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Chitin extraction from house crickets (acheta domesticus) using a green method with sonication heating Shakila, Tasya Nur; Sonia, Gita; Anjani, Cindy Salsabilla; Pambudi, Teguh; Umam, Hilman Imadul; Fauzie, Azis Kemal; Putri, Fajar Amelia Rachmawati
Sainteks: Jurnal Sain dan Teknik Vol 7 No 01 (2025): Maret
Publisher : Universitas Insan Cendekia Mandiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37577/sainteks.v7i01.867

Abstract

Chitin extraction from house crickets (Acheta domesticus) has been optimized by combining a green method using Deep Eutectic Solvent (DES) and sonication heating, thereby reducing environmental impact and improving time efficiency. The extraction process consisted of insect preparation, delipidation, demineralization, and deproteinization. The results show that the green process method with DES and sonication heating is effective in enhancing time efficiency, reducing mineral content by 56.52%, decreasing protein content by 84.94%, and increasing the degree of acetylation by 21%. However, there was a slight difference in the deproteinization results when compared to the cricket samples treated with reflux. FTIR analysis confirmed the purity and structural characteristics of the extracted chitin
Pengaruh jenis asam organik pada ekstraksi kitin dari jangkrik rumah (Acheta domesticus) melalui metode Green Process Sonia, Gita; Umam, Hilman Imadul; Pambudi, Teguh; Fauzie, Azis Kemal; Putri, Fajar Amelia Rachmawati; Shakila, Tasya Nur; Anjani, Cindy Salsabilla
Jurnal Penelitian Sains Vol 27, No 2 (2025)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56064/jps.v27i2.1152

Abstract

Penelitian saat ini bertujuan untuk mengembangkan kitin yang ramah lingkungan dan ekonomis dengan berbagai macam aplikasi menggunakan asam organik untuk demineralisasi jangkrik rumah. Sampel kitin diekstraksi dari jangkrik rumah (Acheta domesticus) dan langkah demineralisasi dilakukan dengan tiga asam organik (sitrat, asetat, dan oksalat). Kitin yang diekstraksi dengan asam asetat pada proses demineralisasi menunjukkan hasil ekstraksi tertinggi dengan hasil sebesar 6% dibandingkan asam organik lainnya. Sedangkan pada proses deproteinasi menggunakan larutan DES (Deep Eutectic Solvent) sampel yang diesktraksi menggunakan asam oksalat berhasil mendapatkan %DP sebesar 97,7% yang artinya hampir seluruh kandungan protein pada sampel berkurang. Pita karakteristik dan gugus fungsi kitin diidentifikasi dengan spektrum FTIR yang menunjukan struktur kitin pada penelitian ini sama dengan kitin komersil. Pada penelitian ini, asam organik sama efektifnya dengan asam mineral untuk demineralisasi jangkrik rumah. Proses yang dikembangkan membuka kemungkinan untuk memproduksi kitin dengan cara yang lebih ramah lingkungan dan dengan biaya yang lebih rendah di banyak sektor industri.
PEMANFAATAN BELALANG SEBAGAI SUMBER KITIN MELALUI PROSES EKSTRAKSI RAMAH LINGKUNGAN Anjani, Cindy Salsabilla; Umam, Hilman Imadul; Pambudi, Teguh; Fauzie, Azis Kemal; Putri, Fajar Amelia Rachmawati
Jurnal Al Ulum LPPM Universitas Al Washliyah Medan Vol. 13 No. 2 (2025): Jurnal Al Ulum: LPPM Universitas Al Washliyah Medan
Publisher : UNIVERSITAS AL WASHLIYAH (UNIVA) MEDAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47662/alulum.v13i2.912

Abstract

This study aimed to extract chitin from rice grasshoppers using the Deep Eutectic Solvent (DES) method. The chitin extraction process involves several stages, namely delipidation, demineralization, and deproteination. In the demineralization and deproteination stages, two extraction methods were compared, namely the conventional method using strong acids and bases and the environmentally friendly method using Deep Eutectic Solvent (DES). The conventional method uses hydrochloric acid (HCl) for demineralization and sodium hydroxide (NaOH) for deproteination. DES is an effective solvent in dissolving proteins without damaging the chitin structure. This success was evidenced by test parameters of fat content from 30.91 to 9.01 with a DL percentage of 70.85%. In the ash content test, it was proven that the % ash content in pure grasshoppers was 4.87% and decreased in the demineralization process to 1.38% for the extraction stage using DES from 1.25 in the extraction process using the conventional method, this shows that the demineralization extraction process was successful in reducing mineral levels. In the protein content test, pure grasshoppers were 53.57% decreased in the deproteination process using DES to 1.11% and in the conventional method using NaOH, it decreased by 1.65%, this proves a decrease in protein content in the deproteination stage. In the chitin extraction process, an infrared spectrum analysis (FTIR) was also carried out on the extracted chitin samples.