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All Journal Biosaintifika: Journal of Biology & Biology Education
Sinaga, Na Dame Maulibulung
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Agriculture, Biological Sciences & Forestry

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Enhancing Plantlet Growth of Vanda floresensis Motes through Acclimatization Chamber, Chitosan Spraying, and Mixed Medium Rahayu, Enni Suwarsi; Habibah, Noor Aini; Anggraito, Yustinus Ulung; Melati, Dinda; Sinaga, Na Dame Maulibulung; Pa’ee, Furzani
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.33383

Abstract

The conservation of  Vanda floresensis Motes has been conducted using in vitro techniques and has produced a large number of plantlets. These plantlets require acclimatization, but the optimal method has not yet been found. The aims of the research were to develop an acclimatization protocol by verifying the effectiveness of an acclimatization chamber (AC), chitosan spraying, and mixed medium composition on plantlet growth.  The research was conducted in a screen house of Universitas Negeri Semarang, using a factorial randomized block design with three factors. The AC factor consisted of two levels: inside and outside the AC. The chitosan concentration factor consisted of five levels, namely 0.0, 1.5, 3.0, 4.5, and 6.0 ppm. The composition of the mixed medium consisted of three levels. The observed variables included the increase in number, length, and width of the leaf, total chlorophyll content, and wilted leaf. Data were analyzed by ANOVA and Duncan test. It can be concluded that the use of AC, chitosan spraying, and mixed medium composition was effective in increasing leaf growth and total chlorophyll content and reducing wilted leaf.  Spraying chitosan of 1.5 - 3.0 ppm with the use of AC and spraying chitosan of 1.5 ppm with a mixed medium of brick pieces, coconut fibre, and tree bark chips with a ratio of 1:2:1 resulted in the highest leaf growth and total chlorophyll content, and the lowest wilted leaf. The results of this study can be used as an acclimatization protocol for orchid propagation for conservation purposes.
The Environmental Factors Enhance the Growth and Survival of Rhizophora Seedlings Sinaga, Na Dame Maulibulung; Pradipta, Aruna; Martuti, Nana Kariada Tri; Jabar, Muhammad Abdul; Soleha, Iro Datus
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.35777

Abstract

Coastal ecosystems are synonymous with mangrove trees. Threatened mangrove existence is tantamount to reduced biological existence, weakened coastal protection, and reduced carbon storage. Moreover, mangrove growth naturally depends on the quality of seedlings supported by proper nursery management. This study aims to evaluate the impact of four environmental factors (light intensity, salinity, pH, and tidal frequency) on the growth and survival of Rhizophora: Rhizophora mucronata, Rhizophora stylosa, and Rhizophora apiculata cultivated in nurseries with a water system integrated with the Internet of Things (IoT). A single-factor design was applied, with each treatment bed containing 600 seedlings (200 per species) and an uncontrolled bed as a control. Weekly random sampling of 20% of seedlings from each treatment for eight weeks. Data were analyzed using a two-way ANOVA with treatment and species as fixed factors, followed by the Tukey HSD test at a significance level of 5%. The results showed that R. mucronata in the light treatment (without shade net) achieved the highest survival rate (98.8%) and the largest stem diameter, which was 0.698 cm. In addition, the highest seedling height was R. mucronata in the high-yield salinity treatment, reaching 56.78 cm, and the highest number of leaves was recorded in R. apiculata in the salinity treatment, with an average of 6.92 leaves per seedling. IoT-integrated nurseries stabilize water supply and tidal cycles, reduce mortality to below 10%, and enhance seedling quality. These findings indicate that nursery environmental management can improve the consistency of mangrove seedling growth and support large-scale restoration.
Co-Authors Enni Suwarsi Rahayu Jabar, Muhammad Abdul Melati, Dinda Nana Kariada Trimartuti Noor Aini Habibah Pa’ee, Furzani Pradipta, Aruna Soleha, Iro Datus Yustinus Ulung Anggraito