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All Journal Journal of Applied Food Technology JSMARTech : Journal of Smart Bioprospecting and Technology
Simanjuntak, Monayanti
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Other

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Effect of Fermentation Time on the Antioxidant and Antibacterial Activity of Fermented Cascara Beverage Simanjuntak, Monayanti; Hutabarat, Diva Yolanda; Sutan, Ditha Klarissa; Harjanto, Eunike; Aditiyarini, Dwi
Journal of Applied Food Technology Vol 11, No 2 (2024)
Publisher : Dept. Food Technology, Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jaft.24038

Abstract

Indonesia is a country with a high consumption of coffee, which is in direct comparison with the increased production of coffee waste. Arabica coffee husk (cascara) is coffee industry waste that has not been utilized optimally, which is only 0.76% of the existing potential. Cascara is known to have a secondary metabolite compound that can be utilized as a good product for human health. The effects of Arabica cascara fermentation time on the antioxidant and antibacterial activity of cascara beverage were studied in this study. This research is using a completely randomized non-factorial design. Cascara was obtained from Puntang Mountain, West Java, and was fermented with Lactiplantibacillus plantarum (previously known as Lactobacillus plantarum) for 10, 14, and 18 days. pH value was measured with a pH meter, total sugar content with phenol sulfuric acid method, caffeine content with high performance liquid chromatography (HPLC) method, total lactic acid bacteria (LAB) with drop plate method, total flavonoid and phenolic content with UV-Vis spectrophotometry method, antioxidant activity with 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and antibacterial activity against Helicobacter pylori with disc diffusion method. Data were analyzed using One-Way ANOVA and DMRT test at the significance level of 0.05. The results showed that prolonged fermentation times reduce the quality of Arabica cascara beverage. The optimal fermentation times were found to be 10 days producing the best balance of pH (3.66), total sugar content (1.29%), caffeine content (0.043%), total LAB (1 x 107), total phenols (12.74 ppm), total flavonoids (3.40 ppm) and highest antioxidant IC50 activity (215 ppm) while for antibacterial activity has not been able to inhibit the growth of Helicobacter pylori.
Phylogenetic study of Genus Scomberomorus based on cyt b gene in the north and south coast of Java, Indonesia Purba, Laurentia Henrieta Permita Sari; Sutan, Ditha Klarissa; Simanjuntak, Monayanti; Kusumaningrum, Fransisca Victory
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 6 No. 1 (2025): Vol. 6 No. 1 (2025): JSMARTech Volume 6, No 1, 2025
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2025.006.01.11

Abstract

Indonesia possessed megabiodiversity especially in marine animals. As the highly commercial commodities, the understanding for species distribution of Spanish mackerel and seerfish (Scomberomorus spp.) plays a key role for their sustainability. Thus, the aim of this study was to map the species distribution of Scomberomorus spp. in Java surrounding waters.  We collected 10 specimens from 4 landing sites in northern and southern coast of Java. Using partial cytochrome b gene fragment (~400 bp), we characterized the gene and haplotype diversity as well as the phylogenetic relationship. Three species of mackerel and seerfish were found in Java coastal line. The genetic differences Scomberomorus sp. ranged from 0 to 14%. By this study, we provide useful data that can be utilized as biodiversity monitoring. In addition, we confirmed that cythocrome b were effective for future application in fishery management and product authentication of mackerel in Java and surrounding areas.
Co-Authors Dwi Aditiyarini Harjanto, Eunike Hutabarat, Diva Yolanda Kusumaningrum, Fransisca Victory Purba, Laurentia Henrieta Permita Sari Sutan, Ditha Klarissa