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All Journal Biology, Medicine, & Natural Product Chemistry Unesa Journal of Chemistry
Ridhwan, Alverdha Elvarheta Rasseta
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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PENGARUH PENAMBAHAN ENZIM PAPAIN DAN ENZIM BROMELIN TERHADAP HIDROLISAT PROTEIN DARI AMPAS KELAPA Ridhwan, Alverdha Elvarheta Rasseta; Herdyastuti, Nuniek
Unesa Journal of Chemistry Vol. 13 No. 1 (2024): Vol 13 No 1 (2024)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia

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Abstract

Coconut dregs waste has potential to be used as protein hydrolysate with addition of papain enzyme and bromelain enzyme. The study aimed to analyze the effect of enzyme concentration and hydrolysis time on coconut pulp protein hydrolysate based on yield, protein content, and degree of hydrolysis. The enzyme concentration used variations of 1, 3, 5, 6, and 7% (b/v) which were hydrolyzed for 240 minutes. Determination of protein content was done by Bradford method and measurement of hydrolysis degree by SN-TCA method. The results showed the best protein hydrolysate in addition of papain enzyme at 7% concentration with a yield of 24.70%, protein content of 0.03450 mg/mL, and degree of hydrolysis of 48.04%. These results were greater than addition of 7% concentration of bromelain enzyme with a yield of 18.46%, protein content of 0.03067 mg/mL, and degree of hydrolysis of 42.30%. The variation of hydrolysis time was 150, 180, 210, 240, and 270 minutes with 7% enzyme concentration for each enzyme. The results showed that the best hydrolysis time was 270 minutes with papain enzyme which produced 23.44% yield, 0.03550 mg/mL protein content, and 65.36% hydrolysis degree. These results are greater than the hydrolysis time of 270 minutes with bromelain enzyme producing a yield of 17.06%, protein content of 0.03360 mg/mL, and degree of hydrolysis of 50.20%. This study showed that protein hydrolysate with the addition of papain enzyme showed better results than the addition of bromelain enzyme.
Formulation and Standardization of Face Wash Using Watermelon Rind Extract (Citrullus lanatus) Wijaya, Dika Putra; Rahmawati, Khofifatul; Bachtiar, Achmad Yusuf; Ridhwan, Alverdha Elvarheta Rasseta; Rahmah, Ayu Alfianita; Taufikurohmah, Titik; Danar, Danar
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1569-1578

Abstract

The purpose of this study was to determine the utilization of watermelon rind waste as an active ingredient in the manufacture of liquid soap preparations and to obtain the best formulation of watermelon rind extract liquid soap that is physically stable. Watermelon rind waste contains many nutritious substances, such as antioxidants, that are beneficial for skin health but have not been widely utilized. This study utilizes citrulline compounds in watermelon rind, which act as antioxidants and potential vasodilators, to be used as active ingredients in the manufacture of bath soap preparations with the aim of removing dirt and stimulating blood circulation. Research method includes the preparation of thick watermelon rind extract, the preparation of salt solution, and the preparation of liquid soap preparation formulas with varying concentrations of watermelon rind extract. Evaluation includes an organoleptic test, pH, viscosity, foam height, foam stability, homogeneity, and spreadability to obtain a liquid soap formula with stable physical properties. Result showed that watermelon rind extract has the potential to be an active ingredient in liquid soap preparations in order to utilize waste and provide benefits for skin health.
Co-Authors Bachtiar, Achmad Yusuf Danar, Danar Nuniek Herdyastuti Rahmah, Ayu Alfianita Rahmawati, Khofifatul Titik Taufikurohmah Wijaya, Dika Putra