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All Journal BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Bio-Lectura Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam JSMARTech : Journal of Smart Bioprospecting and Technology SERVIRISMA: Jurnal Pengabdian kepada Masyarakat
Purba, Laurentia Henrieta Permita Sari
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Other

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PELATIHAN KULTUR JARINGAN TUMBUHAN BAGI SISWA SMA NEGERI 7 YOGYAKARTA None, Ratih Restiani; Ariestanti, Catarina Aprilia; Purba, Laurentia Henrieta Permita Sari; Retnowati, Theresia Sri; Matheos, Josiah Herald; Kaban, Sarah Mega Pratenna; Sekar, Astrid Ayu
Servirisma Vol. 3 No. 2 (2023): Servirisma : Jurnal Pengabdian kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/servirisma.2023.32.44

Abstract

Plant tissue culture is one of the topics in the Biotechnology module of Biology for senior high school class XII. This topic has not been optimally provided in schools, especially in SMA Negeri 7 Yogyakarta due to the limited human resources who are experienced in plant tissue culture and the unavailability of facilities and equipment that support the implementation of the practicum topic. Therefore, this workshop aimed to help students gain knowledge and skills regarding the basic principles, benefits, and stages of plant tissue culture. The workshop was conducted in the Biology Laboratory of SMA Negeri 7 Yogyakarta and participated by 10 class XII students. The workshop consisted of questions and answers before the workshop, materials explanation, hands-on activities, questions and answers after the workshop, and evaluation. The material was delivered using lecturing methods and interactive discussions, followed by practical activities including preparation of culture media, sterilization and inoculation of explants and acclimatization which were carried out by engaging the active participation of each student. The results obtained in this workshop were that students were able to understand and implement plant tissue culture practical activities as well.  
PENGAJARAN DAN PRAKTIK UJI POTENSI PREBIOTIK PANGAN FUNGSIONAL LOKAL DI SMA NEGERI 7 YOGYAKARTA Ariestanti, Catarina; Nugroho, Wahyu Setyo; Kristiawan, Yoga Angkawijaya; Purba, Laurentia Henrieta Permita Sari; Restiani, Ratih
Servirisma Vol. 3 No. 2 (2023): Servirisma : Jurnal Pengabdian kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/servirisma.2023.32.59

Abstract

Application of Biotechnology in Indonesia has been found in many sectors especially in food sector. One of the benefits is that it can be used to increase the nutritional value of food product so that it can be beneficial for health and decrease the risk of disease. The introduction of Biotechnology in utilizing local food product will increase students’ sensitivity about natural materials and their utilization. The purpose of this program was to introduce the role of Biotechnology in utilizing and processing agriculture by-product into beneficial edible end-product. This activity was divided into three stages: preparation, implementation, and reporting the activity. Preparation was done by contacting 7 State Senior High School as a partner. Implementation was conducted in partner’s Laboratory of Biology by delivering the teaching materials, continued with producing banana corm cookies and checked its prebiotik capability. Banana corm cookies were successfully produced. In vitro study showed that Lactobacillus plantarum FNCC-0265 were able to grow in banana corm cookies substituted media. The students showed a positive response during the activity, and it was shown by creation of the cookies and their ability to use drop plate technique for bacterial enumeration in vitro.
DNA Mini Barcoding untuk Autentikasi Produk Perikanan Kusumaningrum, Fransisca Victory; Purba, Laurentia Henrieta Permita Sari
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 8 No. 2 (2025): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v8i2.14329

Abstract

Fisheries production is predicted to continuously increase every year. However, the development of fishery products may result in ingredient substitution where high- value components are replaced with the lower-economic alternatives. Additionally, mislabelling cases also occur when the claims on packaging do not accurately reflect the actual ingredients contained in the product. The use of DNA mini barcoding using the cytochrome c oxidase subunit I (COI) gene has emerged as an innovative solution in species identification, particularly in fishery products with degraded DNA. This technique utilizes short DNA fragments from marker regions ranging from 100-200 bp. By employing shorter marker size, the amplification and species identification process achieve a success rate of approximately 90-95%. DNA mini barcoding offers several advantages, including higher efficiency, speed, and accuracy in identification process, while also being cost-effective. However, this technique has certain limitations such as challenges in distinguishing genetically similar species and the dependence on the quality of the DNA sample. Despite these constrains, DNA mini barcoding serves an alternative for the authentication process of processed food products that contributing to food safety and conservation efforts.   Keywords: Authentication; Cytochrome C Oxidase Subunit I; Mini-barcodes DNA; Fish Product
Phylogenetic study of Genus Scomberomorus based on cyt b gene in the north and south coast of Java, Indonesia Purba, Laurentia Henrieta Permita Sari; Sutan, Ditha Klarissa; Simanjuntak, Monayanti; Kusumaningrum, Fransisca Victory
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 6 No. 1 (2025): Vol. 6 No. 1 (2025): JSMARTech Volume 6, No 1, 2025
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2025.006.01.11

