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All Journal HAYATI Journal of Biosciences Journal of Applied Agricultural Science and Technology Jurnal Pengabdian Masyarakat Bhinneka
Sutama, Daning Kinanti
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Agriculture, Biological Sciences & Forestry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Public Health

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Exhaustive Extraction of Bioactive Components from Sargassum cristaefolium Brown Seaweed: Antioxidant Potential and Bioactivity Rohim, Abd.; Khurniyati, Maylina Ilhami; Sutama, Daning Kinanti; Rahmadina, Safira; Putri, Nabilla Dwi
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.859-878

Abstract

Bioactive components are essential ingredients of functional foods, supplements, pharmaceuticals, etc. Sargassum cristaefolium brown seaweed, as an Indonesian marine resource, provides promising bioactive components. The present study was to extract the total bioactive components of S. cristaefolium with a microwave instrument. The extraction method was done serially using different polarity solvents (1st-stage: non-polar, 2nd-stage: semi-polar, Final-stage: polar). Yielded extracts were analyzed for bioactive compounds, functional groups, total phenolic and flavonoid, and antioxidative activities. The results showed that all staged extractions obtained bioactive compounds with various characteristics. However, the 2nd-stage extract was superior, and it exhibited the highest total phenolic and flavonoid (17.53±0.78 mg GAE/g, 72.64±3.01 mg QE/g), the richest volatile bioactive compounds (neophytadiene and phytol were dominant), and the predominant bioactive compound of antioxidative (oleoylethanolamide). Their functional groups confirmed the structure of antioxidative phenolic molecules: C―C stretching skeleton (phenyl/aromatic core), C―H stretching, C―H bending, and O―H stretching. The strongest primary (1439.84±63.02 µg/ml) and secondary (389.73±16.71 µg/ml) antioxidant activities were presented by the 2nd-stage extract. The efficiency of MAE and the potential of S. cristaefolium were promising for developing functional foods and pharmaceuticals that relate to antioxidants in the future.
Pemberdayaan Masyarakat melalui Pelatihan Kreasi Olahan Mocaf sebagai Inovasi Pangan Lokal Kekinian di Desa Gununggangsir, Beji, Pasuruan Fauziah, Shaddiqah Munawaroh; Sutama, Daning Kinanti; Rohmawati, Uun
Jurnal Pengabdian Masyarakat Bhinneka Vol. 3 No. 4 (2025): Bulan Juli
Publisher : Bhinneka Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58266/jpmb.v3i4.304

Abstract

Tepung mocaf (Modified Cassava Flour) merupakan salah satu produk turunan singkong yang memiliki nilai gizi dan ekonomi tinggi, serta dapat menjadi alternatif bahan pangan lokal yang inovatif. Namun, pemanfaatan mocaf di masyarakat, khususnya di Desa Gununggangsir, Kecamatan Beji, Kabupaten Pasuruan, masih terbatas. Kegiatan pengabdian masyarakat ini bertujuan untuk memberdayakan masyarakat melalui pelatihan kreasi olahan mocaf dengan pendekatan kekinian, guna menarik minat generasi muda dan menciptakan peluang usaha rumahan. Pelatihan difokuskan pada pembuatan berbagai produk olahan seperti brownies mocaf, cookies mocaf, dan donat mocaf, dengan sentuhan tampilan dan rasa yang sesuai dengan tren kuliner masa kini. Metode pelatihan mencakup penyuluhan gizi dan potensi mocaf, demonstrasi resep, serta praktik langsung oleh peserta. Hasil kegiatan menunjukkan bahwa peserta tidak hanya mampu membuat olahan dengan baik, tetapi juga menunjukkan minat untuk mengembangkan usaha kecil dari produk tersebut. Kegiatan ini diharapkan dapat menjadi sarana untuk mengangkat kembali potensi pangan lokal melalui pendekatan inovatif dan meningkatkan kemandirian ekonomi masyarakat desa.
Phytochemical Profile and Antioxidant Activity of Ethanolic Extracts from Selected Wild Medicinal Plants: Azadirachta indica, Acalypha indica, and Muntingia malabura Rizkita, Nadya; Sari, Diana Novita; Sutama, Daning Kinanti
Journal of Applied Agricultural Science and Technology Vol. 9 No. 3 (2025): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v9i3.387

Abstract

Azadirachta indica, Acalypha indica, and Muntingia calabura are wild plants with antioxidant content capable of treating various diseases. This study aims to evaluate phytochemical compound screening using qualitative tests and to characterize them using FT-IR (Fourier Transform Infrared Spectroscopy) in Azadirachta indica, Acalypha indica, and Muntingia Calabura, as well as to assess total phenolic content (TPC), flavonoid content (TFC), and antioxidant potential. The results showed that Azadirachta indica and Acalypha indica contained alkaloids, flavonoids, tannins, saponins, phenols, glycosides, steroids, and carbohydrates but lacked proteins and triterpenoids. In contrast, Muntingia Calabura leaf extract lacked glycosides, triterpenoids, and carbohydrates. The highest TPC content in Muntingia Calabura extract was 269.88±0.00 mg GAE/gr extract, and the highest TFC was found in Azadirachta indica extract at 676.34±21.85 mg CE/gr extract. The ethanol extract of Azadirachta indica and Muntingia Calabura leaves has very strong antioxidant activity, with IC₅₀ values of 32.76 ± 3.02 ppm and 31.16 ± 1.96 ppm, respectively, while Acalypha indica had an IC₅₀ value of 107.71 ± 3.55 ppm, indicating a moderate level of antioxidant activity. These results support the potential of the three medicinal plants as sources of natural antioxidants. The findings can stimulate further research to isolate key bioactive compounds and perform toxicity tests to support potential applications in the pharmaceutical and food industries.
Co-Authors Diana Novita Sari, Diana Novita Maylina Ilhami Khurniyati Putri, Nabilla Dwi Rahmadina, Safira Rizkita, Nadya Rohim, Abd. Rohmawati, Uun Shaddiqah Munawaroh Fauziah