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All Journal Bio-Lectura Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam JSMARTech : Journal of Smart Bioprospecting and Technology Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Purba, Laurentia Henrieta Permita Sari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics

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Phylogenetic study of Genus Scomberomorus based on cyt b gene in the north and south coast of Java, Indonesia Purba, Laurentia Henrieta Permita Sari; Sutan, Ditha Klarissa; Simanjuntak, Monayanti; Kusumaningrum, Fransisca Victory
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 6 No. 1 (2025): Vol. 6 No. 1 (2025): JSMARTech Volume 6, No 1, 2025
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2025.006.01.11

Abstract

Indonesia possessed megabiodiversity especially in marine animals. As the highly commercial commodities, the understanding for species distribution of Spanish mackerel and seerfish (Scomberomorus spp.) plays a key role for their sustainability. Thus, the aim of this study was to map the species distribution of Scomberomorus spp. in Java surrounding waters.  We collected 10 specimens from 4 landing sites in northern and southern coast of Java. Using partial cytochrome b gene fragment (~400 bp), we characterized the gene and haplotype diversity as well as the phylogenetic relationship. Three species of mackerel and seerfish were found in Java coastal line. The genetic differences Scomberomorus sp. ranged from 0 to 14%. By this study, we provide useful data that can be utilized as biodiversity monitoring. In addition, we confirmed that cythocrome b were effective for future application in fishery management and product authentication of mackerel in Java and surrounding areas.
EXPLORING THE FEEDING PATTERNS OF SIAMANGS: BEHAVIOR AND FOOD PREFERENCES IN ZOO ENVIRONMENTS AND THEIR CONTRIBUTION TO BIOLOGY EDUCATION Sisilia, Panjaitan Grace; Prihatmo, Guruh; Purba, Laurentia Henrieta Permita Sari
Bio-Lectura : Jurnal Pendidikan Biologi Vol. 12 No. 1 (2025)
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/bl.v12i1.25577

Abstract

The siamang (Symphalangus syndactylus), an endemic primate of Indonesia, is critically endangered due to hunting and habitat destruction and is now predominantly found in conservation areas. This study aims to analyze the feeding behavior and food preferences of siamangs in the zoo, evaluate the suitability of the ex-situ diet composition compared to their natural diet, and assess its contribution to biology education. The research methods included 480 hours of feeding behaviour observation using scan sampling, descriptive data analysis, and Pearson correlation tests. The results indicate that the siamang's diet in zoos is dominated by fruit (53.88% for males and 56.38% for females), followed by leaves and supplementary foods such as tubers and biscuits. The dietary composition is similar to studies conducted in their natural habitat, although the variety of zoo food is more limited. Adjustments to the feeding menu, such as hanging food at elevated locations and increasing the variety of local fruits, could support the siamangs' natural feeding behaviours. In conclusion, the feeding patterns of siamangs in zoos largely align with their natural behaviour as frugivores, although improvements in feeding methods and dietary variety are needed. The implications of this study emphasize the importance of aligning ex-situ conditions with natural habitats to support animal welfare and conservation programs while also strengthening the role of zoos as centres for education and biodiversity preservation.
A Mini Review of Short Tandem Repeat (STR) as DNA Profiling Markers in Forensic Biology Nitisara, Antonia Anindyanari Paramartastri; Purba, Laurentia Henrieta Permita Sari
Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam Vol. 22 No. 1 (2025): Sainmatika : Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/sainmatika.v22i1.17388

Abstract

The use of forensic biology methods plays an important role in handling criminal cases such as sexual violence, murder, and assault to enhance the quality of criminal investigation. These methods use polymorphic genetic markers such as short tandem repeat (STR) or microsatellites to identify individuals based on the DNA profile of the cell nucleus left on the evidence. STR markers are considered suitable for forensic analysis due to their ability to work with degraded DNA and the high heterozygosity, providing strong discrimination capabilities. The DNA profiling process involves collecting evidence samples, DNA extraction, PCR amplification, capillary electrophoresis, and comparison with national or international DNA databases. All processes follow standards to ensure accurate results. Challenges in STR analysis include the need for skilled analysts, protocol updates, database expansion, and ethical considerations regarding genetic data. Improvements in the forensic system will enhance law enforcement effectiveness and strengthen public trust in the future of forensic science.
Construction and optimization of human (Homo sapiens) mitochondrial DNA primers in the D-loop hypervariable segment II region in forensic biology Nitisara , Antonia Anindyanari Paramartastri; Saamia, Vira; Purba, Laurentia Henrieta Permita Sari
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1657

Abstract

DNA analysis is fundamental in forensic identification; however, nuclear DNA profiling often fails when samples are degraded. Mitochondrial DNA (mtDNA), which is maternally inherited and present in high copy numbers, provides a reliable alternative, particularly through analysis of the D-loop hypervariable segment II (HVS II). This study aimed to design and optimize human mtDNA HVS II–specific primers for forensic applications.An experimental approach combining in silico and in vitro methods was employed. Primer design and evaluation were conducted using the NCBI database, Primer3Plus, and NetPrimer Biosoft. Buccal swab samples from seven individuals were extracted using the PrepFiler™ Forensic DNA Extraction Kit, and DNA quality was assessed using a NanoVue spectrophotometer. Primer optimization was performed using gradient PCR, and sequencing was carried out using the Sanger method.Two primer pairs successfully amplified the HVS II region, generating fragments of 433–513 bp at an optimal annealing temperature of 56.5 °C. Sequence analysis revealed heteroplasmy and identified haplogroups N21 and L4b within the same maternal lineage. Both in silico and in vitro results confirmed that the designed primers specifically and reliably amplified human mtDNA.In conclusion, the optimized HVS II primers demonstrate strong potential for forensic casework involving degraded or limited biological samples and support improved resolution in population-level mtDNA analyses.
Co-Authors Kusumaningrum, Fransisca Victory Nitisara , Antonia Anindyanari Paramartastri Nitisara, Antonia Anindyanari Paramartastri Prihatmo, Guruh Saamia, Vira Simanjuntak, Monayanti Sisilia, Panjaitan Grace Sutan, Ditha Klarissa