Abstract

Indonesia possessed megabiodiversity especially in marine animals. As the highly commercial commodities, the understanding for species distribution of Spanish mackerel and seerfish (Scomberomorus spp.) plays a key role for their sustainability. Thus, the aim of this study was to map the species distribution of Scomberomorus spp. in Java surrounding waters.  We collected 10 specimens from 4 landing sites in northern and southern coast of Java. Using partial cytochrome b gene fragment (~400 bp), we characterized the gene and haplotype diversity as well as the phylogenetic relationship. Three species of mackerel and seerfish were found in Java coastal line. The genetic differences Scomberomorus sp. ranged from 0 to 14%. By this study, we provide useful data that can be utilized as biodiversity monitoring. In addition, we confirmed that cythocrome b were effective for future application in fishery management and product authentication of mackerel in Java and surrounding areas.
EXPLORING THE FEEDING PATTERNS OF SIAMANGS: BEHAVIOR AND FOOD PREFERENCES IN ZOO ENVIRONMENTS AND THEIR CONTRIBUTION TO BIOLOGY EDUCATION Sisilia, Panjaitan Grace; Prihatmo, Guruh; Purba, Laurentia Henrieta Permita Sari
Bio-Lectura : Jurnal Pendidikan Biologi Vol. 12 No. 1 (2025)
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/bl.v12i1.25577

Abstract

The siamang (Symphalangus syndactylus), an endemic primate of Indonesia, is critically endangered due to hunting and habitat destruction and is now predominantly found in conservation areas. This study aims to analyze the feeding behavior and food preferences of siamangs in the zoo, evaluate the suitability of the ex-situ diet composition compared to their natural diet, and assess its contribution to biology education. The research methods included 480 hours of feeding behaviour observation using scan sampling, descriptive data analysis, and Pearson correlation tests. The results indicate that the siamang's diet in zoos is dominated by fruit (53.88% for males and 56.38% for females), followed by leaves and supplementary foods such as tubers and biscuits. The dietary composition is similar to studies conducted in their natural habitat, although the variety of zoo food is more limited. Adjustments to the feeding menu, such as hanging food at elevated locations and increasing the variety of local fruits, could support the siamangs' natural feeding behaviours. In conclusion, the feeding patterns of siamangs in zoos largely align with their natural behaviour as frugivores, although improvements in feeding methods and dietary variety are needed. The implications of this study emphasize the importance of aligning ex-situ conditions with natural habitats to support animal welfare and conservation programs while also strengthening the role of zoos as centres for education and biodiversity preservation.
A Mini Review of Short Tandem Repeat (STR) as DNA Profiling Markers in Forensic Biology Nitisara, Antonia Anindyanari Paramartastri; Purba, Laurentia Henrieta Permita Sari
Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam Vol. 22 No. 1 (2025): Sainmatika : Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/sainmatika.v22i1.17388

Abstract

The use of forensic biology methods plays an important role in handling criminal cases such as sexual violence, murder, and assault to enhance the quality of criminal investigation. These methods use polymorphic genetic markers such as short tandem repeat (STR) or microsatellites to identify individuals based on the DNA profile of the cell nucleus left on the evidence. STR markers are considered suitable for forensic analysis due to their ability to work with degraded DNA and the high heterozygosity, providing strong discrimination capabilities. The DNA profiling process involves collecting evidence samples, DNA extraction, PCR amplification, capillary electrophoresis, and comparison with national or international DNA databases. All processes follow standards to ensure accurate results. Challenges in STR analysis include the need for skilled analysts, protocol updates, database expansion, and ethical considerations regarding genetic data. Improvements in the forensic system will enhance law enforcement effectiveness and strengthen public trust in the future of forensic science.
Co-Authors Ariestanti, Catarina Ariestanti, Catarina Aprilia Kaban, Sarah Mega Pratenna Kristiawan, Yoga Angkawijaya Kusumaningrum, Fransisca Victory Matheos, Josiah Herald Nitisara, Antonia Anindyanari Paramartastri None, Ratih Restiani Nugroho, Wahyu Setyo Prihatmo, Guruh Ratih Restiani Retnowati, Theresia Sri Sekar, Astrid Ayu Simanjuntak, Monayanti Sisilia, Panjaitan Grace Sutan, Ditha Klarissa