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All Journal KOVALEN: Jurnal Riset Kimia Science and Technology Indonesia
Naser, Inda Hastuti N
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Materials Science & Nanotechnology Physics

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Decolorization of Synthetic Dyes by Laccase of Phellinus noxius BRB 11 Immobilized with Halloysite Nanotubes Naser, Inda Hastuti N; Kemala, Tetty; Yanto, Dede Heri Yuli; Purwaningsih, Henny; Nurhayat, Oktan Dwi; Ardiati, Fenny Clara; Bakar, N.H.H Abu; Iqbal, Anwar
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.644-659

Abstract

Halloysite nanotubes (HNTs) have been proposed as promising supports for enzyme immobilization. Developing an effective technique for enzyme immobilization on HNTs is crucial for their practical application. In this study, we present a method for immobilizing laccase enzymes on HNTs using various amino acids as green cross-linkers for eco-friendly decolorization of synthetic dye. The modified HNTs were characterized using FT-IR, FE-SEM, and HR-TEM analyses. Furthermore, the effects of various parameters—including pH (ranging from 3 to 8), temperature (30-70°C), toxicity, and reusability—on the activity of both free and immobilized laccase were investigated. The results indicated that glycine (Gly) provided the most effective support for laccase immobilization on HNTs. The modified HNTs/Gly-Lac achieved an immobilization yield of 98.45% and successfully decolorized 94.60% of Acid Blue 129 (AB129) within 2 h. The successful immobilization of laccase onto functionalized HNTs was confirmed through FT-IR, FE-SEM, and HR-TEM analyses. Morphological observations of HNTs/Gly for laccase immobilization showed a thickened and roughened appearance with a thin, light gray layer on the outer surface. Additionally, the presence of an imine (-C=N-) bond peak and a peak at 1410 cm-1, corresponding to the (-NH) bond, suggested successful covalent immobilization. The toxicity analysis revealed a reduction in the toxicity of laccase after immobilization onto modified HNTs. A reusability assessment conducted over five cycles demonstrated that decolorization efficiency remained above 45%. These findings suggest that HNTs/GlyLac could serve as an effective material for textile dye wastewater treatment.
Penentuan Kadar Albumin Ikan Gabus (Channa striata) dengan Metode Biuret setelah Dikukus dan Dipepes: Determination of Albumin Content of Snakehead Fish (Channa striata) by Biuret Method after Steamed and Wrapped Naser, Inda Hastuti N; Bahri, Syaiful; Satrimafitrah, Pasjan; Razak, Abd. Rahman; Ruslan; Ridhay, Ahmad; Puspitasari, Dwi Juli; Khairuddin
KOVALEN: Jurnal Riset Kimia Vol. 9 No. 2 (2023): August Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2023.v9.i2.16391

Abstract

Snakehead fish (Channa striata) is a type of freshwater fish that contains high levels of protein albumin. The research was conducted to determine the albumin content in snakehead fish after the cooking process, both steamed and wrapped. The research was conducted using a completely randomized design with a factorial pattern, the first factor was the cooking method (steamed and wrapped) and the second was cooking time (10, 15, 20, and 20 minutes). Determination of protein albumin levels in snakehead fish after processing using the Biuret method. The steaming process for 15 minutes resulted in protein content in the snakehead fish of 0.76%, while the protein albumin that was lost was 19.28%. The wrapped method for 20 minutes has a remaining protein content of 0.59%, while the missing protein is 38.78%. The method of processing snakehead fish by steaming is better than the wrapping process.
Co-Authors Abd. Rahman Razak Ahmad Ridhay Ardiati, Fenny Clara Bakar, N.H.H Abu Dede Heri Yuli YANTO Dwi Juli Puspitasari Henny Purwaningsih Iqbal, Anwar Khairuddin Nurhayat, Oktan Dwi Pasjan Satrimafitrah Ruslan SYAIFUL BAHRI Tetty Kemala