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All Journal Jurnal Pembelajaran dan Biologi Nukleus Bioscientist : Jurnal Ilmiah Biologi
Lamante, Nazwa S.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences

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Explorating Flower Morphology and Pollen Types of Various Angiospermae Families from Huluduotamo Village, Gorontalo Thalib, Moh. Randy Putra Pratama; Lamante, Nazwa S.; Tandililing, Indayani; Ibrahim, Rawiyanti; Mahluk, Linda K.; Ishak, Sri Layla Putri; Mantali, Magfirah F.; Djafar, Nazwa H.; Bano, Nurhayati S.; Ahmad, Jusna; Febriyanti, Febriyanti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6792

Abstract

Huluduotamo village is one of the villages in Suwawa District, Bone Bolango Regency, Gorontalo Province. The village is situated in the middle of the countryside within the Suwawa district area. The potential of Huluduotamo village is the potential of natural resources; thus, the exploration of flower morphology and pollen types of various angiosperm families was carried out in the village. Therefore, this study aims to look at and figure out the shape of pollen from different plant families, mainly Malvaceae and Fabaceae, by looking at pollen units, aperture types, wall thickening, and exine decoration. We conducted the sampling in the Huluduotamo village, Gorontalo, using direct observation methods and descriptive analysis under a microscope. The study found that the pollen from the Hibiscus rosa-sinensis L. plant in the malvaceae family has a monad pollen unit, an apantoporate aperture type, thickening of the centrifugal wall, and decoration on the echinate exine. Some plants, like Caesalpinia pulcherrima L. in the Fabaceae family, have pollen that is made up of a single unit, an atricolporate aperture for the red variety and a parasyncolporate aperture for the yellow variety, and psilate axine decoration. The similarity of the monad pollen unit shows how angiosperm plants reproduce, while differences in aperture types and axine ornamentation show how the shape of the plant has changed to support reproduction. This research provides important information for the identification of plant species and supports flowering plants
Identifikasi Tahap Perkembangan Mikrospora melalui Ukuran Floret pada Jantung Pisang Tanduk Pagalla, Devi Bunga; Ahmad, Jusna; Husain, Indriati; Lamante, Nazwa S.; Eyato, Rika
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.17681

Abstract

This study aimed to identify the relationship between floret length and the developmental stages of microspores in banana male inflorescence (Musa spp. cv. Tanduk), and to determine the optimal floret size range as an explant source for initiating microspore culture. Ten bract layers were analyzed, with five florets randomly selected from each layer and measured for their length. Anthers were extracted from each floret, crushed in sterile water, and observed microscopically using a wet mount preparation. The results revealed a strong correlation between floret length and microspore developmental stages. Microspores at the late uninucleate (vacuolated) to early bicellular stages were consistently found in florets ranging from 4.68 to 4.34 cm in length. Based on these findings, florets within the 4.60 to 4.00 cm range are recommended as the optimal size for microspore culture initiation, as they contain a higher proportion of developmentally competent microspores for embryogenesis induction. In conclusion, this study provides essential morphological and cytological criteria to support efficient explant selection for in vitro regeneration of banana through the microspore culture approach.
Co-Authors Bano, Nurhayati S. Devi Bunga Pagalla Djafar, Nazwa H. Eyato, Rika febriyanti febriyanti Ibrahim, Rawiyanti Indriati Husain Ishak, Sri Layla Putri Jusna Ahmad Mahluk, Linda K. Mantali, Magfirah F. Tandililing, Indayani Thalib, Moh. Randy Putra Pratama