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Effect of Temperature on Microplastic Degradation in Soil Environment Abah, Moses Adondua; Olawale, Otitoju; Timothy, Mgbede; Timothy, Nwali Chukwuebuka; Oyibo, Okpanachi Nuhu; Okpanachi, Victor; Yola, Anna; Uchechukwu, Ukoh Daniel; Ifeanyi, Onyedinma Emmanuel; Owei, Joy Ebimoboere; Abimbola, Adeyemi Bashirat; Najeeb, Aliyu Olamilekan; Chinenye, Chinweuba Rachael; Egwolo, Fueta Loveth; Iheanacho, Chinedu Christian; Edoka, Oduh Solomon
Asian Journal of Science, Technology, Engineering, and Art Vol 2 No 5 (2024): Asian Journal of Science, Technology, Engineering, and Art
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstea.v2i5.3797

Abstract

The contamination of soil caused by the degradation of plastics introduced through disposal has been increasing globally. Researchers have reported that exposure to microplastics, the degradative product of plastic materials triggers a “wide variety of toxic insults”. The present study aimed at investigating the effect of temperature on microplastic degradation in soil environment. Soil samples were collected from Federal University Wukari farm, air-dried and sieved to obtain fine particles. They were arranged in three groups; one served as the control (Group 1), containing no shredded microplastics, whereas microplastics were shredded in Group 2 and Group 3 soil samples. They were all arranged in triplicates. The control group was kept at room temperature for 14 days whereas Group 2 was exposed to sunlight for 14 days. Group 3 was exposed to sunlight for 7 days. The results obtained from this study revealed the presence of the following microplastic types in Group 2 and 3 soil samples: polyamide, polystyrene, polypropylene, polyethylene, ethylene glycol, terephthatic acid, acrylic acid and polyester. The highest level of total detectable microplastics were associated with Group 3 soil samples (59314.95±808.35), followed by Group 2 soil samples (56022±1352.14) and lastly Group 1 (control) soil samples (32703.51±649.99) respectively. The result also revealed polyamide to be the most abundant microplastic present in all the assayed soil samples: Group 3 (48977.99±1071.61), Group 2 (52204.46±582.03) and Group 1 (28022.08±425.28) whereas the least microplastic present in all assayed soil samples was Terephthatic acid shown as thus: Group 3 (393.69±17.44) > Group 2 (369.36±28.11) > Group 3 (211.32±14.77) respectively. Overall, the result revealed that soil samples exposed to sunlight for a period of 7 days (Group 3) had the highest level of individually detected microplastics followed by soil samples exposed to sunlight for 14 days (Group 2). The control group clearly showed the least levels of individually detected microplastics. The study revealed that microplastics upon exposure to UV rays from sun light could undergo degradation yielding several intermediates which may be either more or less harmful. The study also revealed that the longer the exposure of a microplastic material to UV rays, the higher the degradation rate and the more intermediates associated with the microplastic material will be yielded. On the other hand, the shorter the exposure time of a microplastic material to UV rays, the lesser the degradation rate and less intermediates associated with the microplastic material will be yielded.
Molecular Basis of Chemotherapy and Radiotherapy Treatments Resistance in Cancer Management Abu, Michael Sunday; Yakubu, Ojochenemi Ejeh; Timothy, Mgbede; Umaru, Isaac John
African Multidisciplinary Journal of Sciences and Artificial Intelligence Vol 1 No 1 (2024): African Multidisciplinary Journal of Sciences and Artificial Intelligence
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/amjsai.v1i1.3739

