Article Per Year (5 Year)
p-Index From 2021 - 2026
0.659
P-Index
This Author published in this journals
All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Dyah Kinasih Wuragil
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Materials Science & Nanotechnology Physics Social Sciences

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Characterization of TPO Enzyme and its Immunogenecity in New Zealand White Rabbits (Oryctolagus cuniculus) Devi Ramadani; Andreas Budi Wijaya; Arie Srihardyastutie; Dyah Kinasih Wuragil; Rulli Rosandi; Achmad Rudijanto; Aulanni'am Aulanni'am
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9539

Abstract

Hypothyroidism is characterized by inadequate production of thyroid hormones, chiefly thyroxine (T4) and triiodothyronine (T3). Thyroid peroxidase (TPO), is an enzyme and crucial for producing thyroid hormones, and also serves as a primary autoantigen in autoimmune thyroid conditions, such as Hashimoto's thyroiditis and Graves' disease. The existence of anti-thyroid peroxidase antibodies (anti-TPO) indicates an autoimmune response in which the immune system TPO which is normally recognized as part of the body (self), begins to be identified by the immune system as a something foreign (non-self). This study aimed to characterize, measure TPO activity, and evaluate the immunogenicity of TPO protein derived from patient serum and its ability to induce TPO antibody production in New Zealand White Rabbits. TPO protein isolation was achieved through SDS-PAGE and confirmed by western blot technique have molecular weight of 52 kDa. In samples from normal patients, the average TPO enzyme activity was 31.34 U/mL, while in hypothyroid patients, the average activity increased to 49.46 U/mL. TPO polyclonal antibody production in rabbits indicated its immunogenicity, peaking in week eight post-immunization, with an ELISA absorbance of 0.555. Dot blot analysis further confirmed anti-TPO sensitivity at various dilutions, anti-TPO has a high sensitivity at 1:80 dilution still detects TPO in sera patient.
Antihypertensive Effect of Rice Bran Derived Amino Acids Through Modulation of iNOS Expression and Renal Histopathology in DOCA Salt Induced Hypertensive Rats Muhammad Fikri Nur; Devi Ramadani; Muh Fikry Baharuddin; Almas Dwi Khairana; Saidun Fiddaroini; Akhmad Sabarudin; Anna Safitri; Wibi Riawan; Dyah Kinasih Wuragil; Hilman Nurmahdi; Aulanni'am Aulanni'am
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.13491

Abstract

Hypertension is a major global health concern and a leading risk factor for cardiovascular mortality. Conventional treatment with ACE inhibitors like captopril is effective but often causes adverse effects including cough, dizziness, and renal impairment. This study evaluated the antihypertensive effects of rice bran in DOCA-salt-induced hypertensive rats. In vitro analysis revealed 45.58% soluble fiber and rich amino acid content, including 2.121 mg L-Arginine, a nitric oxide precursor promoting vasodilation. Male Wistar rats were divided into five groups: negative control, positive control, captopril treatment (5 mg/kg BW), and rice bran supplementation at 1% and 2% of 25 g feed. The 2% rice bran group significantly (p < 0.05) reduced renal iNOS expression by 88.32% compared to hypertensive controls, surpassing captopril's 22.96% reduction. Histopathological examination revealed notable renal protection with complete absence of fatty degeneration and minimal glomerular inflammation in the 2% rice bran group. These findings demonstrate potent antihypertensive activity through ACE inhibition, enhanced nitric oxide bioavailability, and mitigation of oxidative and inflammatory stress, establishing rice bran as a promising natural therapeutic alternative for hypertension management
Betatrophin: A promising biomarker for metabolic syndrome and diabetes mellitus risk screening in teenagers Hendra Susanto; Aulanni’am Aulanni’am; Dyah Kinasih Wuragil; Ahmad Taufiq; Sunaryono Sunaryono; Jerry Dwi Trijoyo Purnomo; Dyah Ika Krisnawati; Moch Sholeh
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in Biomarkers, Therapeutics, and Probiotics: Recent Updates in Medical L
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6028

Abstract

Metabolic syndrome (MetS) and diabetes mellitus (DM) have become primary concerns worldwide, especially among the younger population. The Indonesian Boarding School model (IBS/Boarding School) is a large education system with a significant number of pupils (teenagers) and has the potential to become a center for metabolic disease, particularly among teenagers, due to their daily intake. This study aimed to provide a baseline screening for MetS and the risk of DM development in Boarding School teenagers. During this observational study, 90 healthy serological samples were obtained from senior and junior high school students. The circulating level of betatrophin was measured using a human betatrophin ELISA kit. Additionally, metabolic syndrome and DM screening data were analyzed using a rapid reverse-flow immunochromatography kit for 65 kDa glutamic acid decarboxylase (GAD65). Among the 90 healthy students, a high prevalence of GAD65 was observed, indicating a potential risk factor for metabolic diseases. Furthermore, higher serum betatrophin levels were observed in the samples. The circulating level of betatrophin was found to have a significant correlation with age, gender, body mass index (BMI), systolic blood pressure (SBP), fasting blood glucose (FBG), sleeping duration, and duration of stay at Boarding School (p < 0.05). Betatrophin emerged as a potential predictor of BMI, SBP, and FBG in students (p < 0.05). Both betatrophin and GAD65 have shown promise as future biomarkers, opening up a new avenue for assessing metabolic syndrome and the risk of DM. This underscores the importance of future programs in Boarding Schools focusing on MetS and DM prevention management, making the audience feel the significance of their work in addressing these pressing health issues.
Co-Authors Achmad Rudijanto Ahmad Taufiq Akhmad Sabarudin Almas Dwi Khairana Andreas Budi Wijaya Arie Srihardyastutie Aulanni'am Aulanni'am Aulanni’am Aulanni’am Devi Ramadani Dyah Ika Krisnawati Hendra Susanto Hilman Nurmahdi Jerry Dwi Trijoyo Purnomo Moch Sholeh Muh Fikry Baharuddin Muhammad Fikri Nur Rulli Rosandi Safitri, Anna Saidun Fiddaroini Sunaryono Wibi Riawan