Irma Sarita Rahmawati
Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya

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Pengaruh ekstrak tempe kacang tunggak (Vigna Unguiculata (l.) Walp) terhadap kadar malondialdehid pada tikus putih yang diinduksi karbon tetraklorida Lola Ayu Istifiani; Irma Sarita Rahmawati; Rahma Micho Widyanto; Rahmi Nimas Sabrina
Jurnal SAGO Gizi dan Kesehatan Vol 5, No 2 (2024): April
Publisher : Poltekkes Kemenkes Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/gikes.v5i2.1500

Abstract

Background: Free radicals that accumulate in the body can cause degenerative diseases, causing oxidative stress. One of the most damaging impacts of ROS is lipid peroxidation, which produces malondialdehyde (MDA). Consuming foods substantial in antioxidant content, such as Vigna unguiculata (L.) Walp tempeh, which contains antioxidants such as phenolics and flavonoids, can help to reduce MDA levels.Objectives: The aim of this study is to determine the effects of Vigna unguiculata (L.) Walp tempeh extract to reduce MDA levels in rats induced by carbon tetrachloride (CCl4).Methods: This study used a post test only control group design on male white rats (Rattus norvegicus) on male white rats (Rattus norvegicus) induced by CCl4. The research was conducted at the Biosciences Institute Laboratory and the Dietetics and Culinary Laboratory at Brawijaya University from September to October 2022. A sample of 25 male rats was divided into 5 groups, namely negative control, positive control, and the group induced by CCl4 with variations in the administration of cowpea tempeh ethanol extract, namely 3, 6, and 9% for 7 days. After that, the rats blood was taken to analyzed the rats MDA level using a spectrophotometer at a wavelength of 532 nm. Data analysis used One Way ANOVA and continued with post hoc Dunnett t (2-sided) test at 95% CI.Results: There were significant differences in MDA level between groups (p=0,04). MDA levels were significantly (p=0,04) higher in the K+, T1, and T3 groups than in the control group (2,227±0,595). Furthermore, the MDA level in the T2 group (2,838±0,076) was not statistically different from the normal group (p>0,05).Conclusion: This study has shown that administering rats with Vigna unguiculata (L.) Walp tempeh extract has the potential to decrease MDA levels in rats induced by CCl4.Keywords Vigna unguiculata (L.) Walp Tempeh, Oxidative stress, Carbon Tetrachloride, Malondialdehyde
Characterization of texture and microstructure of instant analog rice based on soybean–seaweed formulation Lola Ayu Istifiani; Rahma Micho Widyanto; Aprinia Dian Nurhayati; Irma Sarita Rahmawati; Dita Oktavia Maharani
AcTion: Aceh Nutrition Journal Vol 11, No 2 (2026): June
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v11i2.2938

Abstract

The prevalence of type 2 Diabetes Mellitus (T2DM) continues to rise, and the consumption of high-glycemic index white rice contributes to postprandial hyperglycemia. Alternatives with a low glycemic index that are practical and nutritionally rich remain limited. This study aimed to evaluate the texture and microstructure of instant analog rice based on soybean and seaweed to support T2DM risk management. A completely randomized design with three replicates per treatment was used. The formulations included soybean: seaweed ratios of 40%:60% (P2), 50%:50% (P3), and 60%:40% (P4), along with 100% instant rice as the control (P1). Texture was analyzed using Texture Profile Analysis, and microstructure was observed with a Scanning Electron Microscope (magnification 2000×, 15 kV). Results, significant differences were observed in hardness (P3: 5.21 N), cohesiveness (P2: 0.62), and resilience (P2: 0.41). SEM images showed more porous granules in P2–P4, which correlated with lower hardness and faster rehydration. Formulation P4 (60% soybean:40% seaweed) exhibited moderate hardness (4.87 N), acceptable cohesiveness, and a porous microstructure, supporting its nutritional value and functional properties. In Conclusion, instant analog rice P4 has the potential to serve as a functional food that is practical for T2DM prevention and management, providing a suitable texture and nutritional benefits that support metabolic health.