Abstract

Cancer is a leading cause of death worldwide. Surgery is the primary treatment approach for cancer, but the survival rate is very low due to the rapid progression of the disease and presence of local and distant metastasis at diagnosis. Adjuvant chemotherapy and radiotherapy are important components of the multidisciplinary approaches for cancer treatment. However, resistance to radiotherapy and chemotherapy may result in treatment failure or even cancer recurrence. Radioresistance in cancer is often caused by the repair response to radiation-induced DNA damage, cell cycle dysregulation, cancer stem cells (CSCs) resilience, and epithelial-mesenchymal transition (EMT). Understanding the molecular alterations that lead to radioresistance may provide new diagnostic markers and therapeutic targets to improve radiotherapy efficacy. Patients who develop resistance to chemotherapy drugs cannot benefit from the cytotoxicity induced by the prescribed drug and will likely have a poor outcome with these treatments. Chemotherapy often shows a low response rate due to various drug resistance mechanisms. This review focuses on the molecular mechanisms of radioresistance and chemoresistance in cancer and discusses recent developments in therapeutic strategies targeting chemoradiotherapy resistance to improve treatment outcomes.
Molecular Basis of Chemotherapy and Radiotherapy Treatments Resistance in Cancer Management Abu, Michael Sunday; Yakubu, Ojochenemi Ejeh; Timothy, Mgbede; Umaru, Isaac John
African Multidisciplinary Journal of Sciences and Artificial Intelligence Vol 1 No 1 (2024): African Multidisciplinary Journal of Sciences and Artificial Intelligence
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/amjsai.v1i1.3739

Abstract

Cancer is a leading cause of death worldwide. Surgery is the primary treatment approach for cancer, but the survival rate is very low due to the rapid progression of the disease and presence of local and distant metastasis at diagnosis. Adjuvant chemotherapy and radiotherapy are important components of the multidisciplinary approaches for cancer treatment. However, resistance to radiotherapy and chemotherapy may result in treatment failure or even cancer recurrence. Radioresistance in cancer is often caused by the repair response to radiation-induced DNA damage, cell cycle dysregulation, cancer stem cells (CSCs) resilience, and epithelial-mesenchymal transition (EMT). Understanding the molecular alterations that lead to radioresistance may provide new diagnostic markers and therapeutic targets to improve radiotherapy efficacy. Patients who develop resistance to chemotherapy drugs cannot benefit from the cytotoxicity induced by the prescribed drug and will likely have a poor outcome with these treatments. Chemotherapy often shows a low response rate due to various drug resistance mechanisms. This review focuses on the molecular mechanisms of radioresistance and chemoresistance in cancer and discusses recent developments in therapeutic strategies targeting chemoradiotherapy resistance to improve treatment outcomes.
Production and Characterisation of Peroxidase from Aspergillus terrens Isolated from Water Sample in Wukari, Taraba State, Nigeria Timothy, Mgbede; Abah, Moses Adondua; Ayo, Victoria Ifeoluwa; Ahmed, Maryam Usman; Oyibo, Okpanachi Nuhu; Ijeomah, Nkechi Gift; Gbadebo, Adetoyi Moses; A, Ajala-Lawal R.; Najeeb, Aliyu Olamilekan; Morolake, Olabanji Martina; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel; Umaru, Isaac John
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3667

Abstract

The use of biocatalysts like enzymes in the development of environmentally sustainable processes has been reported as an ecofriendly approach for the current bioeconomy. This research aimed at producing and characterizing peroxidase from Aspergillus terrens isolated from water sample in Wukari, Taraba State, Nigeria. Fungi species from surface water sample obtained in Federal University Wukari, Taraba Nigeria were isolated through serial dilution of peptone water and was cultured in a room temperature in different petri dishes using Potato Dextrose Agar (PDA). A portion of the fungi growth chosen from the best growth of cultured water sample were cut and transferred into the 250 Erlenmeyer flask each containing an acceptable proportion of phosphate buffer, pH 6.0. The screening for peroxidase production was aseptically performed on a plate by inoculating the fermented medium with azur B dye. The appearance of a clear zone indicated a positive result for peroxidase activity. The incubation of the peroxidase enzyme was conducted over eleven days, with the highest enzyme yield observed on the eighth day. The results obtained in this study showed that the effect of pH on peroxidase activity was studied, revealing that the optimum pH for enzyme activity was 7.5. Peroxidase activity was lowest at pH 1. Temperature variation studies showed that the optimum temperature for peroxidase activity was 30°C. Peroxidase activity changed as substrate concentration was varied. Peroxidase activity was revealed to be highest at substrate concentration 1.9 mM. The kinetic parameters for peroxidase were investigated to be: Vmax = 10.57082; Km = 0.244186. The evidence presented in this research has shown that peroxidase can be produced in mass by using of white rot fungi from/around our surroundings and can be explored as a cheap source of peroxidase for industrial and biotechnological purposes. In conclusion, producing peroxidase from local isolates presents an eco-friendly and affordable alternative for industrial applications, contributing to environmental sustainability and public health.
Comparative Study on the Phytochemical and Micronutrients Levels in Selected Edible Mushroom Samples Abah, Moses Adondua; Adebisi, Arowora Kayode; Moses, Iyekekpolor Osamudiame; Dooshima, Arowora Mercy; Umaru, Isaac John; Uchechukwu, Ukoh Daniel; Timothy, Mgbede
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3707

