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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Biologi Tropis
Angka Mahardini
Universitas Muhammadiyah Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology

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Mitochondrial COI Haplotype Diversity of Rhynchobatus australiae Collected from Ketapang Fish Port, Bangka Belitung Islands Novita Permata Putri; Irwani Irwani; Munasik Munasik; Ranny Ramadhani Yuneni; Faqih Akbar Alghozali; Angka Mahardini
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 27, No 2 (2022): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.27.2.133-140

Abstract

Rhynchobatus australiae is a member of the Rhinidae family and can be found in the Indo Pacific. This species is categorized as Critically Endangered according to The International Union for Conservation of Nature and Natural Resources (IUCN) Red List and listed as Appendix II Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), due to population declines. Sustainable fisheries management is urgently needed. Information related to genetic diversity is one of the most important aspects of information for appropriate sustainable fisheries management. Therefore, this research aims to investigate the genetic diversity of Rhynchobatus australiae collected from Ketapang Fish Port Bangka Belitung Islands. Total 21 samples were collected at Ketapang Fish Port. DNA extraction was carried out using the 10% chelex method and amplification was done through PCR method on the mitochondrial DNA using Fish BCL and Fish BCH primer. Sequences in size of 653 base pairs were successfully obtained from all fish samples showing the existence of 2 clades from the construction of the phylogeny tree with close genetic distance. Results showed high haplotype diversity (Hd: 0.733 ± 0.082) and low nucleotide diversity (π: 0.00176± 0.00036) with 6 polymorphic sites (S) from 7 unique haplotypes (h). This research provides basic information of genetic diversity of Rhynchobatus australiae collected from Ketapang Fish Port and complements other information to better understand the status of the threatened Rhynchobatus australiae population.
Coral Reef Substrate Coverage in Taka Bonerate National Park Sanova, Aulia Seto Sandhi; Nugroho, Hanityo Adi; Mahardini, Angka; Agus, Elsa Lusia; Rahman, Saleh; Sutanto, Steven; Retawimbi, Aditano Yani
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8575

Abstract

Coral reef monitoring using Reef Check Method has been widely applied for conservation acts due to its handy protocols. This study aims to evaluate the coral reef conditions in the zonal areas of Taka Bonerate National Park by analyzing the substrate composition of its waters. Conducted in November 2023 using the Reef Check method, the research focused on four stations: Tinabo Besar, Tinabo Kecil, Tinanja, and Taka Lasalimu. The substrate composition was categorized into living and non-living substrates at depths of 5 and 10 meters. At 5 meters depth, the highest percentage of living substrate cover, specifically hard coral (HC), was recorded at Tinanja (74%), while the highest non-living substrate cover, dead coral/rubble (RB), was observed at Tinabo Kecil (38%). At 10 meters depth, Tinanja again showed the highest living substrate cover (HC, 40%), whereas Tinabo Kecil had the highest non-living substrate cover (RB, 41%). The findings indicate that the coral reef Substrate in the TBNP zonal area is in good condition, although some locations show damage suspected to be caused by environmentally unfriendly fishing practices. This can serve as a reference for the future management of coral reefs in TNTBR. Further research is needed to comprehensively complete the data on coral reef health, such as data on reef fish and associated invertebrates.
Co-Authors Aditano Yani Retawimbi Agus, Elsa Lusia Faqih Akbar Alghozali Hanityo Adi Nugroho Irwani Irwani Novita Permata Putri Rahman, Saleh Ranny Ramadhani Yuneni Sanova, Aulia Seto Sandhi Sutanto, Steven