Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal Pharmaciana: Jurnal Kefarmasian Journal of Halal Science and Research
Mohamed, Farahidah
Unknown Affiliation
Medicine & Pharmacology Other

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
In silico and in vitro studies of flavanoid content of Uncaria gambir Roxb stem extract on antidiabetic activity Fawaid, Ainun; Mahfudh, Nurkhasanah; Utami, Dwi; Mohamed, Farahidah
Pharmaciana Vol. 15 No. 2 (2025): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v15i2.30765

Abstract

Bajakah Kalalawit (Uncaria gambir Roxb.) is a typical plant of the Dayak tribe of Kalimantan, Indonesia. This study aims to evaluate the antidiabetic potential of Bajakah Kalalawit stem extract through in silico and in vitro approaches. In silico analysis was conducted to identify the interaction of bioactive compounds in 96% ethanol extract of Bajakah Kalalawit stems with the alpha-glucosidase enzyme (PDB ID : 5NN8), which is the main target in the treatment of diabetes. The results of molecular simulations showed that 2 bioactive compounds of the flavonoid group, namely Bikisocoumarin (-8.25 kcal/mol) and Pinostrobin (-5.81 kcal/mol) contained in 96% ethanol extract of Bajakah Kalalawit stems have significant inhibitory potential against the enzyme. Furthermore, in vitro tests were conducted to assess the inhibitory activity against the alpha-glucosidase enzyme and its effect on reducing blood glucose levels. The experimental results showed that 96% ethanol extract of Bajakah Kalalawit stems was able to significantly (ρ < 0.05) inhibit alpha-glucosidase activity (IC50 = 0.296 ± 0.004 μg/mL). This finding indicates that Bajakah Kalalawit has the potential as a source of active antidiabetic ingredients that can be further developed as an alternative therapy for diabetes management.
Development of ethanol analysis in cough syrup medicine by colorimetric method Sultan, Siti Fatimah; Susanti, Hari; Mohamed, Farahidah; Salamah, Nina; Farida, Verda
Journal of Halal Science and Research Vol. 6 No. 1 (2025): February
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jhsr.v6i1.12217

Abstract

Cough syrup is a pharmaceutical preparation often containing ethanol as a solvent. However, the presence of ethanol in the drug can cause safety issues. This study aims to develop and validate a colorimetric method for determining ethanol in cough syrup. Cough syrup samples from different brands were analyzed using the method. The Visible spectrophotometric method uses the oxidation reaction of ethanol with potassium dichromate in an acidic condition. Method validation was conducted, including linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ). The results showed this method had good linearity (r > 0.99) with a concentration range of 0.48 – 5.76 % (b/v). UV-Vis spectrophotometric method showed good precision and accuracy with SD = 0.0014, RSD = 0.4046 %, and % recovery = 99.99 %, while LOD = 0.1074 % and LOQ = 0.3253 %. This study concludes that the colorimetric method can be used as a valid and complementary method for analyzing ethanol content in cough syrup, providing important information for producers and consumers regarding product safety.   Keywords: Analytical method validation, Cough syrup, Ethanol, Spectrophotometry.
Co-Authors Fawaid, Ainun Hari Susanti Muhammad Yusron Maulana El-Yunusi Nina Salamah Nurkhasanah Mahfudh Sultan, Siti Fatimah Verda Farida