Abstract

Edible mushrooms have been known to possess various phytochemical and micro nutrient levels. Edible mushrooms can be eaten and they supply nutritional benefits to the human body system. It is not widely consumed mainly due to paucity of information about it. Therefore, this research was conducted to compare the phytochemicals and micronutrients levels in two selected edible mushrooms (Pleurotus ostreatus and Agrocybe aegerita). Fully matured mushroom species of Oyster and Tea tree mushroom were collected, air dried and then stored in transparent polythene bags. Quantitative determination of phytochemicals and micronutrients (minerals and vitamins) present was carried out. Tea tree mushroom used in this research can be said to have the higher concentration of phytochemicals (phenols and poly-phenols), as there was no significant difference (p>0.05) in Oyster mushroom in comparison to Tea tree mushroom for phenols and poly-phenols. The results obtained from this research indicated that there was no significant decrease(p>0.05) in Oyster mushroom in comparison to Tea tree mushroom for Sodium, Iron, Magnesium, Selenium, Manganese. While there was a non-significant increase (p>0.05) in Oyster mushroom in comparison to Tea tree mushroom for Potassium, Calcium, Phosphorus, Copper. Tea tree mushroom had higher composition of vitamins A and C than oyster mushroom which were not significantly different (p>0.05). The findings in this study revealed that Tea tree mushroom (Agrocybe aegerita) is slightly better than Oyster mushroom (Pleurotus ostreatus) based on their phytochemicals and micronutrients (minerals and vitamins).
Extraction and Partial Purification of Protease from Fermented Beans Using Aspergillus niger Timothy, Mgbede; Emochone, Roy Yohanna; Umaru, Isaac John
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3728

Abstract

Proteases are enzymes that are found everywhere and play important roles in both the creation and breakdown of substances in living organisms. The utilization of microbial proteases in food fermentation is a long-standing practice that has now expanded to various industries due to advancements in the 'omics' era and genetic and protein engineering techniques. The protease enzyme was derived from Aspergillus niger, which was isolated from fermented cooked discarded beans. The enzyme was partially purified and described utilizing an affordable and environmentally friendly modified technique. The impact of temperature, pH, and manufacturing time on the enzyme was assessed. The optimal conditions for protease generation were a temperature of 40°C, a pH of 7.0, and duration of 6 days. The kinetic parameter values for Km and Vmax were determined to be 0.9181 mg/mL and 1.08914 µmol/min respectively. The protein determination experiment showed that the highest protein production occurred on day 6, following 5 days of optimal protein production. The research has shown that Aspergillus niger can efficiently generate protease at a low cost, making it suitable for various industrial applications including pharmaceuticals, food production, detergents, and cosmetics.
Production and Characterisation of Peroxidase from Aspergillus terrens Isolated from Water Sample in Wukari, Taraba State, Nigeria Timothy, Mgbede; Abah, Moses Adondua; Ayo, Victoria Ifeoluwa; Ahmed, Maryam Usman; Oyibo, Okpanachi Nuhu; Ijeomah, Nkechi Gift; Gbadebo, Adetoyi Moses; A, Ajala-Lawal R.; Najeeb, Aliyu Olamilekan; Morolake, Olabanji Martina; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel; Umaru, Isaac John
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3667

