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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA)
Dyah Kinasih Wuragil
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Characterization of TPO Enzyme and its Immunogenecity in New Zealand White Rabbits (Oryctolagus cuniculus) Devi Ramadani; Andreas Budi Wijaya; Arie Srihardyastutie; Dyah Kinasih Wuragil; Rulli Rosandi; Achmad Rudijanto; Aulanni'am Aulanni'am
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9539

Abstract

Hypothyroidism is characterized by inadequate production of thyroid hormones, chiefly thyroxine (T4) and triiodothyronine (T3). Thyroid peroxidase (TPO), is an enzyme and crucial for producing thyroid hormones, and also serves as a primary autoantigen in autoimmune thyroid conditions, such as Hashimoto's thyroiditis and Graves' disease. The existence of anti-thyroid peroxidase antibodies (anti-TPO) indicates an autoimmune response in which the immune system TPO which is normally recognized as part of the body (self), begins to be identified by the immune system as a something foreign (non-self). This study aimed to characterize, measure TPO activity, and evaluate the immunogenicity of TPO protein derived from patient serum and its ability to induce TPO antibody production in New Zealand White Rabbits. TPO protein isolation was achieved through SDS-PAGE and confirmed by western blot technique have molecular weight of 52 kDa. In samples from normal patients, the average TPO enzyme activity was 31.34 U/mL, while in hypothyroid patients, the average activity increased to 49.46 U/mL. TPO polyclonal antibody production in rabbits indicated its immunogenicity, peaking in week eight post-immunization, with an ELISA absorbance of 0.555. Dot blot analysis further confirmed anti-TPO sensitivity at various dilutions, anti-TPO has a high sensitivity at 1:80 dilution still detects TPO in sera patient.
Antihypertensive Effect of Rice Bran Derived Amino Acids Through Modulation of iNOS Expression and Renal Histopathology in DOCA Salt Induced Hypertensive Rats Muhammad Fikri Nur; Devi Ramadani; Muh Fikry Baharuddin; Almas Dwi Khairana; Saidun Fiddaroini; Akhmad Sabarudin; Anna Safitri; Wibi Riawan; Dyah Kinasih Wuragil; Hilman Nurmahdi; Aulanni'am Aulanni'am
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.13491

Abstract

Hypertension is a major global health concern and a leading risk factor for cardiovascular mortality. Conventional treatment with ACE inhibitors like captopril is effective but often causes adverse effects including cough, dizziness, and renal impairment. This study evaluated the antihypertensive effects of rice bran in DOCA-salt-induced hypertensive rats. In vitro analysis revealed 45.58% soluble fiber and rich amino acid content, including 2.121 mg L-Arginine, a nitric oxide precursor promoting vasodilation. Male Wistar rats were divided into five groups: negative control, positive control, captopril treatment (5 mg/kg BW), and rice bran supplementation at 1% and 2% of 25 g feed. The 2% rice bran group significantly (p < 0.05) reduced renal iNOS expression by 88.32% compared to hypertensive controls, surpassing captopril's 22.96% reduction. Histopathological examination revealed notable renal protection with complete absence of fatty degeneration and minimal glomerular inflammation in the 2% rice bran group. These findings demonstrate potent antihypertensive activity through ACE inhibition, enhanced nitric oxide bioavailability, and mitigation of oxidative and inflammatory stress, establishing rice bran as a promising natural therapeutic alternative for hypertension management
Co-Authors Achmad Rudijanto Akhmad Sabarudin Almas Dwi Khairana Andreas Budi Wijaya Arie Srihardyastutie Aulanni'am Aulanni'am Devi Ramadani Hilman Nurmahdi Muh Fikry Baharuddin Muhammad Fikri Nur Rulli Rosandi Safitri, Anna Saidun Fiddaroini Wibi Riawan