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All Journal Journal on Education Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya
Vindi Annisa Rahmah
Universitas Adiwangsa Jambi
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education

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Potensi Aktivitas Antioksidan Fraksi Etanol Rotan Jernang Daemonorops Draco Vindi Annisa Rahmah; Nafisah Amri; Becek Hamisah
Journal on Education Vol. 7 No. 2 (2025): Journal on Education: Volume 7 Nomor 2 Tahun 2025 In Progress (Januari-Februari
Publisher : Mathematics Education Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joe.v7i2.8107

Abstract

Jernang rattan (Daemonorops draco) is used traditionally as treatment for various health problems. One of them is degenerative diseases caused by free radicals,such as cancer. A natural alternative that able to overcome this disease needs to be developed. Scientifically, jernang rattan is known to have variety of secondary metabolites such as flavonoids,terpenoids,and alkaloids that cause abundant pharmacological activity in it. This makes jernang rattan to be potential as an antioxidant or anticancer compound. Research began with the extraction of compounds using maceration methods with ethanol solvents. Yield of the thick extract obtained was 9,912%. Phytochemical analysis was carried out to determine the content of compounds in extract. It results that extract showed the presence of flavonoid,triterpenoid and alkaloid compounds. Vacuum column chromatography(KVC) is then carried out to simplify the compounds contained in extracts. From this stage 5 combined fractions were obtained which were then tested for antioxidant (DPPH method). The test results of antioxidant activity on the ethanol extract gave an IC50 value of 212,235. Meanwhile, the fraction shows better IC50 value of 32,807.
SKRINING POTENSI EKSTRAK KULIT JANTUNG PISANG KEPOK (Musa paradisiaca Linn.) SEBAGAI ZAT ANTIBAKTERI Vindi Annisa Rahmah; Agung Kurniawan; Widya Twiny Rizki; Siti Marwah Lestari; Chindiana Khutami
Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya Vol. 7 No. 2 (2025): Literasi Artikel Penelitian Kimia
Publisher : Program Studi Kimia, Fakultas MIPA, Universitas PGRI Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/jc.v7i2.6086

Abstract

Abstract Indonesian Food and Drug Authority or BPOM no longer recommends the use of synthetic preservatives due to their toxic properties and potential carcinogenic risks. One of the natural ingredients that is potential as a natural preservative is the peel of kepok banana blossom (Musa paradisiaca Linn.), which is known to contain secondary metabolites with microbial activity, including steroids, terpenoids, saponins, tanning, alkaloid, flavonoids, and phenols. This study aim to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in Staphylococcus aureus bacteria. The peel extract of kepok banana blossom (Musa paradisiaca Linn.) was obtained by maceration with a mixture of 96% ethanol: 3% Citric Acid (85:15) solvent. Concentration of extract varied from 12.5%; 25%; 50%; until 100%, it is used for antibacterial activity test in which aquadest as a negative control and Chloramphenicol as a positive control. The Minimum Inhibitory Concentration (MIC) test was conducted using the liquid macrodilution method and the Minimum Bactericidal Concentration (MBC) test was conducted using the spread method. The Minimum Inhibitory Concentration (MIC) value was obtained at a concentration of 50% and the Minimum Bactericidal Concentration (MBC) value was obtained at a concentration of 100%. The peel extract of Kepok banana blossom (Musa paradisiaca Linn.) with a concentration of 50% was able to inhibit the growth of Staphylococcus aureus bacteria and with a concentration of 100% was able to kill Staphylococcus aureus bacteria. Keywords: kepok banana blossom peel extract, natural preservatives, antibacterial acitivity test Abstrak Badan Obat dan Makanan atau BPOM telah menyarankan untuk menghindari penggunaan bahan pengawet sintesis karena cenderung toksi dan karsinogenik. Salah satu bahan alam yang berpotensi sebagai pengawet alami yaitu kulit jantung pisang kepok (Musa paradisiaca Linn.), yang diketahui mengandung metabolit sekunder yang berfungsi sebagai senyawa antimikroba diantaranya steroid, terpenoid, saponin, tanin, alkaloid, flavonoid dan fenol. Penelitian ini bertujuan untuk menentukan Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) pada bakteri Staphylococcus aureus. Ekstrak kulit jantung pisang (Musa paradisiaca Linn.) diperoleh dengan cara maserasi dengan pelarut yang terdiri dari campuran etanol 96% : Asam Sitrat 3% (85:15). Variasi konsentrasi ekstrak dibuat untuk pengujian aktivitas antibakteri dari 12,5%; 25%; 50%; hingga 100%, aquades sebagai kontrol negatif dan Kloramfenikol sebagai kontrol positif. Uji Konsentrasi Hambat Minimum (KHM) dilakukan dengan metode makrodilusi cair dan uji Konsentrasi Bunuh Minimum (KBM) dilakukan dengan metode sebar (spread). Nilai Konsentrasi Hambat Minimum (KHM) didapatkan pada konsentrasi 50% dan nilai Konsentrasi Bunuh Minimum (KBM) didapatkan pada konsentrasi 100%. Berdasarkan hasil penelitian ini, ekstrak kulit jantung pisang (Musa paradisiaca Linn.) dengan konsentrasi 50% diketahui mampu menghambat pertumbuhan bakteri Staphylococcus aureus dan dengan konsentrasi 100% mampu membunuh bakteri Staphylococcus aureus. Keywords: ekstrak kulit jantung pisang, pengawet alami, uji aktivitas antibakteri
Co-Authors Agung Kurniawan Becek Hamisah Chindiana Khutami Nafisah Amri Siti Marwah Lestari Widya Twiny Rizki