Vebryansyah, Alhady Putra
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EFFECTIVENESS OF AJWA DATE SEED EXTRACT EFFERVESCENT TABLETS (Phoenix dactylifera L.) AGAINST MICROORGANISMS ON ACRYLIC RESIN Amiruddin, Maqhfirah; Pertiwisari, Amanah; Vebryansyah, Alhady Putra; Nurasisa Lestari, Nurasisa; Sembiring, Ira Asnita
Dentino: Jurnal Kedokteran Gigi Vol 10, No 1 (2025)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v10i1.22210

Abstract

Background: Suboptimal denture cleaning methods present a problem for denture users, leading to the buildup of bacteria and fungi on the denture base. Streptococcus mutans is a bacterium involved in plaque formation, while Candida albicans is the most abundant fungus in the oral cavity. Ajwa date seeds, with a concentration of 3.25%, have been shown to strongly inhibit the growth of both bacteria and fungi. This study utilizes effervescent tablets containing Ajwa date seed extract. Purpose: The aim of this study is to evaluate the effect of immersing effervescent tablets containing Ajwa date seed extract on the growth of Streptococcus mutans and Candida albicans on acrylic resin. Methods: The research employed a True Experimental design with a Post Test Only Control Group. A spectrophotometer was used to measure bacterial and fungal growth, and various concentrations of the extract in effervescent tablet preparations were tested. Alkaline peroxide was used as a positive control, while distilled water served as a negative control. Results: The One-Way ANOVA test showed that the smallest bacterial count was observed in the alkaline peroxide group (0.008) and the 13% extract concentration group (0.011). The largest bacterial count was observed in the distilled water group (0.065). The smallest fungal count was also observed in the alkaline peroxide group (0.022), while the largest fungal count was recorded in the distilled water group (0.210). Conclusion: The One-Way ANOVA test revealed significant differences in the number of bacteria and fungi based on the treatment groups.
IDENTIFICATION OF SECONDARY METABOLITES IN AJWA DATE SEED EXTRACT (PHOENIX DACTYLIFERA L.) USING PHYTOCHEMICAL SCREENING, UV-VIS, FTIR, AND GC-MS METHODS Amiruddin, Maqhfirah; Rahmawati, Rahmawati; Chotimah, Chusnul; Vebryansyah, Alhady Putra
Dentino: Jurnal Kedokteran Gigi Vol 11, No 1 (2026)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v11i1.26101

Abstract

ABSTRACT: Previous studies concluded that Ajwa date (Phoenix Dactylifera L.) seed extract significantly reduced the roughness of acrylic resin plates used as denture bases, and has the potential to be used as a raw material for denture cleaning tablets. Several previous studies also showed that Ajwa date seeds contain various secondary metabolites, such as phenolic compounds and flavonoids, which have the potential as antioxidants and other bioactive agents. However, most of these studies were still limited to the use of specific analytical methods separately, such as phytochemical screening or chromatographic analysis, without a comprehensive integrated approach. Objective: To identify the levels of active secondary metabolites in ethanol extract of Ajwa date seeds using a comprehensive method with phytochemical screening, UV-Vis, FTIR, and GC-MS. Results: Ajwa date seeds were found to contain alkaloids, flavonoids, phenolics, tannins, and saponins. UV-Vis analysis showed consistent levels of polyphenols, tannins, and flavonoids. FTIR analysis showed that Ajwa date seeds are a rich natural source of polysaccharides, lipids, and phenolic compounds, and GC-MS results revealed various secondary metabolites detected at various retention times, rich in essential fatty acids, esters, and phenolic derivatives. All test methods were repeated three times. Conclusion: The combination of the four analytical methods complemented each other in identifying and confirming the presence of secondary metabolites in Ajwa date seed extract, thus providing a comprehensive picture of its chemical composition and potential bioactivity. Keywords: Ajwa date seeds, Phytochemical screening, UV-Vis, FTIR, GC-MS