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All Journal Biology, Medicine, & Natural Product Chemistry Abdimas Galuh : Jurnal Pengabdian Kepada Masyarakat
Yanti, Novirma
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health

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Edukasi Pembuatan Pakan Silase Ruminansia dengan Kultur Campuran Lactobacillus sp., Rhodopseudomonas sp., Actinomycetes sp., Streptomyces sp., dan Yeast Savitri, Lisa; Prasetyawan, Fendy; Saristiana, Yuneka; Juwita, Syntia Tanu; Yanti, Novirma; Edda, Ilda Rambu
Abdimas Galuh Vol 7, No 1 (2025): Maret 2025
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v7i1.18152

Abstract

Pada musim kemarau, ketersediaan tumbuhan alami seperti rumput dan kacang-kacangan menurun, menyebabkan kesulitan bagi peternak dalam memenuhi kebutuhan pakan ternak ruminansia. Hal ini dapat berdampak negatif pada kesehatan dan produktivitas hewan ternak. Pakan ternak ruminansia terdiri dari berbagai jenis makanan, terutama tumbuhan, seperti rumput, kacang-kacangan, dan tanaman lain yang kaya serat dan nutrisi. Tumbuhan ini merupakan sumber utama energi bagi hewan ternak ruminansia seperti sapi, domba, dan kambing. Namun, selama musim kemarau, kekurangan tumbuhan menjadi tantangan utama. Salah satu solusi yang diusulkan adalah pembuatan silase. Silase adalah jenis pakan yang dihasilkan dari proses ensilase, di mana tumbuhan seperti rumput diawetkan dengan mengurangi kadar oksigen dan meningkatkan kadar asam laktat. Proses ini membantu mengawetkan nutrisi dalam tumbuhan dan menyediakan pakan tambahan untuk ternak saat musim kemarau. Program pengabdian masyarakat ini bertujuan untuk memberikan edukasi kepada peternak mengenai teknik pembuatan silase ruminansia menggunakan kultur campuran bakteri tertentu. Diharapkan program ini dapat meningkatkan pengetahuan dan keterampilan peternak dalam memproduksi pakan silase untuk memenuhi kebutuhan ternak ruminansia selama musim kemarau. Universitas Kadiri melalui program Pengabdian Masyarakat ini dapat ikut berkontribusi dalam upaya untuk mendukung program pemerintah yaitu “Making Indonesia 4.0” dalam peningkatan kualitas sumber daya manusia (SDM) dan pembangunan ekosistem inovasi. Melalui publikasi ilmiah, panduan, dan peningkatan pemahaman masyarakat, program ini diharapkan dapat memberikan dampak positif bagi peternak dan ekonomi lokal.
In Vitro Evaluation of the Antibacterial Properties of Lime Mistletoe (Dendrophthoe petandra (L.) Miq.) Extract Against Escherichia coli Savitri, Lisa; Mahardika, Juan Vega; Yanti, Novirma; Juwita, Syntia Tanu
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.641-644

Abstract

The increasing prevalence of antibiotic-resistant bacteria necessitates the exploration of alternative antimicrobial agents derived from natural sources. This study aimed to evaluate the in vitro antibacterial activity of lime mistletoe (Dendrophthoe petandra (L.) Miq.) extract against Escherichia coli ATCC 25922 using the disc diffusion method. Extract concentrations of 20%, 40%, 60%, 80%, and 100% were prepared alongside negative (distilled water) and positive (Cefadroxil) controls. Nutrient Agar (NA) media were inoculated with E. coli and discs impregnated with each concentration were placed on the media, followed by incubation at 37°C for 24 hours. The inhibition zones were measured to assess antibacterial activity. Results demonstrated a concentration-dependent increase in antibacterial efficacy, with inhibition zone diameters ranging from 8.3 mm at 20% extract to 20.3 mm at 100% extract concentration. The highest concentration’s inhibition zone was comparable to that of Cefadroxil (22.5 mm). No inhibition was observed in the negative control. Statistical analysis confirmed significant differences between treatment groups (p < 0.05). The antibacterial activity of lime mistletoe extract is likely due to its bioactive phytochemicals such as flavonoids and phenolics, which are known to disrupt bacterial cell membranes and inhibit microbial growth. These findings suggest that Dendrophthoe petandra extract holds potential as a natural antibacterial agent, providing an alternative to conventional antibiotics amid growing resistance issues. Further studies involving isolation of active compounds, toxicity evaluation, and in vivo testing are recommended to validate its clinical applicability.
Isolation and Identification of Dermatophytes from Toenails and Interdigital Spaces of Students Using Czapek Yeast Extract Agar Savitri, Lisa; Ihsan, Kharisul; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo; Yanti, Novirma; Hilmi, Mochamad Hanif
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.795-798

Abstract

Dermatophytosis remains a common superficial fungal infection affecting nails and interdigital spaces, with Trichophyton rubrum and T. mentagrophytes as the predominant etiologic agents. The recent emergence of terbinafine-resistant T. indotineae has raised new challenges in diagnosis and treatment. Although Sabouraud Dextrose Agar is the conventional medium for fungal culture, alternative media such as Czapek Yeast Extract Agar (CYA) may provide additional advantages for dermatophyte isolation and morphological identification. This study aimed to isolate and identify dermatophytes from toenails and interdigital spaces of university students using CYA medium and to evaluate its applicability in dermatophyte culture. A cross-sectional descriptive study was conducted among 100 university students aged 18–24 years. Specimens were collected from toenails (n = 60) and interdigital spaces (n = 40). Direct microscopy was performed using 20% KOH preparation. Samples were cultured on CYA supplemented with chloramphenicol and cycloheximide, and incubated at 28 ± 2 °C for up to 21 days. Fungal isolates were identified based on macroscopic and microscopic morphology. Data were analyzed descriptively, and associations were tested using the chi-square test. Fungal elements were detected in 65% of samples by KOH examination, and dermatophyte growth was confirmed in 52% of cultures. The most frequently isolated species were T. rubrum (28%) and T. mentagrophytes (18%). Notably, T. indotineae was detected in 5% of samples. No significant difference was found between toenail and interdigital isolates (p = 0.26). Dermatophytes are prevalent among university students, with T. rubrum as the dominant species. The detection of T. indotineae highlights its emerging role in young populations. CYA proved effective for dermatophyte isolation and may serve as an alternative culture medium in academic and diagnostic laboratories.
Co-Authors Edda, Ilda Rambu Elfred Rinaldo Kasimo, Elfred Rinaldo Fendy Prasetyawan Hilmi, Mochamad Hanif Ihsan, Kharisul Juwita, Syntia Tanu Mahardika, Juan Vega Rochmad Krissanjaya savitri, Lisa Yuneka Saristiana