Article Per Year (5 Year)
p-Index From 2021 - 2026
0.61
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Molecular and Cellular Biomedical Sciences (MCBS) Journal of Applied Food Technology
Wardhani, Riuh
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Neuroscience Other

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Exploring Polycarpa aurata Quoy and Gaimard, 1834 Extracts as Antibiotic Candidates: GC-MS Profiling and Molecular Docking Study Sardiani, Nenis; Litaay, Magdalena; Dwyana, Zaraswati; Wardhani, Riuh
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.896-907

Abstract

Marine natural products have garnered global interest due to their remarkable bioactive compounds. The tunicate P. aurata, a highly abundant marine invertebrate, possesses significant bioactive potential with applications as an anti-cancer, antibacterial, and antifungal agent. This study aims to evaluate the potential of P. aurata bioactive compounds as antibiotic candidates through in vitro testing and to explore their activity via an in-silico approach using molecular docking. P. aurata was extracted using maceration, yielding both methanol and n-hexane extracts. The methanol extract of P. aurata demonstrated greater efficacy than the n-hexane extract, particularly against Staphylococcus aureus with an inhibition zone diameter of 18.8 mm, compared to 13 mm for Salmonella typhi, both at a 25% concentration. In comparison, the positive control, ciprofloxacin, produced an inhibition zone ranging from 22 to 24 mm for both bacterial strains. GC-MS analysis of the extract revealed three compounds with high % area and similarity index values: Cyclohexane, 1,3,5-triphenyl, Cholesta-5,22-dien-3-ol, and Cholesta-3,5-diene, all of which were suitable for the selected protein target. Computational analysis through molecular docking demonstrated that these compounds exhibit stronger binding affinities compared to ciprofloxacin. This study suggests that the extract of P. aurata is a promising source of bioactive compounds with substantial therapeutic potential as an antibacterial and antibiotic candidate.
Mechanisms and Applications of Essential Oils as Natural Preservatives in Meat Products: A Review Afidah, Ulil; Wardhani, Riuh; Dewi, Swastika; Purwitasari, Lutfi
Journal of Applied Food Technology Vol 12, No 2 (2025)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jaft.29970

Abstract

The increasing global demand for meat products illustrates the importance of safe and effective preservation methods. Butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and tertiary butylhydroquinone (TBHQ) are common synthetic preservatives that stop microbial growth and lipid oxidation. However, they have been linked to negative health effects. Consequently, natural alternatives such as essential oils (EOs) have gained attention due to their antimicrobial and antioxidant activities. Mechanistically, EOs disrupt microbial cell membranes, inhibit key metabolic enzymes, and scavenge free radicals that initiate lipid oxidation. This review discusses the mechanisms and applications of various essential oils as natural preservatives in meat and meat products. Essential oils, which are high in phenolic compounds, work by breaking down microbial cell membranes, stopping enzymatic systems, and neutralizing free radicals that cause oxidation. Several studies have demonstrated that essential oils (EOs) function as a promising natural preservative in meat products by utilizing specific mechanisms to enhance chemical, microbiological, and sensory quality. For instance, rosemary essential oil (REO), when integrated into vacuum packaging, effectively extends the shelf life of chicken meatballs by inhibiting microbial growth and scavenging free radicals to prevent lipid oxidation. Similarly, oregano essential oil (OEO) exhibits strong antimicrobial action against foodborne pathogens like Salmonella enteritidis and Escherichia coli in dried meat while enhancing sensory properties. In sausage products, nutmeg essential oil (NO) demonstrates its antioxidant capacity by significantly reducing lipid oxidation indicators such as thiobarbituric acid reactive substances (TBARS), suppressed mesophilic bacteria growth, and improved aroma stability during storage. Collectively, these findings confirm that essential oils represent promising natural preservatives that offer a safer multi-target alternative to synthetic additives, especially in the mechanism of cell membrane damage and lipid peroxidation inhibition.
Dual Target Anticancer Potential of Exiguobacterium-derived Metabolites from the Maros-Pangkep Karst: An In Silico Study Targeting EGFR and Caspase-3 Wijaya, Taswin; Haedar, Nur; Widyastuti, Helmy; Wardhani, Riuh; Gani, Fuad
Molecular and Cellular Biomedical Sciences Vol 10, No 1 (2026)
Publisher : Molecular and Cellular Biomedical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v10i1.751

Abstract

Background: Cancer is the second leading cause of death worldwide, and its multifactorial nature limits the effectiveness of single-target therapies. Combination treatments may improve efficacy but are often associated with toxicity and drug interactions. Extremophilic bacteria from karst environments remain underexplored as sources of multitarget anticancer compounds. This study aimed to evaluate the dual-target anticancer potential of bioactive metabolites produced by bacteria isolated from the Maros–Pangkep karst region using molecular docking and ADME analysis.Materials and Methods: A bacterial isolate obtained from soil samples from the Maros–Pangkep karst region, Indonesia, was selected based on colony morphology and identified as Exiguobacterium using 16S rRNA gene analysis. Bioactive compounds were characterized by GC-MS, and three major metabolites were docked against EGFR (PDB ID: 1M17) and caspase-3 (PDB ID: 1PAU). ADME analysis was performed to evaluate drug-likeness and toxicity.Results: Docking analysis showed that compound (1) Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) exhibited the strongest binding affinity toward EGFR (−7.2 kcal/mol), exceeding the native ligand erlotinib (−6.1 kcal/mol). Compound (2) Cyclo(L-prolyl-L-valine) and compound (3) 2,5-piperazinedione,3-methyl-6-(1-methylethyl) derivatives showed binding affinities of −6.4 and −5.9 kcal/mol, respectively. For caspase-3, the compounds displayed binding affinities ranging from −4.5 to −6.0 kcal/mol , exceeding the native ligand Ac-DEVD-CHO (−3.6 kcal/mol) and interacted outside the active site, indicating potential allosteric activation. All compounds complied with Lipinski’s rule of five and showed low toxicity (LD50>800 mg/kg).Conclusion: This study shows that metabolites from Exiguobacterium possess dual-target anticancer potential via EGFR inhibition and caspase-3 modulation, suggesting their use as multitarget drug candidates. Keywords: 16S rRNA, bacteria, biological products, caspase-3, epidermal growth factor receptor, Kart Maros-Pangkep, molecular docking
Co-Authors Afidah, Ulil Gani, Fuad Magdalena Litaay Nur Haedar Purwitasari, Lutfi Sardiani, Nenis Swastika Dewi Widyastuti, Helmy Wijaya, Taswin Zaraswati Dwyana