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All Journal BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains JSMARTech : Journal of Smart Bioprospecting and Technology
Kusumaningrum, Fransisca Victory
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Biochemistry, Genetics & Molecular Biology Chemistry Education Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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DNA Mini Barcoding untuk Autentikasi Produk Perikanan Kusumaningrum, Fransisca Victory; Purba, Laurentia Henrieta Permita Sari
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 8 No. 2 (2025): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v8i2.14329

Abstract

Fisheries production is predicted to continuously increase every year. However, the development of fishery products may result in ingredient substitution where high- value components are replaced with the lower-economic alternatives. Additionally, mislabelling cases also occur when the claims on packaging do not accurately reflect the actual ingredients contained in the product. The use of DNA mini barcoding using the cytochrome c oxidase subunit I (COI) gene has emerged as an innovative solution in species identification, particularly in fishery products with degraded DNA. This technique utilizes short DNA fragments from marker regions ranging from 100-200 bp. By employing shorter marker size, the amplification and species identification process achieve a success rate of approximately 90-95%. DNA mini barcoding offers several advantages, including higher efficiency, speed, and accuracy in identification process, while also being cost-effective. However, this technique has certain limitations such as challenges in distinguishing genetically similar species and the dependence on the quality of the DNA sample. Despite these constrains, DNA mini barcoding serves an alternative for the authentication process of processed food products that contributing to food safety and conservation efforts.   Keywords: Authentication; Cytochrome C Oxidase Subunit I; Mini-barcodes DNA; Fish Product
Phylogenetic study of Genus Scomberomorus based on cyt b gene in the north and south coast of Java, Indonesia Purba, Laurentia Henrieta Permita Sari; Sutan, Ditha Klarissa; Simanjuntak, Monayanti; Kusumaningrum, Fransisca Victory
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 6 No. 1 (2025): Vol. 6 No. 1 (2025): JSMARTech Volume 6, No 1, 2025
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2025.006.01.11

Abstract

Indonesia possessed megabiodiversity especially in marine animals. As the highly commercial commodities, the understanding for species distribution of Spanish mackerel and seerfish (Scomberomorus spp.) plays a key role for their sustainability. Thus, the aim of this study was to map the species distribution of Scomberomorus spp. in Java surrounding waters.  We collected 10 specimens from 4 landing sites in northern and southern coast of Java. Using partial cytochrome b gene fragment (~400 bp), we characterized the gene and haplotype diversity as well as the phylogenetic relationship. Three species of mackerel and seerfish were found in Java coastal line. The genetic differences Scomberomorus sp. ranged from 0 to 14%. By this study, we provide useful data that can be utilized as biodiversity monitoring. In addition, we confirmed that cythocrome b were effective for future application in fishery management and product authentication of mackerel in Java and surrounding areas.
Co-Authors Purba, Laurentia Henrieta Permita Sari Simanjuntak, Monayanti Sutan, Ditha Klarissa