No 1 (2018)
Publisher : Brawijaya University

A study that was aimed to elucidate the effect of adding pineapple liquid waste and cow dung compost in increasing the availability of soil N, P, K, contents of N, P, K in pineapple leaves and growth of pineapple plant in an Ultisol was conducted in Central Lampung. The study consisted of two steps, i.e. incubation of pineapple liquid waste with cow dung compost in the soil, and planting pineapple by adding pineapple liquid waste and cow dung compost. Treatments tested in experiment 1 (laboratory incubation experiment) were combinations of pineapple liquid waste (L) and cow dung compost (K). Changes of soil chemical characteristics were observed at 15, 30, 45, and 60 days. Three best treatments of experiment 1 were selected for the experiment 2 (plant growth experiment in a glasshouse). Two control treatments were included in experiment 2. N, P, and K contents in pineapple leaves as well as and pineapple root length were observed at 30 and 60 days. Results of the first experiment showed that addition of pineapple liquid waste and cow dung compost significantly increased soil pH, availability of soil N, P, and K compared to controls at 15, 30, 45, and 60 days. Addition of 20 t cow dung compost/ha and 20,000 L pineapple liquid waste/ha (K3L3 treatment) resulted in higher values of soil pH, total N, available P, and available K than the control. Results of the second experiment showed that the addition of pineapple liquid waste and cow dung compost gave no significant effect on the growth of pineapple plant, but the addition of 20 t cow dung compost/ha and 10,000 L pineapple liquid waste/ha (K3L2 treatment) gave optimum growth compared to other treatments at 60 days. The K3L2 treatment also had higher pineapple leaf nutrient content, compared to control.

ISOLATION AND CHARACTERISTIC OF NITROGEN-FIXING BACTERIA AND PHOSPHATE-SOLUBILIZING BACTERIA FROM SOIL HIGH IN MERCURY IN TAILINGS AND COMPOST AREAS OF ARTISANAL GOLD MINE Yulia Nuraini; Novi Arfarita; Bambang Siswanto
AGRIVITA, Journal of Agricultural Science Vol 37, No 1 (2015): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i1.422

The research was conducted at Brawijaya University and West Nusa Tenggara, from March 2013 to October 2013. The tailings areas of the gold mine contains high mercury (Hg) as much as 1,090 ppm, and living microbes (resistance) exist in a small number in such a condition. Microbial P solvents encountered came from the genus Bacillus with a population of 23 x 103 cfu g-1 and N-fixing bacteria encountered are of the genus Bacillus, with a population of 4 x 103 cfu g-1. Identification of species using Becton Dickinson Phoenix test, both species belong to Bacillus megaterium and Bacillus pumilus. While the waste corn and peanuts that has been composted for 4 weeks acquired P-solubilizing bacteria, such as Enterobacter, Bacillus and Pseudomonas, and N-fixing bacteria found was Pseudomonas and Azotobacter. While testing the activity and antagonism of N-fixing and P-solubilizing bacteria, the result on agar media did not show antagonism in its growth. Bacillus pumilus and Bacillus megaterium effective at 5 % molasses medium with the number of 0.15 x 1012 on seven days of incubation.

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