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All Journal Best Journal (Biology Education, Sains and Technology) PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat
Ayuni, Rieke Dwi
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Diesel Fuel Degradation Activity of Hydrocarbon-Degrading Bacteria from Nusantara Fisheries Port Brondong Lamongan Mahbubillah, M. Ainul; Tamam, Muhammad Badrut; Ayuni, Rieke Dwi; Savitri, Nynda Ayu Nadira; Ramadani, Aisyah Hadi
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.10887

Abstract

The Nusantara Fisheries Port (Pelabuhan Perikanan Nusantara - PPN) in Brondong, Lamongan Regency, is a port with high ship activities. This has led to hydrocarbon pollution in the form of diesel fuel and oil in the waters of PPN Brondong. The initial effort to address this pollution is by isolating and characterizing hydrocarbon-degrading bacteria present in these waters. This research is a strategic step in managing petroleum hydrocarbon spills in the area through bioremediation processes, either via bioaugmentation or biostimulation for further study. The research method involves isolating hydrocarbon-degrading bacteria from the waters of PPN Brondong using the spread plate isolation method. Different colony characteristics are selected for the purification of bacterial isolates. The obtained bacterial isolates are then subjected to hydrocarbon degradation activity by culturing them in a minimal medium containing diesel fuel. The degradation activity is measured using total petroleum hydrocarbon (TPH) testing. The results of this study yielded 7 different isolates from the fuel oil-contaminated sites in PPN Brondong. Culturing in a minimal medium containing diesel fuel yielded results showing that all isolates possess the ability to degrade hydrocarbons, as evidenced by the reduction in TPH.
BUILDING THE CAPACITY OF ORGANIC FARMERS IN PRIJEKNGABLAK VILLAGE FOR INDEPENDENT PRODUCTION OF BIOPESTICIDES AND LIQUID ORGANIC FERTILIZERS Mahbubillah, M. Ainul; Redjeki, Endah Sri; Khakim, Arsanul; Ayuni, Rieke Dwi; Rohmah, Laila Ainur
PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat Vol 4 No 1 (2024): Desember
Publisher : Fakultas Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/pakdemas.v4i1.329

Abstract

In 2012, Prijekngablak Village, Karanggeneng, Lamongan, faced crop failure due to an uncontrollable pest outbreak. This prompted the Rame Gawe II Farmers' Group to initiate organic farming, despite various social challenges. In 2022, the village government supported these efforts, enabling the community to produce liquid organic fertilizer (POC) and bio-pesticides. However, obstacles such as low public trust, poor institutional coordination, and inadequate laboratory facilities persisted. The Community Service team addressed these issues through focus group discussions (FGDs), facility improvements, laboratory management enhancements, and training on producing and applying POC and bio-pesticides. FGDs revealed that 54.3% of farmers had a strong understanding of organic farming, 62.9% moderately understood its benefits, 51.4% had practical experience, and 77.1% were willing to adopt it. Laboratory improvements and SOP implementation improved production performance. Key products included POC from water hyacinths, bio-pesticides containing Beauveria bassiana and Trichoderma sp., and PGPR isolated from local bamboo roots. Their application enhanced plant health and reduced pest and disease attacks, showcasing the potential of organic farming in overcoming agricultural challenges in the village.
Bioactif and Bioactivity Test of Moringa (Moringa sp.), Starfruit Wuluh (Averrhoa bilimbi), and Keres (Muntingia calabura) Latifah, Indah Ttriwahyuni; Solekha, Rofiatun; Haibaturrahma, Nur Fatimah Azzahra; Wulandari, Ayu Dewi; Ayuni, Rieke Dwi; Nisa, Salasun; Alfanani, Syafiq; Ramadhan, Muhammad Syahrur; Khakim, Arsanul
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.10729

Abstract

This study evaluated the bioactivity of Moringa plant extracts (Moringa oleifera), Star Fruit Wuluh (Averrhoa bilimbi), and Keres (Muntingia calabura) on antioxidant and antibacterial activities. Extraction was carried out using the maceration method with a 96% ethanol solvent. The antioxidant test was carried out using the DPPH method (2,2-diphenyl-1- picrylhydrazyl), while the antibacterial test was carried out on Staphylococcus aureus bacteria using the well diffusion method. The results showed that Moringa extract had a strong linear relationship between concentration and antioxidant activity, while Star Fruit and Keres extracts showed low antioxidant activity with IC50 values above 100 ppm, indicating weak activity. In the antibacterial test, Star Fruit extract produced a significant inhibition zone, although it was classified as a weak response. These findings suggest that all three plants have potential as sources of bioactive compounds, although optimal efficacy requires optimization of extraction and concentration methods. Further research is needed to identify the main active compounds and explore their mechanisms of action.
Co-Authors Aisyah Hadi Ramadani Alfanani, Syafiq Haibaturrahma, Nur Fatimah Azzahra Khakim, Arsanul Laila Ainur Rohmah Latifah, Indah Ttriwahyuni Mahbubillah, M. Ainul Nisa, Salasun Ramadhan, Muhammad Syahrur Redjeki, Endah Sri Rofiatun Solekha Savitri, Nynda Ayu Nadira Tamam, Muhammad Badrut Wulandari, Ayu Dewi