Abstract

The use of biocatalysts like enzymes in the development of environmentally sustainable processes has been reported as an ecofriendly approach for the current bioeconomy. This research aimed at producing and characterizing peroxidase from Aspergillus terrens isolated from water sample in Wukari, Taraba State, Nigeria. Fungi species from surface water sample obtained in Federal University Wukari, Taraba Nigeria were isolated through serial dilution of peptone water and was cultured in a room temperature in different petri dishes using Potato Dextrose Agar (PDA). A portion of the fungi growth chosen from the best growth of cultured water sample were cut and transferred into the 250 Erlenmeyer flask each containing an acceptable proportion of phosphate buffer, pH 6.0. The screening for peroxidase production was aseptically performed on a plate by inoculating the fermented medium with azur B dye. The appearance of a clear zone indicated a positive result for peroxidase activity. The incubation of the peroxidase enzyme was conducted over eleven days, with the highest enzyme yield observed on the eighth day. The results obtained in this study showed that the effect of pH on peroxidase activity was studied, revealing that the optimum pH for enzyme activity was 7.5. Peroxidase activity was lowest at pH 1. Temperature variation studies showed that the optimum temperature for peroxidase activity was 30°C. Peroxidase activity changed as substrate concentration was varied. Peroxidase activity was revealed to be highest at substrate concentration 1.9 mM. The kinetic parameters for peroxidase were investigated to be: Vmax = 10.57082; Km = 0.244186. The evidence presented in this research has shown that peroxidase can be produced in mass by using of white rot fungi from/around our surroundings and can be explored as a cheap source of peroxidase for industrial and biotechnological purposes. In conclusion, producing peroxidase from local isolates presents an eco-friendly and affordable alternative for industrial applications, contributing to environmental sustainability and public health.
Comparative Study on the Phytochemical and Micronutrients Levels in Selected Edible Mushroom Samples Abah, Moses Adondua; Adebisi, Arowora Kayode; Moses, Iyekekpolor Osamudiame; Dooshima, Arowora Mercy; Umaru, Isaac John; Uchechukwu, Ukoh Daniel; Timothy, Mgbede
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3707

Abstract

Edible mushrooms have been known to possess various phytochemical and micro nutrient levels. Edible mushrooms can be eaten and they supply nutritional benefits to the human body system. It is not widely consumed mainly due to paucity of information about it. Therefore, this research was conducted to compare the phytochemicals and micronutrients levels in two selected edible mushrooms (Pleurotus ostreatus and Agrocybe aegerita). Fully matured mushroom species of Oyster and Tea tree mushroom were collected, air dried and then stored in transparent polythene bags. Quantitative determination of phytochemicals and micronutrients (minerals and vitamins) present was carried out. Tea tree mushroom used in this research can be said to have the higher concentration of phytochemicals (phenols and poly-phenols), as there was no significant difference (p>0.05) in Oyster mushroom in comparison to Tea tree mushroom for phenols and poly-phenols. The results obtained from this research indicated that there was no significant decrease(p>0.05) in Oyster mushroom in comparison to Tea tree mushroom for Sodium, Iron, Magnesium, Selenium, Manganese. While there was a non-significant increase (p>0.05) in Oyster mushroom in comparison to Tea tree mushroom for Potassium, Calcium, Phosphorus, Copper. Tea tree mushroom had higher composition of vitamins A and C than oyster mushroom which were not significantly different (p>0.05). The findings in this study revealed that Tea tree mushroom (Agrocybe aegerita) is slightly better than Oyster mushroom (Pleurotus ostreatus) based on their phytochemicals and micronutrients (minerals and vitamins).
Extraction and Partial Purification of Protease from Fermented Beans Using Aspergillus niger Timothy, Mgbede; Emochone, Roy Yohanna; Umaru, Isaac John
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3728

Abstract

Proteases are enzymes that are found everywhere and play important roles in both the creation and breakdown of substances in living organisms. The utilization of microbial proteases in food fermentation is a long-standing practice that has now expanded to various industries due to advancements in the 'omics' era and genetic and protein engineering techniques. The protease enzyme was derived from Aspergillus niger, which was isolated from fermented cooked discarded beans. The enzyme was partially purified and described utilizing an affordable and environmentally friendly modified technique. The impact of temperature, pH, and manufacturing time on the enzyme was assessed. The optimal conditions for protease generation were a temperature of 40°C, a pH of 7.0, and duration of 6 days. The kinetic parameter values for Km and Vmax were determined to be 0.9181 mg/mL and 1.08914 µmol/min respectively. The protein determination experiment showed that the highest protein production occurred on day 6, following 5 days of optimal protein production. The research has shown that Aspergillus niger can efficiently generate protease at a low cost, making it suitable for various industrial applications including pharmaceuticals, food production, detergents, and cosmetics.