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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA)
Dyah Kinasih Wuragil
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Characterization of TPO Enzyme and its Immunogenecity in New Zealand White Rabbits (Oryctolagus cuniculus) Devi Ramadani; Andreas Budi Wijaya; Arie Srihardyastutie; Dyah Kinasih Wuragil; Rulli Rosandi; Achmad Rudijanto; Aulanni'am Aulanni'am
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9539

Abstract

Hypothyroidism is characterized by inadequate production of thyroid hormones, chiefly thyroxine (T4) and triiodothyronine (T3). Thyroid peroxidase (TPO), is an enzyme and crucial for producing thyroid hormones, and also serves as a primary autoantigen in autoimmune thyroid conditions, such as Hashimoto's thyroiditis and Graves' disease. The existence of anti-thyroid peroxidase antibodies (anti-TPO) indicates an autoimmune response in which the immune system TPO which is normally recognized as part of the body (self), begins to be identified by the immune system as a something foreign (non-self). This study aimed to characterize, measure TPO activity, and evaluate the immunogenicity of TPO protein derived from patient serum and its ability to induce TPO antibody production in New Zealand White Rabbits. TPO protein isolation was achieved through SDS-PAGE and confirmed by western blot technique have molecular weight of 52 kDa. In samples from normal patients, the average TPO enzyme activity was 31.34 U/mL, while in hypothyroid patients, the average activity increased to 49.46 U/mL. TPO polyclonal antibody production in rabbits indicated its immunogenicity, peaking in week eight post-immunization, with an ELISA absorbance of 0.555. Dot blot analysis further confirmed anti-TPO sensitivity at various dilutions, anti-TPO has a high sensitivity at 1:80 dilution still detects TPO in sera patient.
Co-Authors Achmad Rudijanto Andreas Budi Wijaya Arie Srihardyastutie Aulanni'am Aulanni'am Devi Ramadani Rulli Rosandi