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All Journal Biosaintifika: Journal of Biology & Biology Education
Pa’ee, Furzani
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Agriculture, Biological Sciences & Forestry

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Effect of BAP and Picloram on Shoot Induction (Musa acuminata Colla var. Mulu Bebek) Arief, Nurul Huda Am Zen; Rahayu, Enni Suwarsi; Pa’ee, Furzani; Habibah, Noor Aini
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 1 (2025): April 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i1.19605

Abstract

Musa acuminata Colla var. Mulu Bebek is a high-quality banana from Ternate, North Maluku. Its conventional cultivation faces challenges due to Fusarium wilt, reducing productivity. In vitro culture offers a solution for rapid, disease-free seedling production. This study aims to examine the effect of BAP and picloram on shoot induction using a CRFD at various concentrations (0–3 ppm) and evaluate the percentage of live explants, emergence time, and number and length of shoots and roots. Results show that 1 ppm picloram accelerates root emergence, while 0 ppm promotes the fastest shoot emergence, the longest shoot and root lengths. 1 ppm BAP increases shoot numbers. The interaction of picloram and BAP has a significant effect on the parameters of the fastest root emergence time, namely 1 ppm picloram + 0 ppm BAP, 1 ppm and 3 ppm, the fastest shoot emergence time, namely without the addition of PGR, and the longest shoot length, 0 ppm picloram + 2 ppm BAP. The combination of 0 ppm picloram + 1 ppm BAP is the optimal condition for a percentage of 100% live explants, the fastest time for root emergence, the highest number of shoots and roots, the longest shoot and root length and the optimal condition for the fastest time for shoot emergence is 0 picloram + 0 ppm BAP. This study provides insight into the role of BAP and picloram in inducing banana shoots that has not been reported before. The micropropagation media formula obtained in this study is an effort to improve the quality of healthy banana seedlings in the future.
Enhancing Plantlet Growth of Vanda floresensis Motes through Acclimatization Chamber, Chitosan Spraying, and Mixed Medium Rahayu, Enni Suwarsi; Habibah, Noor Aini; Anggraito, Yustinus Ulung; Melati, Dinda; Sinaga, Na Dame Maulibulung; Pa’ee, Furzani
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.33383

Abstract

The conservation of  Vanda floresensis Motes has been conducted using in vitro techniques and has produced a large number of plantlets. These plantlets require acclimatization, but the optimal method has not yet been found. The aims of the research were to develop an acclimatization protocol by verifying the effectiveness of an acclimatization chamber (AC), chitosan spraying, and mixed medium composition on plantlet growth.  The research was conducted in a screen house of Universitas Negeri Semarang, using a factorial randomized block design with three factors. The AC factor consisted of two levels: inside and outside the AC. The chitosan concentration factor consisted of five levels, namely 0.0, 1.5, 3.0, 4.5, and 6.0 ppm. The composition of the mixed medium consisted of three levels. The observed variables included the increase in number, length, and width of the leaf, total chlorophyll content, and wilted leaf. Data were analyzed by ANOVA and Duncan test. It can be concluded that the use of AC, chitosan spraying, and mixed medium composition was effective in increasing leaf growth and total chlorophyll content and reducing wilted leaf.  Spraying chitosan of 1.5 - 3.0 ppm with the use of AC and spraying chitosan of 1.5 ppm with a mixed medium of brick pieces, coconut fibre, and tree bark chips with a ratio of 1:2:1 resulted in the highest leaf growth and total chlorophyll content, and the lowest wilted leaf. The results of this study can be used as an acclimatization protocol for orchid propagation for conservation purposes.
Co-Authors Arief, Nurul Huda Am Zen Enni Suwarsi Rahayu Melati, Dinda Noor Aini Habibah Sinaga, Na Dame Maulibulung Yustinus Ulung Anggraito